(Linear) (Six-base) MAPSORT of: KIEE4594.seq Check: 8688 from: 1 to: 3705 KIEE4594.orf With 182 enzymes: SgfI * March 22, 2012 18:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1663 3705 Size: 1663 2042 AccI GT'mk_AC Cuts at: 0 748 1169 3705 Size: 748 421 2536 Fragments arranged by size: 2536 748 421 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1005 1280 2052 3705 Size: 1005 275 772 1653 Fragments arranged by size: 1653 1005 772 275 AfeI AGC'GCT Cuts at: 0 3609 3705 Size: 3609 96 AhdI GACnn_n'nnGTC Cuts at: 0 1907 3537 3705 Size: 1907 1630 168 Fragments arranged by size: 1907 1630 168 AleI CACnn'nnGTG Cuts at: 0 370 3705 Size: 370 3335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 137 2545 3705 Size: 137 2408 1160 Fragments arranged by size: 2408 1160 137 AlwNI CAG_nnn'CTG Cuts at: 0 3201 3417 3705 Size: 3201 216 288 Fragments arranged by size: 3201 288 216 ApaLI G'TGCA_C Cuts at: 0 1650 3705 Size: 1650 2055 ApoI r'AATT_y Cuts at: 0 1476 1816 3705 Size: 1476 340 1889 Fragments arranged by size: 1889 1476 340 AvaI C'yCGr_G Cuts at: 0 2569 2576 3156 3329 3400 3705 Size: 2569 7 580 173 71 305 Fragments arranged by size: 2569 580 305 173 71 7 BamHI G'GATC_C Cuts at: 0 1094 1857 3705 Size: 1094 763 1848 Fragments arranged by size: 1848 1094 763 BanI G'GyrC_C Cuts at: 0 1104 1191 3547 3705 Size: 1104 87 2356 158 Fragments arranged by size: 2356 1104 158 87 BanII G_rGCy'C Cuts at: 0 660 2429 2576 2618 3705 Size: 660 1769 147 42 1087 Fragments arranged by size: 1769 1087 660 147 42 BbsI GAAGACnn'nnnn_ Cuts at: 0 253 1011 1952 2058 2060 3705 Size: 253 758 941 106 2 1645 Fragments arranged by size: 1645 941 758 253 106 2 BbvCI CC'TCA_GC Cuts at: 0 1225 2207 2513 2836 3705 Size: 1225 982 306 323 869 Fragments arranged by size: 1225 982 869 323 306 BciVI GTATCCnnnnn_n' Cuts at: 0 160 3705 Size: 160 3545 BclI T'GATC_A Cuts at: 0 2756 3705 Size: 2756 949 Bme1580I G_kGCm'C Cuts at: 0 1654 2175 3705 Size: 1654 521 1530 Fragments arranged by size: 1654 1530 521 BmgBI CAC'GTC Cuts at: 0 2271 3143 3705 Size: 2271 872 562 Fragments arranged by size: 2271 872 562 BmrI ACTGGGnnnn_n' Cuts at: 0 1560 3705 Size: 1560 2145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 280 3705 Size: 280 3425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 382 844 1084 1680 3259 3583 3705 Size: 250 132 462 240 596 1579 324 122 Fragments arranged by size: 1579 596 462 324 250 240 132 122 Bpu10I CC'TnA_GC Cuts at: 0 1225 1627 2207 2513 2802 2836 3705 Size: 1225 402 580 306 289 34 869 Fragments arranged by size: 1225 869 580 402 306 289 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 646 937 3705 Size: 646 291 2768 Fragments arranged by size: 2768 646 291 BsaI GGTCTCn'nnnn_ Cuts at: 0 3295 3705 Size: 3295 410 BsaAI yAC'GTr Cuts at: 0 460 3489 3705 Size: 460 3029 216 Fragments arranged by size: 3029 460 216 BsaWI w'CCGG_w Cuts at: 0 2744 2968 3705 Size: 2744 224 737 Fragments arranged by size: 2744 737 224 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1778 1808 3277 3307 3705 Size: 1778 30 1469 30 398 Fragments arranged by size: 1778 1469 398 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1799 2024 2195 2626 2913 3003 3024 3705 Size: 1799 225 171 431 287 90 21 681 Fragments arranged by size: 1799 681 431 287 225 171 90 21 BseYI C'CCAG_C Cuts at: 0 2365 2369 2716 3176 3705 Size: 2365 4 347 460 529 Fragments arranged by size: 2365 529 460 347 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 319 2505 2848 3523 3705 Size: 73 246 2186 343 675 182 Fragments arranged by size: 2186 675 343 246 182 73 BsiEI CG_ry'CG Cuts at: 0 1492 3705 Size: 1492 2213 BsiHKAI G_wGCw'C Cuts at: 0 322 710 1654 1748 2429 2618 3705 Size: 322 388 944 94 681 189 1087 Fragments arranged by size: 1087 944 681 388 322 189 94 BsmI GAATG_Cn' Cuts