(Circular) (Six-base) MAPSORT of: pFN21AE1053.seq Check: 8046 from: 1 to: 5833 pFN21AE1053 5833 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2784 3036 2784 Size: 252 5581 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2621 2731 2815 3896 5280 5548 1632 Size: 989 110 84 1081 1384 268 1917 Fragments arranged by size: 1917 1384 1081 989 268 110 84 AfeI AGC'GCT Cuts at: 2660 2660 Size: 5833 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 2874 1166 Size: 1708 4125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 2462 2776 2866 3576 5148 2462 Size: 314 90 710 1572 3147 Fragments arranged by size: 3147 1572 710 314 90 ApaI G_GGCC'C Cuts at: 2338 2469 2338 Size: 131 5702 ApaLI G'TGCA_C Cuts at: 3119 3890 5046 3119 Size: 771 1156 3906 Fragments arranged by size: 3906 1156 771 ApoI r'AATT_y Cuts at: 1493 3142 3368 3520 5629 1493 Size: 1649 226 152 2109 1697 Fragments arranged by size: 2109 1697 1649 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2468 2480 3158 1870 Size: 73 525 12 678 4545 Fragments arranged by size: 4545 678 525 73 12 AvrII C'CTAG_G Cuts at: 2014 2014 Size: 5833 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2426 3024 3065 3154 4609 Size: 325 217 627 639 598 41 89 1455 Cuts at: 4609 618 Size: 1842 Fragments arranged by size: 1842 1455 639 627 598 325 217 89 41 BanII G_rGCy'C Cuts at: 1391 2238 2338 2469 2843 2927 3095 3152 1391 Size: 847 100 131 374 84 168 57 4072 Fragments arranged by size: 4072 847 374 168 131 100 84 57 BbeI G_GCGC'C Cuts at: 2430 3069 2430 Size: 639 5194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BbvCI CC'TCA_GC Cuts at: 2100 2100 Size: 5833 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2471 2505 3064 3098 4024 4058 1903 Size: 34 534 34 559 34 926 34 3678 Fragments arranged by size: 3678 926 559 534 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 1548 Size: 5833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 2736 2904 Size: 107 122 71 631 605 854 210 168 Cuts at: 2904 4444 136 Size: 1540 1525 Fragments arranged by size: 1540 1525 854 631 605 210 168 122 107 71 BglII A'GATC_T Cuts at: 5828 5828 Size: 5833 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 2338 2469 3123 3894 5050 1790 Size: 548 131 654 771 1156 2573 Fragments arranged by size: 2573 1156 771 654 548 131 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2941 4493 1239 Size: 886 816 1552 2579 Fragments arranged by size: 2579 1552 886 816 Bpu10I CC'TnA_GC Cuts at: 2100 2100 Size: 5833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2427 3066 3640 4022 Size: 53 83 186 936 894 639 574 382 Cuts at: 4022 4657 275 Size: 635 1451 Fragments arranged by size: 1451 936 894 639 635 574 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2963 2993 1178 Size: 30 165 30 1560 30 4018 Fragments arranged by size: 4018 1560 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2332 1186 Size: 1146 4687 BseYI C'CCAG_C Cuts at: 2085 2160 2415 2462 2497 3096 5036 5532 2085 Size: 75 255 47 35 599 1940 496 2386 Fragments arranged by size: 2386 1940 599 496 255 75 47 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2362 2468 2852 1163 Size: 573 626 106 384 4144 Fragments arranged by size: 4144 626 573 384 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2381 2515 2740 3007 3283 4044 Size: 275 186 380 134 225 267 276 761 Cuts at: 4044 5072 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 380 276 275 267 225 186 134 BsiHKAI G_wGCw'C Cuts at: 2157 2843 2927 2934 2943 2981 3095 3123 3894 Size: 686 84 7 9 38 114 28 771 Cuts at: 3894 3979 5050 2157 Size: 85 1071 2940 Fragments arranged by size: 2940 1071 771 686 114 85 84 38 28 9 7 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2238 2338 2469 2843 2927 2934 Size: 399 367 81 100 131 374 84 7 Cuts at: 2934 2943 2981 3095 3123 3152 3894 3979 5050 Size: 9 38 114 28 29 742 85 1071 Cuts at: 5050 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 374 367 131 114 100 85 84 81 38 29 28 9 7 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2784 3036 3188 844 Size: 1940 252 152 3489 Fragments arranged by size: 3489 1940 252 152 BsrBI CCG'CTC Cuts at: 1394 1823 2377 2485 3009 3720 1394 Size: 429 554 108 524 711 3507 Fragments arranged by size: 3507 711 554 524 429 108 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2348 2380 2520 4477 4664 1700 Size: 110 538 32 140 1957 187 2869 Fragments arranged by size: 2869 1957 538 187 140 110 32 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 2532 1925 Size: 607 5226 BssSI C'ACGA_G Cuts at: 2093 2444 2645 3893 4905 2093 Size: 351 201 1248 1012 3021 Fragments arranged by size: 3021 1248 1012 351 201 BstAPI GCAn_nnn'nTGC Cuts at: 794 2776 2901 794 Size: 1982 125 3726 Fragments arranged by size: 3726 1982 125 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstXI CCAn_nnnn'nTGG Cuts at: 2396 2396 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2176 