################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI ApaI AscI AseI AsnI Asp718I AspI AvrII BbeI BcgI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsiWI BsmBI BspHI BspLU11I BssHII Bst1107I BstAPI BstZ17I CelII CpoI DraI EagI EclXI Eco52I EcoRI EcoT22I FseI Hin1I HpaI KasI KpnI KspI MamI MluI NarI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SbfI ScaI SfiI SfoI SgrAI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 2744 TCCGGA T^CCGGA Alw44I 1650 GTGCAC G^TGCAC AocI 449 CCTCAGG CC^T[ACGT]AGG Aor51HI 3607 AGCGCT AGC^GCT ApaLI 1650 GTGCAC G^TGCAC BbrPI 3487 CACGTG CAC^GTG BciVI 149 GTATCC GTATCC(6/5) BclI 2756 TGATCA T^GATCA BsaI 3289 GGTCTC GGTCTC(1/5) BseAI 2744 TCCGGA T^CCGGA BsiEI 1489 CGACCG CG[GA][CT]^CG BspEI 2744 TCCGGA T^CCGGA BsrBI 3184 GAGCGG CCGCTC(-3/-3) BsrDI 2658 CATTGC GCAATG(2/0) BssSI 2953 CACGAG CACGAG(-5/-1) BstEII 2400 GGTCACC G^GT[ACGT]ACC BstPI 2400 GGTCACC G^GT[ACGT]ACC BstXI 2774 CCACGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 449 CCTCAGG CC^T[ACGT]AGG DrdI 2056 GACCGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3607 AGCGCT AGC^GCT Eco81I 449 CCTCAGG CC^T[ACGT]AGG EcoO65I 2400 GGTCACC G^GT[ACGT]ACC EcoRV 1937 GATATC GAT^ATC FbaI 2756 TGATCA T^GATCA HaeII 3607 AGCGCT [GA]GCGC^[CT] HincII 747 GTCGAC GT[CT]^[GA]AC HindII 747 GTCGAC GT[CT]^[GA]AC HindIII 1453 AAGCTT A^AGCTT MroI 2744 TCCGGA T^CCGGA NaeI 2440 GCCGGC GCC^GGC NgoMIV 2440 GCCGGC G^CCGGC PflMI 2952 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3487 CACGTG CAC^GTG PmlI 3487 CACGTG CAC^GTG PshAI 668 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 747 GTCGAC G^TCGAC SapI 957 GCTCTTC GCTCTTC(1/4) SexAI 3533 ACCAGGT A^CC[AT]GGT SmaI 2576 CCCGGG CCC^GGG SnaBI 458 TACGTA TAC^GTA Van91I 2952 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2576 CCCGGG C^CCGGG XmaI 2576 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 747 GTCGAC GT^[AC][GT]AC 1168 GTAGAC AcsI 1476 GAATTT [GA]^AATT[CT] 1816 GAATTT AhdI 1902 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3532 GACCAGGTGTC AlwNI 3196 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 3412 CAGCTGCTG ApoI 1476 GAATTT [GA]^AATT[CT] 1816 GAATTT Asp700I 1283 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2221 GAAAAGGTTC AspEI 1902 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3532 GACCAGGTGTC AviII 3090 TGCGCA TGC^GCA 3203 TGCGCA BamHI 1094 GGATCC G^GATCC 1857 GGATCC BsaAI 458 TACGTA [CT]AC^GT[GA] 3487 CACGTG BsaWI 2744 TCCGGA [AT]^CCGG[AT] 2968 ACCGGA Bsp1407I 1217 TGTACA T^GTACA 2339 TGTACA BspDI 1267 ATCGAT AT^CGAT 3298 ATCGAT BspMI 1654 ACCTGC ACCTGC(4/8) 2528 ACCTGC BsrFI 2440 GCCGGC [GA]^CCGG[CT] 3407 ACCGGC BsrGI 1217 TGTACA T^GTACA 2339 TGTACA BstBI 961 TTCGAA TT^CGAA 2310 TTCGAA Cfr10I 2440 GCCGGC [GA]^CCGG[CT] 3407 ACCGGC ClaI 1267 ATCGAT AT^CGAT 3298 ATCGAT DraIII 1372 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2953 CACGAGGTG Eam1105I 1902 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3532 GACCAGGTGTC FspI 3090 TGCGCA TGC^GCA 3203 TGCGCA MfeI 632 CAATTG C^AATTG 3542 CAATTG MunI 632 CAATTG C^AATTG 3542 CAATTG NcoI 660 CCATGG C^CATGG 3551 CCATGG NspV 961 TTCGAA TT^CGAA 2310 TTCGAA SacI 2425 GAGCTC GAGCT^C 2614 GAGCTC SfcI 144 CTACAG C^T[GA][CT]AG 2317 CTACAG SfuI 961 TTCGAA TT^CGAA 2310 TTCGAA SmlI 625 CTTGAG C^T[CT][GA]AG 952 CTCAAG SspBI 1217 TGTACA T^GTACA 2339 TGTACA XmnI 1283 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2221 GAAAAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII