################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BbeI BcgI BciVI BclI BfrI BlnI BsaBI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrGI BssHII BstAPI Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AcyI 1982 GACGCC G[GA]^CG[CT]C AflIII 1796 ACATGT A^C[GA][CT]GT BbvCI 1009 CCTCAGC CCTCAGC(-5/-2) BglI 1253 GCCGCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 1982 GACGCC G[GA]^CG[CT]C BspLU11I 1796 ACATGT A^CATGT BsrFI 2012 GCCGGC [GA]^CCGG[CT] BssSI 1451 CTCGTG CACGAG(-5/-1) Bst1107I 1767 GTATAC GTA^TAC BstBI 2108 TTCGAA TT^CGAA BstXI 331 CCACTTGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1767 GTATAC GTA^TAC Cfr10I 2012 GCCGGC [GA]^CCGG[CT] DrdI 126 GACAGATTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 867 GAATTC G^AATTC EcoT14I 683 CCATGG C^C[AT][AT]GG HgaI 1982 GACGC GACGC(5/10) Hin1I 1982 GACGCC G[GA]^CG[CT]C HincII 1810 GTCAAC GT[CT]^[GA]AC HindII 1810 GTCAAC GT[CT]^[GA]AC HindIII 186 AAGCTT A^AGCTT NaeI 2012 GCCGGC GCC^GGC NcoI 683 CCATGG C^CATGG NgoMIV 2012 GCCGGC G^CCGGC NspV 2108 TTCGAA TT^CGAA PflMI 1077 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SapI 708 GCTCTTC GCTCTTC(1/4) SfuI 2108 TTCGAA TT^CGAA SgrAI 2011 CGCCGGCG C[GA]^CCGG[CT]G SspI 689 AATATT AAT^ATT StyI 683 CCATGG C^C[AT][AT]GG Van91I 1077 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 359 CCATCATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 352 GTCTAC GT^[AC][GT]AC 1767 GTATAC AccII 21 CGCG CG^CG 23 CGCG AcsI 867 GAATTC [GA]^AATT[CT] 1623 GAATTT Alw44I 763 GTGCAC G^TGCAC 1207 GTGCAC AlwNI 1660 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 1915 CAGCCTCTG ApaI 79 GGGCCC GGGCC^C 1944 GGGCCC ApaLI 763 GTGCAC G^TGCAC 1207 GTGCAC ApoI 867 GAATTC [GA]^AATT[CT] 1623 GAATTT BbrPI 580 CACGTG CAC^GTG 1228 CACGTG BbsI 1636 GAAGAC GAAGAC(2/6) 1588 GTCTTC BglII 617 AGATCT A^GATCT 1391 AGATCT BlpI 188 GCTTAGC GC^T[ACGT]AGC 252 GCTCAGC Bpu1102I 188 GCTTAGC GC^T[ACGT]AGC 252 GCTCAGC BpuAI 1636 GAAGAC GAAGAC(2/6) 1588 GTCTTC BsaAI 580 CACGTG [CT]AC^GT[GA] 1228 CACGTG BsaI 2175 GAGACC GGTCTC(1/5) 1410 GGTCTC BsaWI 266 ACCGGA [AT]^CCGG[AT] 404 ACCGGA BseRI 739 GAGGAG GAGGAG(10/8) 1038 GAGGAG BsgI 1612 CTGCAC GTGCAG(16/14) 1465 GTGCAG BsmI 558 GAATGC GAATGC(1/-1) 573 GAATGC BsrBI 1215 CCGCTC CCGCTC(-3/-3) 244 GAGCGG BsrDI 636 CATTGC GCAATG(2/0) 1593 CATTGC BstEII 153 GGTGACC G^GT[ACGT]ACC 1478 GGTCACC BstPI 153 GGTGACC G^GT[ACGT]ACC 1478 GGTCACC BstUI 21 CGCG CG^CG 23 CGCG CelII 188 GCTTAGC GC^T[ACGT]AGC 252 GCTCAGC DsaI 609 CCGTGG C^C[GA][CT]GG 683 CCATGG EcoO65I 153 GGTGACC G^GT[ACGT]ACC 1478 GGTCACC MseI 1277 TTAA T^TAA 1861 TTAA MvnI 21 CGCG CG^CG 23 CGCG NspI 1325 ACATGC [GA]CATG^[CT] 1796 ACATGT PmaCI 580 CACGTG CAC^GTG 1228 CACGTG PmlI 580 CACGTG CAC^GTG 1228 CACGTG PstI 1666 CTGCAG CTGCA^G 1912 CTGCAG Tru9I 1277 TTAA T^TAA 1861 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII