(Circular) (Six-base) MAPSORT of: pFN21AE4549.seq Check: 7424 from: 1 to: 6658 pFN21AE4549 6658 bp With 182 enzymes: SgfI * March 28, 2012 14:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3277 3277 Size: 6658 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2939 4468 278 Size: 53 83 186 936 1403 1529 2468 Fragments arranged by size: 2468 1529 1403 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2415 4001 1937 Size: 478 1586 4594 Fragments arranged by size: 4594 1586 478 Acc65I G'GTAC_C Cuts at: 2193 3979 2193 Size: 1786 4872 AclI AA'CG_TT Cuts at: 1563 3264 4785 5158 1563 Size: 1701 1521 373 3063 Fragments arranged by size: 3063 1701 1521 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3699 4721 6105 6373 1632 Size: 2067 1022 1384 268 1917 Fragments arranged by size: 2067 1917 1384 1022 268 AflII C'TTAA_G Cuts at: 820 1017 2843 820 Size: 197 1826 4635 Fragments arranged by size: 4635 1826 197 AflIII A'CryG_T Cuts at: 1203 5557 6534 1203 Size: 4354 977 1327 Fragments arranged by size: 4354 1327 977 AgeI A'CCGG_T Cuts at: 5489 5489 Size: 6658 AhdI GACnn_n'nnGTC Cuts at: 5387 5387 Size: 6658 AleI CACnn'nnGTG Cuts at: 1166 2357 2711 1166 Size: 1191 354 5113 Fragments arranged by size: 5113 1191 354 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2943 3078 1184 Size: 1759 135 4764 Fragments arranged by size: 4764 1759 135 AlwNI CAG_nnn'CTG Cuts at: 2347 2865 4401 5973 2347 Size: 518 1536 1572 3032 Fragments arranged by size: 3032 1572 1536 518 ApaI G_GGCC'C Cuts at: 2487 2487 Size: 6658 ApaLI G'TGCA_C Cuts at: 4715 5871 4715 Size: 1156 5502 ApoI r'AATT_y Cuts at: 1493 3766 3967 4193 4345 6454 1493 Size: 2273 201 226 152 2109 1697 Fragments arranged by size: 2273 2109 1697 226 201 152 AseI AT'TA_AT Cuts at: 160 1032 5212 160 Size: 872 4180 1606 Fragments arranged by size: 4180 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2039 3983 1870 Size: 73 67 29 1944 4545 Fragments arranged by size: 4545 1944 73 67 29 BamHI G'GATC_C Cuts at: 2979 3658 3988 4355 2979 Size: 679 330 367 5282 Fragments arranged by size: 5282 679 367 330 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2193 2848 3134 3540 3979 Size: 325 217 627 406 655 286 406 439 Cuts at: 3979 5434 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 655 627 439 406 406 325 286 217 BanII G_rGCy'C Cuts at: 1391 2105 2487 3484 3759 3977 1391 Size: 714 382 997 275 218 4072 Fragments arranged by size: 4072 997 714 382 275 218 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5687 BbvCI CC'TCA_GC Cuts at: 2366 2366 Size: 6658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2876 2910 3787 3821 4849 4883 1903 Size: 34 939 34 877 34 1028 34 3678 Fragments arranged by size: 3678 1028 939 877 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2598 3918 4550 5760 2598 Size: 1320 632 1210 3496 Fragments arranged by size: 3496 1320 1210 632 BclI T'GATC_A Cuts at: 1548 1548 Size: 6658 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2033 2497 5269 Size: 107 122 71 631 605 361 464 2772 Cuts at: 5269 136 Size: 1525 Fragments arranged by size: 2772 1525 631 605 464 361 122 107 71 BglII A'GATC_T Cuts at: 2745 6653 2745 Size: 3908 2750 BlpI GC'TnA_GC Cuts at: 4075 4075 Size: 6658 Bme1580I G_kGCm'C Cuts at: 1790 2383 2487 3543 4719 5875 1790 Size: 593 104 1056 1176 1156 2573 Fragments arranged by size: 2573 1176 1156 1056 593 104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6658 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2835 2951 3568 Size: 416 53 83 399 24 1418 116 617 Cuts at: 3568 4389 5347 442 Size: 821 958 1753 Fragments arranged by size: 1753 1418 958 821 617 416 399 116 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6658 