(Linear) (Six-base) MAPSORT of: KIEE4549.seq Check: 2620 from: 1 to: 1953 KIEE4549.orf With 182 enzymes: SgfI * March 22, 2012 18:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1272 1953 Size: 1272 681 AatII G_ACGT'C Cuts at: 0 934 1953 Size: 934 1019 AccI GT'mk_AC Cuts at: 0 410 1953 Size: 410 1543 Acc65I G'GTAC_C Cuts at: 0 188 1953 Size: 188 1765 AclI AA'CG_TT Cuts at: 0 1259 1953 Size: 1259 694 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1694 1953 Size: 1694 259 AflII C'TTAA_G Cuts at: 0 838 1953 Size: 838 1115 AleI CACnn'nnGTG Cuts at: 0 352 706 1953 Size: 352 354 1247 Fragments arranged by size: 1247 354 352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 938 1073 1953 Size: 938 135 880 Fragments arranged by size: 938 880 135 AlwNI CAG_nnn'CTG Cuts at: 0 342 860 1953 Size: 342 518 1093 Fragments arranged by size: 1093 518 342 ApaI G_GGCC'C Cuts at: 0 482 1953 Size: 482 1471 ApoI r'AATT_y Cuts at: 0 1761 1953 Size: 1761 192 AvaI C'yCGr_G Cuts at: 0 5 34 1953 Size: 5 29 1919 Fragments arranged by size: 1919 29 5 BamHI G'GATC_C Cuts at: 0 974 1653 1953 Size: 974 679 300 Fragments arranged by size: 974 679 300 BanI G'GyrC_C Cuts at: 0 188 843 1129 1535 1953 Size: 188 655 286 406 418 Fragments arranged by size: 655 418 406 286 188 BanII G_rGCy'C Cuts at: 0 100 482 1479 1754 1953 Size: 100 382 997 275 199 Fragments arranged by size: 997 382 275 199 100 BbvCI CC'TCA_GC Cuts at: 0 361 1953 Size: 361 1592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 871 905 1782 1816 1953 Size: 871 34 877 34 137 Fragments arranged by size: 877 871 137 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 593 1913 1953 Size: 593 1320 40 Fragments arranged by size: 1320 593 40 BglI GCCn_nnn'nGGC Cuts at: 0 28 492 1953 Size: 28 464 1461 Fragments arranged by size: 1461 464 28 BglII A'GATC_T Cuts at: 0 740 1953 Size: 740 1213 Bme1580I G_kGCm'C Cuts at: 0 378 482 1538 1953 Size: 378 104 1056 415 Fragments arranged by size: 1056 415 378 104 BmrI ACTGGGnnnn_n' Cuts at: 0 830 946 1563 1953 Size: 830 116 617 390 Fragments arranged by size: 830 617 390 116 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 225 1561 1953 Size: 153 72 1336 392 Fragments arranged by size: 1336 392 153 72 Bpu10I CC'TnA_GC Cuts at: 0 361 1953 Size: 361 1592 BsaAI yAC'GTr Cuts at: 0 1740 1953 Size: 1740 213 BsaBI GATnn'nnATC Cuts at: 0 1396 1953 Size: 1396 557 BsaHI Gr'CG_yC Cuts at: 0 165 270 931 1953 Size: 165 105 661 1022 Fragments arranged by size: 1022 661 165 105 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 85 115 172 202 1953 Size: 85 30 57 30 1751 Fragments arranged by size: 1751 85 57 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 193 1953 Size: 193 1760 BseYI C'CCAG_C Cuts at: 0 60 290 1803 1953 Size: 60 230 1513 150 Fragments arranged by size: 1513 230 150 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 908 934 1871 1953 Size: 908 26 937 82 Fragments arranged by size: 937 908 82 26 BsiEI CG_ry'CG Cuts at: 0 199 1328 1953 Size: 199 1129 625 Fragments arranged by size: 1129 625 199 BsiHKAI G_wGCw'C Cuts at: 0 1754 1953 Size: 1754 199 Bsp1286I G_dGCh'C Cuts at: 0 100 378 482 1479 1538 1754 1953 Size: 100 278 104 997 59 216 199 Fragments arranged by size: 997 278 216 199 104 100 59 BspHI T'CATG_A Cuts at: 0 1775 1953 Size: 1775 178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 350 1121 1272 1953 Size: 350 771 151 681 Fragments arranged by size: 771 681 350 151 BsrFI r'CCGG_y Cuts at: 0 191 1953 Size: 191 1762 BsrGI T'GTAC_A Cuts at: 0 659 1953 Size: 659 1294 BssHII G'CGCG_C Cuts at: 0 56 1953 Size: 56 1897 BstXI CCAn_nnnn'nTGG Cuts at: 0 708 1416 1953 Size: 708 708 537 Fragments arranged by size: 708 708 537 BstYI r'GATC_y Cuts at: 0 740 974 1653 1820 1953 Size: 740 234 679 167 133 Fragments arranged by size: 740 679 234 167 133 BtgI C'CryG_G Cuts at: 0 221 1035 1322 1953 Size: 221 814 287 631 Fragments arranged by size: 814 631 287 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 342 1953 Size: 342 1611 BtsI GCAGTG_nn' Cuts at: 0 190 378 1953 Size: 190 188 1575 Fragments arranged by size: 1575 190 188 ClaI AT'CG_AT Cuts at: 0 683 1211 1953 Size: 683 528 742 Fragments arranged by size: 742 683 528 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1554 1589 1953 Size: 1554 35 364 Fragments arranged by size: 1554 364 35 DrdI GACnn_nn'nnGTC Cuts at: 0 694 1785 1953 Size: 694 1091 168 Fragments arranged by size: 1091 694 