at: 0 195 597 3093 3705 Size: 195 402 2496 612 Fragments arranged by size: 2496 612 402 195 Bsp1286I G_dGCh'C Cuts at: 0 322 660 710 1654 1748 2175 2429 2576 Size: 322 338 50 944 94 427 254 147 Cuts at: 2576 2618 3705 Size: 42 1087 Fragments arranged by size: 1087 944 427 338 322 254 147 94 50 42 BspEI T'CCGG_A Cuts at: 0 2744 3705 Size: 2744 961 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1663 2537 3705 Size: 1663 874 1168 Fragments arranged by size: 1663 1168 874 BsrBI CCG'CTC Cuts at: 0 3186 3705 Size: 3186 519 BsrDI GCAATG_nn' Cuts at: 0 2657 3705 Size: 2657 1048 BsrFI r'CCGG_y Cuts at: 0 2440 3407 3705 Size: 2440 967 298 Fragments arranged by size: 2440 967 298 BsrGI T'GTAC_A Cuts at: 0 1217 2339 3705 Size: 1217 1122 1366 Fragments arranged by size: 1366 1217 1122 BssSI C'ACGA_G Cuts at: 0 2953 3705 Size: 2953 752 BstBI TT'CG_AA Cuts at: 0 962 2311 3705 Size: 962 1349 1394 Fragments arranged by size: 1394 1349 962 BstEII G'GTnAC_C Cuts at: 0 2400 3705 Size: 2400 1305 BstXI CCAn_nnnn'nTGG Cuts at: 0 2781 3705 Size: 2781 924 BstYI r'GATC_y Cuts at: 0 1094 1857 3269 3705 Size: 1094 763 1412 436 Fragments arranged by size: 1412 1094 763 436 Bsu36I CC'TnA_GG Cuts at: 0 450 3705 Size: 450 3255 BtgI C'CryG_G Cuts at: 0 660 2555 2774 3242 3285 3551 3705 Size: 660 1895 219 468 43 266 154 Fragments arranged by size: 1895 660 468 266 219 154 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 215 3705 Size: 215 3490 BtsI GCAGTG_nn' Cuts at: 0 708 812 3093 3659 3705 Size: 708 104 2281 566 46 Fragments arranged by size: 2281 708 566 104 46 ClaI AT'CG_AT Cuts at: 0 1268 3299 3705 Size: 1268 2031 406 Fragments arranged by size: 2031 1268 406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3635 3670 3705 Size: 3635 35 35 Fragments arranged by size: 3635 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1377 2958 3705 Size: 1377 1581 747 Fragments arranged by size: 1581 1377 747 DrdI GACnn_nn'nnGTC Cuts at: 0 2062 3705 Size: 2062 1643 EaeI y'GGCC_r Cuts at: 0 155 287 611 2693 2771 3509 3705 Size: 155 132 324 2082 78 738 196 Fragments arranged by size: 2082 738 324 196 155 132 78 EarI CTCTTCn'nnn_ Cuts at: 0 424 964 1139 2804 3705 Size: 424 540 175 1665 901 Fragments arranged by size: 1665 901 540 424 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1087 3705 Size: 1087 2618 EcoICRI GAG'CTC Cuts at: 0 2427 2616 3705 Size: 2427 189 1089 Fragments arranged by size: 2427 1089 189 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 382 844 1005 1084 1280 1680 2052 Size: 250 132 462 161 79 196 400 372 Cuts at: 2052 3259 3583 3705 Size: 1207 324 122 Fragments arranged by size: 1207 462 400 372 324 250 196 161 132 122 79 EcoNI CCTnn'n_nnAGG Cuts at: 0 1659 2221 2512 2671 3705 Size: 1659 562 291 159 1034 Fragments arranged by size: 1659 1034 562 291 159 EcoO109I rG'GnC_Cy Cuts at: 0 165 918 1059 2021 2362 2505 3237 3705 Size: 165 753 141 962 341 143 732 468 Fragments arranged by size: 962 753 732 468 341 165 143 141 EcoRV GAT'ATC Cuts at: 0 1939 3705 Size: 1939 1766 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1993 3705 Size: 1993 1712 FspI TGC'GCA Cuts at: 0 3092 3205 3705 Size: 3092 113 500 Fragments arranged by size: 3092 500 113 FspAI rTGC'GCAy Cuts at: 0 3092 3705 Size: 3092 613 HaeII r_GCGC'y Cuts at: 0 3611 3705 Size: 3611 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 243 275 397 429 1422 1454 1667 1699 Size: 243 32 122 32 993 32 213 32 Cuts at: 1699 1893 1925 2010 2042 3155 3187 3498 3523 Size: 194 32 85 32 1113 32 311 25 Cuts at: 3523 3530 3555 3705 Size: 7 25 150 Fragments arranged by size: 1113 993 311 243 213 194 150 122 85 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 0 749 3705 Size: 749 2956 HindIII A'AGCT_T Cuts at: 0 1453 3705 Size: 1453 2252 MfeI C'AATT_G Cuts at: 