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2158 2230 3566 5318 1239 Size: 919 72 1336 1752 2579 Fragments arranged by size: 2579 1752 1336 919 72 Bpu10I CC'TnA_GC Cuts at: 2366 2366 Size: 6658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4783 5648 5946 4783 Size: 865 298 5495 Fragments arranged by size: 5495 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5321 882 Size: 617 3822 2219 Fragments arranged by size: 3822 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3745 4449 493 Size: 711 2541 704 2702 Fragments arranged by size: 2702 2541 711 704 BsaBI GATnn'nnATC Cuts at: 3401 6652 3401 Size: 3251 3407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2170 2275 2936 4465 Size: 53 83 186 936 637 105 661 1529 Cuts at: 4465 4847 5482 275 Size: 382 635 1451 Fragments arranged by size: 1529 1451 936 661 637 635 382 186 105 83 53 BsaWI w'CCGG_w Cuts at: 5091 5489 5763 5910 6321 5091 Size: 398 274 147 411 5428 Fragments arranged by size: 5428 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2177 2207 1178 Size: 30 165 30 687 30 57 30 5629 Fragments arranged by size: 5629 687 165 57 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 1186 Size: 1012 5646 BseYI C'CCAG_C Cuts at: 2065 2295 3808 5861 6357 2065 Size: 230 1513 2053 496 2366 Fragments arranged by size: 2366 2053 1513 496 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2913 2939 3876 1163 Size: 573 1177 26 937 3945 Fragments arranged by size: 3945 1177 937 573 26 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2204 3333 4108 4869 5897 1540 Size: 275 186 203 1129 775 761 1028 2301 Fragments arranged by size: 2301 1129 1028 775 761 275 203 186 BsiHKAI G_wGCw'C Cuts at: 3759 4719 4804 5875 3759 Size: 960 85 1071 4542 Fragments arranged by size: 4542 1071 960 85 BsmI GAATG_Cn' Cuts at: 4169 4262 4169 Size: 93 6565 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 2383 2487 3484 3543 3759 3977 Size: 399 315 278 104 997 59 216 218 Cuts at: 3977 4719 4804 5875 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 997 742 399 315 278 218 216 104 85 59 BspEI T'CCGG_A Cuts at: 6321 6321 Size: 6658 BspHI T'CATG_A Cuts at: 3780 4547 6277 3780 Size: 767 1730 4161 Fragments arranged by size: 4161 1730 767 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2355 3126 3277 4013 844 Size: 1511 771 151 736 3489 Fragments arranged by size: 3489 1511 771 736 151 BsrBI CCG'CTC Cuts at: 1394 1823 4545 1394 Size: 429 2722 3507 Fragments arranged by size: 3507 2722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5153 5327 65 Size: 1183 3905 174 1396 Fragments arranged by size: 3905 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2196 5302 5489 1700 Size: 110 386 3106 187 2869 Fragments arranged by size: 3106 2869 386 187 110 BsrGI T'GTAC_A Cuts at: 96 2664 96 Size: 2568 4090 BssHII G'CGCG_C Cuts at: 1925 2061 1925 Size: 136 6522 BssSI C'ACGA_G Cuts at: 4718 5730 4718 Size: 1012 5646 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6658 BstBI TT'CG_AA Cuts at: 5462 5462 Size: 6658 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6658 BstXI CCAn_nnnn'nTGG Cuts at: 2713 3421 2713 Size: 708 5950 BstYI r'GATC_y Cuts at: 1972 2745 2979 3658 3825 3988 4355 4740 4757 Size: 773 234 679 167 163 367 385 17 Cuts at: 4757 6209 6295 6307 6653 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 773 679 385 367 346 234 167 163 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2226 3040 3327 513 Size: 552 939 222 814 287 3844 Fragments arranged by size: 3844 939 814 552 287 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2347 487 Size: 683 56 1121 4798 Fragments arranged by size: 4798 1121 683 56 BtsI GCAGTG_nn' Cuts at: 2195 