168 EaeI y'GGCC_r Cuts at: 0 196 865 1087 1343 1562 1953 Size: 196 669 222 256 219 391 Fragments arranged by size: 669 391 256 222 219 196 EagI C'GGCC_G Cuts at: 0 196 1953 Size: 196 1757 EarI CTCTTCn'nnn_ Cuts at: 0 1670 1953 Size: 1670 283 EcoICRI GAG'CTC Cuts at: 0 1752 1953 Size: 1752 201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 225 1561 1694 1953 Size: 153 72 1336 133 259 Fragments arranged by size: 1336 259 153 133 72 EcoNI CCTnn'n_nnAGG Cuts at: 0 469 1953 Size: 469 1484 EcoO109I rG'GnC_Cy Cuts at: 0 214 339 478 479 824 940 1953 Size: 214 125 139 1 345 116 1013 Fragments arranged by size: 1013 345 214 139 125 116 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 612 799 1839 1953 Size: 612 187 1040 114 Fragments arranged by size: 1040 612 187 114 FspI TGC'GCA Cuts at: 0 346 1953 Size: 346 1607 HaeII r_GCGC'y Cuts at: 0 34 1240 1953 Size: 34 1206 713 Fragments arranged by size: 1206 713 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 85 117 1342 1374 1812 1844 1953 Size: 85 32 1225 32 438 32 109 Fragments arranged by size: 1225 438 109 85 32 32 32 HincII GTy'rAC Cuts at: 0 411 1044 1953 Size: 411 633 909 Fragments arranged by size: 909 633 411 KpnI G_GTAC'C Cuts at: 0 192 1953 Size: 192 1761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 752 1725 1953 Size: 752 973 228 Fragments arranged by size: 973 752 228 MscI TGG'CCA Cuts at: 0 1345 1564 1953 Size: 1345 219 389 Fragments arranged by size: 1345 389 219 MslI CAynn'nnrTG Cuts at: 0 352 383 706 1055 1282 1953 Size: 352 31 323 349 227 671 Fragments arranged by size: 671 352 349 323 227 31 MspA1I CmG'CkG Cuts at: 0 201 645 918 1092 1324 1554 1560 1953 Size: 201 444 273 174 232 230 6 393 Fragments arranged by size: 444 393 273 232 230 201 174 6 NotI GC'GGCC_GC Cuts at: 0 196 1953 Size: 196 1757 NspI r_CATG'y Cuts at: 0 231 1281 1953 Size: 231 1050 672 Fragments arranged by size: 1050 672 231 PasI CC'CwG_GG Cuts at: 0 828 1953 Size: 828 1125 PflMI CCAn_nnn'nTGG Cuts at: 0 395 1953 Size: 395 1558 PpuMI rG'GwC_Cy Cuts at: 0 339 824 1953 Size: 339 485 1129 Fragments arranged by size: 1129 485 339 PshAI GACnn'nnGTC Cuts at: 0 269 1953 Size: 269 1684 PspOMI G'GGCC_C Cuts at: 0 478 1953 Size: 478 1475 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1495 1527 1953 Size: 1495 32 426 Fragments arranged by size: 1495 426 32 PstI C_TGCA'G Cuts at: 0 71 644 1559 1953 Size: 71 573 915 394 Fragments arranged by size: 915 573 394 71 PvuII CAG'CTG Cuts at: 0 918 1554 1560 1953 Size: 918 636 6 393 Fragments arranged by size: 918 636 393 6 SacI G_AGCT'C Cuts at: 0 1754 1953 Size: 1754 199 SacII CC_GC'GG Cuts at: 0 1325 1953 Size: 1325 628 SalI G'TCGA_C Cuts at: 0 409 1953 Size: 409 1544 SanDI GG'GwC_CC Cuts at: 0 824 1953 Size: 824 1129 SexAI A'CCwGG_T Cuts at: 0 464 1897 1953 Size: 464 1433 56 Fragments arranged by size: 1433 464 56 SfcI C'TryA_G Cuts at: 0 67 640 1555 1953 Size: 67 573 915 398 Fragments arranged by size: 915 573 398 67 SmaI CCC'GGG Cuts at: 0 7 1953 Size: 7 1946 SmlI C'TyrA_G Cuts at: 0 838 1953 Size: 838 1115 StyI C'CwwG_G Cuts at: 0 587 1415 1953 Size: 587 828 538 Fragments arranged by size: 828 587 538 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 282 387 684 1582 1953 Size: 282 105 297 898 371 Fragments arranged by size: 898 371 297 282 105 TatI w'GTAC_w Cuts at: 0 651 659 1953 Size: 651 8 1294 Fragments arranged by size: 1294 651 8 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 357 389 1396 1428 1953 Size: 357 32 1007 32 525 Fragments arranged by size: 1007 525 357 32 32 XmaI C'CCGG_G Cuts at: 0 5 1953 Size: 5 1948 XmnI GAAnn'nnTTC Cuts at: 0 1024 1078 1105 1953 Size: 1024 54 27 848 Fragments arranged by size: 1024 848 54 27 ZraI GAC'GTC Cuts at: 0 932 1953 Size: 932 1021 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbvCI BcgI BciVI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI BsrGI BssHII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NotI NspI PasI PflMI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SacII SalI SanDI SexAI SfcI SmaI SmlI StyI TaqII TatI TstI XmaI XmnI ZraI Enzymes that do not cut: AfeI AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BclI BfrBI BlpI BmgBI BmtI BplI BpuEI BsaI BsaWI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I DraI DraIII EciI EcoRI EcoRV FseI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PvuI RsrII SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI