################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AccIII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvrII BbeI BbrPI BbsI BclI BlnI BlpI Bpu1102I BpuAI BsaI BsaWI BseAI BsiWI BsmBI BsmI BspEI BspLU11I BsrBI BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CpoI DraI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KasI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 930 GACGTC GACGT^C Acc65I 188 GGTACC G^GTACC AccI 409 GTCGAC GT^[AC][GT]AC AclI 1258 AACGTT AA^CGTT AcsI 1761 GAATTT [GA]^AATT[CT] AflII 838 CTTAAG C^TTAAG ApaI 478 GGGCCC GGGCC^C ApoI 1761 GAATTT [GA]^AATT[CT] Asp718I 188 GGTACC G^GTACC AspHI 1750 GAGCTC G[AT]GC[AT]^C AviII 344 TGCGCA TGC^GCA BbvCI 360 GCTGAGG CCTCAGC(-5/-2) BfrI 838 CTTAAG C^TTAAG BglII 740 AGATCT A^GATCT BsaAI 1738 TACGTG [CT]AC^GT[GA] BsaBI 1392 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 178 GAGGAG GAGGAG(10/8) BsiHKAI 1750 GAGCTC G[AT]GC[AT]^C BsmAI 1836 GAGAC GTCTC(1/5) Bsp1407I 659 TGTACA T^GTACA BspHI 1775 TCATGA T^CATGA BsrFI 191 ACCGGC [GA]^CCGG[CT] BsrGI 659 TGTACA T^GTACA BssHII 56 GCGCGC G^CGCGC Cfr10I 191 ACCGGC [GA]^CCGG[CT] EagI 196 CGGCCG C^GGCCG EarI 1675 GAAGAG CTCTTC(1/4) EclXI 196 CGGCCG C^GGCCG Eco52I 196 CGGCCG C^GGCCG EcoNI 465 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 344 TGCGCA TGC^GCA KpnI 188 GGTACC GGTAC^C Ksp632I 1675 GAAGAG CTCTTC(1/4) KspI 1322 CCGCGG CCGC^GG MamI 1392 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 839 TTAA T^TAA NotI 195 GCGGCCGC GC^GGCCGC PflMI 389 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 208 GAGTC GAGTC(4/5) PshAI 265 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1258 AACGTT AA^CGTT RcaI 1775 TCATGA T^CATGA SacI 1750 GAGCTC GAGCT^C SacII 1322 CCGCGG CCGC^GG SalI 409 GTCGAC G^TCGAC SmaI 5 CCCGGG CCC^GGG SmlI 838 CTTAAG C^T[CT][GA]AG SspBI 659 TGTACA T^GTACA Tru9I 839 TTAA T^TAA Van91I 389 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 5 CCCGGG C^CCGGG XmaI 5 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AlwNI 337 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 855 CAGCCTCTG AvaI 5 CCCGGG C^[CT]CG[GA]G 34 CTCGGG BalI 1343 TGGCCA TGG^CCA 1562 TGGCCA BamHI 974 GGATCC G^GATCC 1653 GGATCC BcgI 882 CGAACGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1793 CGACGAGAGTGC BciVI 582 GTATCC GTATCC(6/5) 1902 GTATCC BglI 22 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 486 GCCCTCCCGGC BsiEI 196 CGGCCG CG[GA][CT]^CG 1325 CGGTCG BsoBI 5 CCCGGG C^[CT]CG[GA]G 34 CTCGGG BspDI 682 ATCGAT AT^CGAT 1210 ATCGAT BstXI 701 CCACCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1409 CCAAATCCTTGG ClaI 682 ATCGAT AT^CGAT 1210 ATCGAT DdeI 361 CTGAG C^T[ACGT]AG 1911 CTCAG DrdI 688 GACATCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1779 GACTGAACAGTC EcoT14I 587 CCAAGG C^C[AT][AT]GG 1415 CCTTGG HaeII 30 GGCGCT [GA]GCGC^[CT] 1236 GGCGCT HincII 409 GTCGAC GT[CT]^[GA]AC 1042 GTCAAC HindII 409 GTCGAC GT[CT]^[GA]AC 1042 GTCAAC MluNI 1343 TGGCCA TGG^CCA 1562 TGGCCA MscI 1343 TGGCCA TGG^CCA 1562 TGGCCA NspI 227 ACATGC [GA]CATG^[CT] 1277 ACATGC PpuMI 338 AGGACCT [GA]G^G[AT]CC[CT] 823 GGGACCC SexAI 464 ACCTGGT A^CC[AT]GGT 1897 ACCAGGT StyI 587 CCAAGG C^C[AT][AT]GG 1415 CCTTGG Tsp509I 733 AATT ^AATT 1762 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI Asp700I AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII XmnI