2383 4174 4968 4988 2195 Size: 188 1791 794 20 3865 Fragments arranged by size: 3865 1791 794 188 20 ClaI AT'CG_AT Cuts at: 1554 2688 3216 1554 Size: 1134 528 4996 Fragments arranged by size: 4996 1134 528 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3559 3594 529 Size: 35 2995 35 3593 Fragments arranged by size: 3593 2995 35 35 DraI TTT'AAA Cuts at: 3962 3962 Size: 6658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2699 3790 5665 809 Size: 507 1383 1091 1875 1802 Fragments arranged by size: 1875 1802 1383 1091 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2201 2870 3092 Size: 54 1425 50 275 18 371 669 222 Cuts at: 3092 3348 3567 4105 4994 6623 8 Size: 256 219 538 889 1629 43 Fragments arranged by size: 1629 1425 889 669 538 371 275 256 222 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2201 4105 1537 Size: 275 389 1904 4090 Fragments arranged by size: 4090 1904 389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3675 4588 1353 Size: 2322 913 3423 Fragments arranged by size: 3423 2322 913 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5245 5619 5765 387 Size: 1064 138 3656 374 146 1280 Fragments arranged by size: 3656 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3757 3757 Size: 6658 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2158 2230 3566 3699 4721 5318 6105 Size: 393 526 72 1336 133 1022 597 787 Cuts at: 6105 6373 1239 Size: 268 1524 Fragments arranged by size: 1524 1336 1022 787 597 526 393 268 133 72 EcoNI CCTnn'n_nnAGG Cuts at: 2474 2474 Size: 6658 EcoO109I rG'GnC_Cy Cuts at: 2219 2344 2483 2484 2829 2945 2219 Size: 125 139 1 345 116 5932 Fragments arranged by size: 5932 345 139 125 116 1 EcoRI G'AATT_C Cuts at: 3967 4345 3967 Size: 378 6280 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2617 2804 3844 2617 Size: 187 1040 5431 Fragments arranged by size: 5431 1040 187 FspI TGC'GCA Cuts at: 2351 5164 2351 Size: 2813 3845 HaeII r_GCGC'y Cuts at: 1678 2039 3245 5805 1678 Size: 361 1206 2560 2531 Fragments arranged by size: 2560 2531 1206 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 3347 3379 3817 Size: 32 135 32 518 32 1225 32 438 Cuts at: 3817 3849 5299 5331 5373 5405 1373 Size: 32 1450 32 42 32 2626 Fragments arranged by size: 2626 1450 1225 518 438 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2416 3049 4002 4248 669 Size: 988 281 478 633 953 246 3079 Fragments arranged by size: 3079 988 953 633 478 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6658 HpaI GTT'AAC Cuts at: 4248 4248 Size: 6658 KpnI G_GTAC'C Cuts at: 2197 3983 2197 Size: 1786 4872 MfeI C'AATT_G Cuts at: 4257 4257 Size: 6658 MluI A'CGCG_T Cuts at: 6534 6534 Size: 6658 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2757 3730 5772 5956 1305 Size: 1452 973 2042 184 2007 Fragments arranged by size: 2042 2007 1452 973 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3350 3569 10 Size: 54 1425 343 1518 219 3099 Fragments arranged by size: 3099 1518 1425 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2388 2711 3060 3287 4616 4975 Size: 648 1191 31 323 349 227 1329 359 Cuts at: 4975 5134 518 Size: 159 2042 Fragments arranged by size: 2042 1329 1191 648 359 349 323 227 159 31 MspA1I CmG'CkG Cuts at: 1606 2206 2650 2923 3097 3329 3559 3565 4074 Size: 600 444 273 174 232 230 6 509 Cuts at: 4074 4751 5899 6144 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 600 509 444 273 245 232 230 174 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5167 Fragments arranged by size: 5167 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6658 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6658 NotI GC'GGCC_GC Cuts at: 2201 4105 2201 Size: 1904 4754 NspI r_CATG'y Cuts