0 632 3542 3705 Size: 632 2910 163 Fragments arranged by size: 2910 632 163 MscI TGG'CCA Cuts at: 0 289 613 2695 2773 3511 3705 Size: 289 324 2082 78 738 194 Fragments arranged by size: 2082 738 324 289 194 78 MslI CAynn'nnrTG Cuts at: 0 370 1445 1505 2764 3433 3460 3487 3705 Size: 370 1075 60 1259 669 27 27 218 Fragments arranged by size: 1259 1075 669 370 218 60 27 27 MspA1I CmG'CkG Cuts at: 0 1258 2369 3414 3519 3705 Size: 1258 1111 1045 105 186 Fragments arranged by size: 1258 1111 1045 186 105 NaeI GCC'GGC Cuts at: 0 2442 3705 Size: 2442 1263 NcoI C'CATG_G Cuts at: 0 660 3551 3705 Size: 660 2891 154 Fragments arranged by size: 2891 660 154 NgoMIV G'CCGG_C Cuts at: 0 2440 3705 Size: 2440 1265 PflMI CCAn_nnn'nTGG Cuts at: 0 2958 3705 Size: 2958 747 PfoI T'CCnGG_A Cuts at: 0 581 3705 Size: 581 3124 PmlI CAC'GTG Cuts at: 0 3489 3705 Size: 3489 216 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 941 973 3705 Size: 941 32 2732 Fragments arranged by size: 2732 941 32 PpuMI rG'GwC_Cy Cuts at: 0 918 2021 2362 2505 3237 3705 Size: 918 1103 341 143 732 468 Fragments arranged by size: 1103 918 732 468 341 143 PshAI GACnn'nnGTC Cuts at: 0 672 3705 Size: 672 3033 PsiI TTA'TAA Cuts at: 0 902 1006 3705 Size: 902 104 2699 Fragments arranged by size: 2699 902 104 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1160 1192 3705 Size: 1160 32 2513 Fragments arranged by size: 2513 1160 32 PvuII CAG'CTG Cuts at: 0 2369 3414 3519 3705 Size: 2369 1045 105 186 Fragments arranged by size: 2369 1045 186 105 SacI G_AGCT'C Cuts at: 0 2429 2618 3705 Size: 2429 189 1087 Fragments arranged by size: 2429 1087 189 SalI G'TCGA_C Cuts at: 0 747 3705 Size: 747 2958 SanDI GG'GwC_CC Cuts at: 0 2362 3237 3705 Size: 2362 875 468 Fragments arranged by size: 2362 875 468 SapI GCTCTTCn'nnn_ Cuts at: 0 964 3705 Size: 964 2741 SexAI A'CCwGG_T Cuts at: 0 3533 3705 Size: 3533 172 SfcI C'TryA_G Cuts at: 0 144 2317 3705 Size: 144 2173 1388 Fragments arranged by size: 2173 1388 144 SmaI CCC'GGG Cuts at: 0 2578 3705 Size: 2578 1127 SmlI C'TyrA_G Cuts at: 0 625 952 3705 Size: 625 327 2753 Fragments arranged by size: 2753 625 327 SnaBI TAC'GTA Cuts at: 0 460 3705 Size: 460 3245 StuI AGG'CCT Cuts at: 0 220 2483 2518 3705 Size: 220 2263 35 1187 Fragments arranged by size: 2263 1187 220 35 StyI C'CwwG_G Cuts at: 0 215 323 614 660 1512 3551 3705 Size: 215 108 291 46 852 2039 154 Fragments arranged by size: 2039 852 291 215 154 108 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 117 1187 3705 Size: 117 1070 2518 Fragments arranged by size: 2518 1070 117 TatI w'GTAC_w Cuts at: 0 344 1148 1217 1317 2339 3705 Size: 344 804 69 100 1022 1366 Fragments arranged by size: 1366 1022 804 344 100 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1673 1705 3277 3309 3705 Size: 1673 32 1572 32 396 Fragments arranged by size: 1673 1572 396 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 868 2724 2875 2955 3541 3558 3705 Size: 868 1856 151 80 586 17 147 Fragments arranged by size: 1856 868 586 151 147 80 17 XmaI C'CCGG_G Cuts at: 0 2576 3705 Size: 2576 1129 XmnI GAAnn'nnTTC Cuts at: 0 1287 2225 3705 Size: 1287 938 1480 Fragments arranged by size: 1480 1287 938 Enzymes that do cut: AarI AccI AcuI AfeI AhdI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII MfeI MscI MslI MspA1I NaeI NcoI NgoMIV PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PsrI PvuII SacI SalI SanDI SapI SexAI SfcI SmaI SmlI SnaBI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI BfrBI BglI BglII BlpI BmtI BsaBI BsaHI BsiWI BsmBI BspHI BssHII BstAPI BstZ17I DraI EagI EcoRI FseI HpaI KasI KpnI MluI MmeI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PspOMI PspXI PstI PvuI RsrII SacII SbfI ScaI SfiI SfoI SgfI SgrAI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI ZraI