3163 3530 3915 3932 5384 5470 5482 Size: 204 987 367 385 17 1452 86 12 Cuts at: 5482 5828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 987 385 367 346 204 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2148 2315 2148 Size: 167 5666 BtgI C'CryG_G Cuts at: 513 1065 2004 2113 2420 2665 3001 513 Size: 552 939 109 307 245 336 3345 Fragments arranged by size: 3345 939 552 336 307 245 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 3349 4143 4163 3349 Size: 794 20 5019 Fragments arranged by size: 5019 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2378 2728 2737 Size: 54 1425 50 275 18 548 350 9 Cuts at: 2737 2821 3004 3085 3280 4169 5798 8 Size: 84 183 81 195 889 1629 43 Fragments arranged by size: 1629 1425 889 548 350 275 195 183 84 81 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2378 2737 3004 3280 1537 Size: 275 566 359 267 276 4090 Fragments arranged by size: 4090 566 359 276 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 3763 1353 Size: 2410 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2841 2925 3093 2841 Size: 84 168 5581 Fragments arranged by size: 5581 168 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2621 2731 2815 2941 3896 4493 Size: 393 493 496 110 84 126 955 597 Cuts at: 4493 5280 5548 1239 Size: 787 268 1524 Fragments arranged by size: 1524 955 787 597 496 493 393 268 126 110 84 EcoO109I rG'GnC_Cy Cuts at: 2275 2334 2275 Size: 59 5774 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 2306 2780 4339 2306 Size: 474 1559 3800 Fragments arranged by size: 3800 1559 474 HaeII r_GCGC'y Cuts at: 1678 2303 2349 2430 2662 3069 4980 1678 Size: 625 46 81 232 407 1911 2531 Fragments arranged by size: 2531 1911 625 407 232 81 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4474 4506 4548 4580 1373 Size: 32 135 32 2902 32 42 32 2626 Fragments arranged by size: 2902 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3177 3423 669 Size: 988 281 568 671 246 3079 Fragments arranged by size: 3079 988 671 568 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KasI G'GCGC_C Cuts at: 2426 3065 2426 Size: 639 5194 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 2495 2868 3006 4947 5131 1305 Size: 888 302 373 138 1941 184 2007 Fragments arranged by size: 2007 1941 888 373 302 184 138 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 2124 2874 3791 4150 4309 518 Size: 648 946 12 750 917 359 159 2042 Fragments arranged by size: 2042 946 917 750 648 359 159 12 MspA1I CmG'CkG Cuts at: 1606 2422 2459 2667 2863 3003 3115 3249 3926 Size: 816 37 208 196 140 112 134 677 Cuts at: 3926 5074 5319 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 245 208 196 140 134 112 37 NaeI GCC'GGC Cuts at: 2350 2382 2522 2350 Size: 32 140 5661 Fragments arranged by size: 5661 140 32 NarI GG'CG_CC Cuts at: 2427 3066 2427 Size: 639 5194 NcoI C'CATG_G Cuts at: 513 1065 2004 2113 513 Size: 552 939 109 4233 Fragments arranged by size: 4233 939 552 109 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NgoMIV G'CCGG_C Cuts at: 2348 2380 2520 2348 Size: 32 140 5661 Fragments arranged by size: 5661 140 32 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 2737 3004 3280 2737 Size: 267 276 5290 Fragments arranged by size: 5290 276 267 NspI r_CATG'y Cuts at: 1132 1229 3191 4736 1132 Size: 97 1962 1545 2229 Fragments arranged by size: 2229 1962 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PspOMI G'GGCC_C Cuts at: 2334 2465 2334 Size: 131 5702 PstI C_TGCA'G Cuts at: 830 2897 3185 830 Size: 2067 288 3478 Fragments arranged by size: 3478 2067 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SacI G_AGCT'C Cuts at: 2843 2927 3095 2843 Size: 84 168 5581 Fragments arranged by size: 5581 168 84 SacII CC_GC'GG Cuts at: 2423 2668 3004 2423 Size: 245 336 5252 Fragments arranged by size: 5252 336 245 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SexAI A'CCwGG_T Cuts at: 2847 2847 Size: 5833 SfcI C'TryA_G Cuts at: 826 1045 2893 3181 4316 4997 5188 826 Size: 219 1848 288 1135 681 191 1471 Fragments arranged by size: 1848 1471 1135 681 288 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2736 2736 Size: 5833 SfoI GGC'GCC Cuts at: 2428 3067 2428 Size: 639 5194 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 2564 2828 2912 3080 1517 Size: 1047 264 84 168 4270 Fragments arranged by size: 4270 1047 264 168 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2014 2113 3272 513 Size: 552 939 10 99 1159 3074 Fragments arranged by size: 3074 1159 939 552 99 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 3860 3877 4030 4215 4660 325 Size: 1255 921 1359 17 153 185 445 1498 Fragments arranged by size: 1498 1359 1255 921 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5793 5825 5793 Size: 32 5801 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3053 3962 3053 Size: 909 4924 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SanDI SapI SrfI SwaI XcmI