at: 1132 1229 2236 3286 4016 5561 1132 Size: 97 1007 1050 730 1545 2229 Fragments arranged by size: 2229 1545 1050 1007 730 97 PasI CC'CwG_GG Cuts at: 1348 2833 1348 Size: 1485 5173 PciI A'CATG_T Cuts at: 5557 5557 Size: 6658 PflMI CCAn_nnn'nTGG Cuts at: 1108 2400 1108 Size: 1292 5366 PmeI GTTT'AAAC Cuts at: 3962 3962 Size: 6658 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4727 4759 6240 6272 4727 Size: 32 1481 32 5113 Fragments arranged by size: 5113 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2344 2829 2344 Size: 485 6173 PshAI GACnn'nnGTC Cuts at: 1613 2274 1613 Size: 661 5997 PsiI TTA'TAA Cuts at: 4228 4228 Size: 6658 PspOMI G'GGCC_C Cuts at: 2483 2483 Size: 6658 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3500 3532 3500 Size: 32 6626 PstI C_TGCA'G Cuts at: 830 2076 2649 3564 4010 830 Size: 1246 573 915 446 3478 Fragments arranged by size: 3478 1246 915 573 446 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6658 PvuII CAG'CTG Cuts at: 2923 3559 3565 2923 Size: 636 6 6016 Fragments arranged by size: 6016 636 6 SacI G_AGCT'C Cuts at: 3759 3759 Size: 6658 SacII CC_GC'GG Cuts at: 3330 3330 Size: 6658 SalI G'TCGA_C Cuts at: 1936 2414 4000 1936 Size: 478 1586 4594 Fragments arranged by size: 4594 1586 478 SanDI GG'GwC_CC Cuts at: 2829 2829 Size: 6658 SbfI CC_TGCA'GG Cuts at: 4010 4010 Size: 6658 ScaI AGT'ACT Cuts at: 4906 6618 4906 Size: 1712 4946 SexAI A'CCwGG_T Cuts at: 2469 3902 2469 Size: 1433 5225 SfcI C'TryA_G Cuts at: 826 1045 2072 2645 3560 4006 5141 5822 6013 Size: 219 1027 573 915 446 1135 681 191 Cuts at: 6013 826 Size: 1471 Fragments arranged by size: 1471 1135 1027 915 681 573 446 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6658 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6658 SmaI CCC'GGG Cuts at: 1872 2012 3985 1872 Size: 140 1973 4545 Fragments arranged by size: 4545 1973 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 2843 4762 5663 5925 820 Size: 197 926 900 1919 901 262 1553 Fragments arranged by size: 1919 1553 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 4449 493 Size: 3956 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6658 SphI G_CATG'C Cuts at: 1132 4016 1132 Size: 2884 3774 SspI AAT'ATT Cuts at: 5 52 4582 5 Size: 47 4530 2081 Fragments arranged by size: 4530 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6658 StyI C'CwwG_G Cuts at: 513 1065 2004 2592 3420 4097 513 Size: 552 939 588 828 677 3074 Fragments arranged by size: 3074 939 828 677 588 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 2392 2689 3587 4685 4702 4855 Size: 1255 707 105 297 898 1098 17 153 Cuts at: 4855 5040 5485 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1098 898 707 445 297 185 153 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2656 2664 4904 Size: 274 80 33 51 1443 679 8 2240 Cuts at: 4904 6616 96 Size: 1712 138 Fragments arranged by size: 2240 1712 1443 679 274 138 80 51 33 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2362 2394 3401 3433 6618 6650 2362 Size: 32 1007 32 3185 32 2370 Fragments arranged by size: 3185 2370 1007 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6658 XbaI T'CTAG_A Cuts at: 3994 3994 Size: 6658 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6658 XmaI C'CCGG_G Cuts at: 1870 2010 3983 1870 Size: 140 1973 4545 Fragments arranged by size: 4545 1973 140 XmnI GAAnn'nnTTC Cuts at: 3029 3083 3110 4787 3029 Size: 54 27 1677 4900 Fragments arranged by size: 4900 1677 54 27 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2937 4466 276 Size: 53 83 186 936 1403 1529 2468 Fragments arranged by size: 2468 1529 1403 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SapI SfiI SfoI SrfI SwaI XcmI