(Circular) (Six-base) MAPSORT of: pFN21AE6410.seq Check: 5073 from: 1 to: 6109 pFN21AE6410 6109 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2070 2350 2070 Size: 280 5829 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3919 278 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3452 1937 Size: 1515 4594 Acc65I G'GTAC_C Cuts at: 3430 3430 Size: 6109 AclI AA'CG_TT Cuts at: 1563 4236 4609 1563 Size: 2673 373 3063 Fragments arranged by size: 3063 2673 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2987 4172 5556 5824 1632 Size: 1355 1185 1384 268 1917 Fragments arranged by size: 1917 1384 1355 1185 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5912 AflIII A'CryG_T Cuts at: 1203 5008 5985 1203 Size: 3805 977 1327 Fragments arranged by size: 3805 1327 977 AgeI A'CCGG_T Cuts at: 4940 4940 Size: 6109 AhdI GACnn_n'nnGTC Cuts at: 3137 4838 3137 Size: 1701 4408 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6109 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6109 AlwNI CAG_nnn'CTG Cuts at: 3852 5424 3852 Size: 1572 4537 ApaLI G'TGCA_C Cuts at: 4166 5322 4166 Size: 1156 4953 ApoI r'AATT_y Cuts at: 1493 2867 3418 3644 3796 5905 1493 Size: 1374 551 226 152 2109 1697 Fragments arranged by size: 2109 1697 1374 551 226 152 AseI AT'TA_AT Cuts at: 160 1032 4663 160 Size: 872 3631 1606 Fragments arranged by size: 3631 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 AvaI C'yCGr_G Cuts at: 1870 1943 3081 3434 1870 Size: 73 1138 353 4545 Fragments arranged by size: 4545 1138 353 73 AvrII C'CTAG_G Cuts at: 2581 2581 Size: 6109 BamHI G'GATC_C Cuts at: 3439 3806 3439 Size: 367 5742 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2309 2936 3430 4885 Size: 325 217 627 303 219 627 494 1455 Cuts at: 4885 618 Size: 1842 Fragments arranged by size: 1842 1455 627 627 494 325 303 219 217 BanII G_rGCy'C Cuts at: 1391 3428 1391 Size: 2037 4072 BbeI G_GCGC'C Cuts at: 2094 2094 Size: 6109 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4300 4334 1903 Size: 34 2363 34 3678 Fragments arranged by size: 3678 2363 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4001 5211 4001 Size: 1210 4899 BclI T'GATC_A Cuts at: 1548 1548 Size: 6109 BfrBI ATG'CAT Cuts at: 2452 2452 Size: 6109 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2142 4720 Size: 107 122 71 631 605 337 133 2578 Cuts at: 4720 136 Size: 1525 Fragments arranged by size: 2578 1525 631 605 337 133 122 107 71 BglII A'GATC_T Cuts at: 6104 6104 Size: 6109 BlpI GC'TnA_GC Cuts at: 3526 3526 Size: 6109 Bme1580I G_kGCm'C Cuts at: 1790 4170 5326 1790 Size: 2380 1156 2573 Fragments arranged by size: 2573 2380 1156 BmgBI CAC'GTC Cuts at: 1318 2116 2823 3342 1318 Size: 798 707 519 4085 Fragments arranged by size: 4085 798 707 519 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3840 4798 442 Size: 416 53 83 399 24 2423 958 1753 Fragments arranged by size: 2423 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6109 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2252 2542 3148 4769 1239 Size: 1013 290 606 1621 2579 Fragments arranged by size: 2579 1621 1013 606 290 Bpu10I CC'TnA_GC Cuts at: 3335 3335 Size: 6109 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2821 4234 5099 5397 2821 Size: 1413 865 298 3533 Fragments arranged by size: 3533 1413 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2986 4772 882 Size: 617 1487 1786 2219 Fragments arranged by size: 2219 1786 1487 617 BsaAI yAC'GTr Cuts at: 493 1204 3900 493 Size: 711 2696 2702 Fragments arranged by size: 2702 2696 711 BsaBI GATnn'nnATC Cuts at: 6103 6103 Size: 6109 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2091 2370 3916 4298 Size: 53 83 186 936 558 279 1546 382 Cuts at: 4298 4933 275 Size: 635 1451 Fragments arranged by size: 1546 1451 936 635 558 382 279 186 83 53 BsaWI w'CCGG_w Cuts at: 2639 4542 4940 5214 5361 5772 2639 Size: 1903 398 274 147 411 2976 Fragments arranged by size: 2976 1903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2124 2154 2225 2255 2465 Size: 30 165 30 721 30 71 30 210 Cuts at: 2465 2495 1178 Size: 30 4792 Fragments arranged by size: 4792 721 210 165 71 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6109 BseYI C'CCAG_C Cuts at: 5312 5808 5312 Size: 496 5613 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2064 2643 1163 Size: 573 328 579 4629 Fragments arranged by size: 4629 579 573 328 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3559 4320 5348 1540 Size: 275 186 1558 761 1028 2301 Fragments arranged by size: 2301 1558 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4170 4255 5326 4170 Size: 85 1071 4953 Fragments arranged by size: 4953 1071 85 BsmI GAATG_Cn' Cuts at: 2361 3620 3713 2361 Size: 1259 93 4757 Fragments arranged by size: 4757 1259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3428 4170 4255 5326 1391 Size: 399 1638 742 85 1071 2174 Fragments arranged by size: 2174 1638 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2639 5772 2639 Size: 3133 2976 BspHI T'CATG_A Cuts at: 2185 3998 5728 2185 Size: 1813 1730 2566 Fragments arranged by size: 2566 1813 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2070 2204 2350 3464 844 Size: 1226 134 146 1114 3489 Fragments arranged by size: 3489 1226 1114 146 134 BsrBI CCG'CTC Cuts at: 1394 1823 2788 3996 1394 Size: 429 965 1208 3507 Fragments arranged by size: 3507 1208 965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4604 4778 65 Size: 1183 3356 174 1396 Fragments arranged by size: 3356 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4753 4940 1700 Size: 110 2943 187 2869 Fragments arranged by size: 2943 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6109 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6109 BssSI C'ACGA_G Cuts at: 4169 5181 4169 Size: 1012 5097 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6109 BstBI TT'CG_AA Cuts at: 4913 4913 Size: 6109 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6109 BstYI r'GATC_y Cuts at: 1972 3439 3806 4191 4208 5660 5746 5758 6104 Size: 1467 367 385 17 1452 86 12 346 Cuts at: 6104 1972 Size: 1977 Fragments arranged by size: 1977 1467 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 513 Size: 552 939 147 4471 Fragments arranged by size: 4471 939 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5370 Fragments arranged by size: 5370 683 56 BtsI GCAGTG_nn' Cuts at: 3264 3372 3625 4419 4439 3264 Size: 108 253 794 20 4934 Fragments arranged by size: 4934 794 253 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6109 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6074 DraI TTT'AAA Cuts at: 3413 3413 Size: 6109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5116 809 Size: 507 3800 1802 Fragments arranged by size: 3800 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2550 3556 4445 Size: 54 1425 50 275 18 720 1006 889 Cuts at: 4445 6074 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1006 889 720 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3556 1537 Size: 275 1744 4090 Fragments arranged by size: 4090 1744 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3276 4039 1353 Size: 1923 763 3423 Fragments arranged by size: 3423 1923 763 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4696 5070 5216 387 Size: 1064 138 3107 374 146 1280 Fragments arranged by size: 3107 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 2542 2987 3148 4172 4769 5556 Size: 393 620 290 445 161 1024 597 787 Cuts at: 5556 5824 1239 Size: 268 1524 Fragments arranged by size: 1524 1024 787 620 597 445 393 290 268 161 EcoNI CCTnn'n_nnAGG Cuts at: 2077 2077 Size: 6109 EcoO109I rG'GnC_Cy Cuts at: 2383 2383 Size: 6109 EcoRI G'AATT_C Cuts at: 3418 3796 3418 Size: 378 5731 EcoRV GAT'ATC Cuts at: 3160 3160 Size: 6109 FspI TGC'GCA Cuts at: 4615 4615 Size: 6109 HaeII r_GCGC'y Cuts at: 1678 2094 2148 3092 5256 1678 Size: 416 54 944 2164 2531 Fragments arranged by size: 2531 2164 944 416 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2360 2392 2465 Size: 32 135 32 653 32 103 32 73 Cuts at: 2465 2497 3123 3155 4750 4782 4824 4856 1373 Size: 32 626 32 1595 32 42 32 2626 Fragments arranged by size: 2626 1595 653 626 135 103 73 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2884 3142 3453 3699 669 Size: 988 281 946 258 311 246 3079 Fragments arranged by size: 3079 988 946 311 281 258 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6109 HpaI GTT'AAC Cuts at: 2884 3699 2884 Size: 815 5294 KasI G'GCGC_C Cuts at: 2090 2090 Size: 6109 KpnI G_GTAC'C Cuts at: 3434 3434 Size: 6109 MfeI C'AATT_G Cuts at: 3708 3708 Size: 6109 MluI A'CGCG_T Cuts at: 5985 5985 Size: 6109 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2451 2638 5223 5407 1305 Size: 1146 187 2585 184 2007 Fragments arranged by size: 2585 2007 1146 187 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4287 Fragments arranged by size: 4287 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2184 2832 4067 4426 4585 518 Size: 648 1018 648 1235 359 159 2042 Fragments arranged by size: 2042 1235 1018 648 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2065 2933 2995 3525 4202 5350 5595 Size: 452 7 868 62 530 677 1148 245 Cuts at: 5595 1606 Size: 2120 Fragments arranged by size: 2120 1148 868 677 530 452 245 62 7 NarI GG'CG_CC Cuts at: 2091 2091 Size: 6109 NcoI C'CATG_G Cuts at: 513 1065 2004 2151 513 Size: 552 939 147 4471 Fragments arranged by size: 4471 939 552 147 NdeI CA'TA_TG Cuts at: 387 387 Size: 6109 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6109 NotI GC'GGCC_GC Cuts at: 3556 3556 Size: 6109 NsiI A_TGCA'T Cuts at: 2454 2454 Size: 6109 NspI r_CATG'y Cuts at: 1132 1229 2456 2837 3062 3467 5012 1132 Size: 97 1227 381 225 405 1545 2229 Fragments arranged by size: 2229 1545 1227 405 381 225 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6109 PciI A'CATG_T Cuts at: 5008 5008 Size: 6109 PflMI CCAn_nnn'nTGG Cuts at: 1108 2565 1108 Size: 1457 4652 PmeI GTTT'AAAC Cuts at: 3413 3413 Size: 6109 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2481 2513 4178 4210 5691 5723 2481 Size: 32 1665 32 1481 32 2867 Fragments arranged by size: 2867 1665 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6109 PsiI TTA'TAA Cuts at: 3679 3679 Size: 6109 PstI C_TGCA'G Cuts at: 830 2949 3461 830 Size: 2119 512 3478 Fragments arranged by size: 3478 2119 512 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6109 PvuII CAG'CTG Cuts at: 2933 2995 2933 Size: 62 6047 SalI G'TCGA_C Cuts at: 1936 3451 1936 Size: 1515 4594 SapI GCTCTTCn'nnn_ Cuts at: 3276 3276 Size: 6109 SbfI CC_TGCA'GG Cuts at: 3461 3461 Size: 6109 ScaI AGT'ACT Cuts at: 2229 4357 6069 2229 Size: 2128 1712 2269 Fragments arranged by size: 2269 2128 1712 SfcI C'TryA_G Cuts at: 826 1045 2503 2945 3282 3457 4592 5273 5464 Size: 219 1458 442 337 175 1135 681 191 Cuts at: 5464 826 Size: 1471 Fragments arranged by size: 1471 1458 1135 681 442 337 219 191 175 SfoI GGC'GCC Cuts at: 2092 2092 Size: 6109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6109 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6109 SmaI CCC'GGG Cuts at: 1872 3436 1872 Size: 1564 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2800 4213 5114 5376 820 Size: 197 926 857 1413 901 262 1553 Fragments arranged by size: 1553 1413 926 901 857 262 197 SnaBI TAC'GTA Cuts at: 493 3900 493 Size: 3407 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6109 SphI G_CATG'C Cuts at: 1132 2456 3467 1132 Size: 1324 1011 3774 Fragments arranged by size: 3774 1324 1011 SspI AAT'ATT Cuts at: 5 52 4033 5 Size: 47 3981 2081 Fragments arranged by size: 3981 2081 47 StuI AGG'CCT Cuts at: 1517 2136 3079 1517 Size: 619 943 4547 Fragments arranged by size: 4547 943 619 StyI C'CwwG_G Cuts at: 513 1065 2004 2151 2163 2564 2581 2808 3548 Size: 552 939 147 12 401 17 227 740 Cuts at: 3548 513 Size: 3074 Fragments arranged by size: 3074 939 740 552 401 227 147 17 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2502 4136 4153 4306 4491 4936 325 Size: 1255 922 1634 17 153 185 445 1498 Fragments arranged by size: 1634 1498 1255 922 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2227 4355 6067 Size: 274 80 33 51 1443 250 2128 1712 Cuts at: 6067 96 Size: 138 Fragments arranged by size: 2128 1712 1443 274 250 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2124 2156 6069 6101 2124 Size: 32 3913 32 2132 Fragments arranged by size: 3913 2132 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3255 1316 Size: 1939 4170 XbaI T'CTAG_A Cuts at: 3445 3445 Size: 6109 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6109 XmaI C'CCGG_G Cuts at: 1870 3434 1870 Size: 1564 4545 XmnI GAAnn'nnTTC Cuts at: 4238 4238 Size: 6109 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3917 276 Size: 53 83 186 936 2383 2468 Fragments arranged by size: 2468 2383 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI