(Circular) (Six-base) MAPSORT of: pFN21AE4575.seq Check: 3774 from: 1 to: 6979 pFN21AE4575 6979 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4789 278 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2358 3773 4322 1937 Size: 421 1415 549 4594 Fragments arranged by size: 4594 1415 549 421 Acc65I G'GTAC_C Cuts at: 4300 4300 Size: 6979 AclI AA'CG_TT Cuts at: 1563 5106 5479 1563 Size: 3543 373 3063 Fragments arranged by size: 3543 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2701 3356 5042 6426 6694 1632 Size: 1069 655 1686 1384 268 1917 Fragments arranged by size: 1917 1686 1384 1069 655 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6782 AflIII A'CryG_T Cuts at: 1203 3801 5878 6855 1203 Size: 2598 2077 977 1327 Fragments arranged by size: 2598 2077 1327 977 AgeI A'CCGG_T Cuts at: 5810 5810 Size: 6979 AhdI GACnn_n'nnGTC Cuts at: 5708 5708 Size: 6979 AleI CACnn'nnGTG Cuts at: 1166 2775 3587 1166 Size: 1609 812 4558 Fragments arranged by size: 4558 1609 812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3683 3765 1184 Size: 2499 82 4398 Fragments arranged by size: 4398 2499 82 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 2468 Size: 32 6947 AlwNI CAG_nnn'CTG Cuts at: 3670 3925 4722 6294 3670 Size: 255 797 1572 4355 Fragments arranged by size: 4355 1572 797 255 ApaI G_GGCC'C Cuts at: 2088 3953 2088 Size: 1865 5114 ApaLI G'TGCA_C Cuts at: 2768 3212 5036 6192 2768 Size: 444 1824 1156 3555 Fragments arranged by size: 3555 1824 1156 444 ApoI r'AATT_y Cuts at: 1493 2872 3628 4288 4514 4666 6775 1493 Size: 1379 756 660 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 756 660 226 152 AseI AT'TA_AT Cuts at: 160 1032 5533 160 Size: 872 4501 1606 Fragments arranged by size: 4501 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 AvaI C'yCGr_G Cuts at: 1870 1943 4304 1870 Size: 73 2361 4545 Fragments arranged by size: 4545 2361 73 BamHI G'GATC_C Cuts at: 4309 4676 4309 Size: 367 6612 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4300 5755 618 Size: 325 217 627 2513 1455 1842 Fragments arranged by size: 2513 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2259 3393 3953 4298 1391 Size: 697 171 1134 560 345 4072 Fragments arranged by size: 4072 1134 697 560 345 171 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3586 3648 928 Size: 971 1687 62 4259 Fragments arranged by size: 4259 1687 971 62 BbvCI CC'TCA_GC Cuts at: 3015 3015 Size: 6979 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5170 5204 1903 Size: 34 3233 34 3678 Fragments arranged by size: 3678 3233 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4871 6081 4871 Size: 1210 5769 BclI T'GATC_A Cuts at: 1548 1548 Size: 6979 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3264 5590 136 Size: 107 122 71 631 605 1592 2326 1525 Fragments arranged by size: 2326 1592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2622 3396 6974 2622 Size: 774 3578 2627 Fragments arranged by size: 3578 2627 774 BlpI GC'TnA_GC Cuts at: 2194 2258 4396 2194 Size: 64 2138 4777 Fragments arranged by size: 4777 2138 64 Bme1580I G_kGCm'C Cuts at: 1790 2088 2383 2592 2772 3216 3953 5040 6196 Size: 298 295 209 180 444 737 1087 1156 Cuts at: 6196 1790 Size: 2573 Fragments arranged by size: 2573 1156 1087 737 444 298 295 209 180 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6979 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2393 2680 2782 Size: 416 53 83 399 24 976 287 102 Cuts at: 2782 3015 3281 4710 5668 442 Size: 233 266 1429 958 1753 Fragments arranged by size: 1753 1429 976 958 416 399 287 266 233 102 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6979 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3140 3559 3857 3986 4163 5639 1239 Size: 1901 419 298 129 177 1476 2579 Fragments arranged by size: 2579 1901 1476 419 298 177 129 Bpu10I CC'TnA_GC Cuts at: 3015 3356 3954 3015 Size: 341 598 6040 Fragments arranged by size: 6040 598 341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2812 2987 4198 5104 5969 6267 2812 Size: 175 1211 906 865 298 3524 Fragments arranged by size: 3524 1211 906 865 298 175 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3421 4174 5642 882 Size: 617 1922 753 1468 2219 Fragments arranged by size: 2219 1922 1468 753 617 BsaAI yAC'GTr Cuts at: 493 1204 2587 3235 4770 493 Size: 711 1383 648 1535 2702 Fragments arranged by size: 2702 1535 1383 711 648 BsaBI GATnn'nnATC Cuts at: 6973 6973 Size: 6979 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3988 4786 5168 5803 Size: 53 83 186 936 2455 798 382 635 Cuts at: 5803 275 Size: 1451 Fragments arranged by size: 2455 1451 936 798 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2271 2409 5412 5810 6084 6231 6642 2271 Size: 138 3003 398 274 147 411 2608 Fragments arranged by size: 3003 2608 411 398 274 147 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 3206 3236 3984 Size: 30 165 30 1065 30 708 30 748 Cuts at: 3984 4014 1178 Size: 30 4143 Fragments arranged by size: 4143 1065 748 708 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2759 3058 1186 Size: 1573 299 5107 Fragments arranged by size: 5107 1573 299 BseYI C'CCAG_C Cuts at: 2039 2395 2877 3088 6182 6678 2039 Size: 356 482 211 3094 496 2340 Fragments arranged by size: 3094 2340 496 482 356 211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3491 3602 1163 Size: 573 1755 111 4540 Fragments arranged by size: 4540 1755 573 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4429 5190 6218 1540 Size: 275 186 2428 761 1028 2301 Fragments arranged by size: 2428 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2613 2772 3216 5040 5125 6196 2613 Size: 159 444 1824 85 1071 3396 Fragments arranged by size: 3396 1824 1071 444 159 85 BsmI GAATG_Cn' Cuts at: 2569 2584 4490 4583 2569 Size: 15 1906 93 4965 Fragments arranged by size: 4965 1906 93 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2259 2383 2592 2613 2772 3216 Size: 399 298 171 124 209 21 159 444 Cuts at: 3216 3393 3953 4298 5040 5125 6196 1391 Size: 177 560 345 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 560 444 399 345 298 209 177 171 159 124 85 21 BspEI T'CCGG_A Cuts at: 6642 6642 Size: 6979 BspHI T'CATG_A Cuts at: 4868 6598 4868 Size: 1730 5249 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4334 844 Size: 3490 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2251 3222 4866 1394 Size: 429 428 971 1644 3507 Fragments arranged by size: 3507 1644 971 429 428 BsrDI GCAATG_nn' Cuts at: 65 1248 2640 3597 5474 5648 65 Size: 1183 1392 957 1877 174 1396 Fragments arranged by size: 1877 1396 1392 1183 957 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4017 5623 5810 1700 Size: 110 2207 1606 187 2869 Fragments arranged by size: 2869 2207 1606 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6979 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6979 BssSI C'ACGA_G Cuts at: 3456 5039 6051 3456 Size: 1583 1012 4384 Fragments arranged by size: 4384 1583 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6979 BstBI TT'CG_AA Cuts at: 4114 5783 4114 Size: 1669 5310 BstEII G'GTnAC_C Cuts at: 1184 2158 3483 1184 Size: 974 1325 4680 Fragments arranged by size: 4680 1325 974 BstXI CCAn_nnnn'nTGG Cuts at: 2343 2343 Size: 6979 BstYI r'GATC_y Cuts at: 1972 2622 2831 3396 4309 4676 5061 5078 6530 Size: 650 209 565 913 367 385 17 1452 Cuts at: 6530 6616 6628 6974 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 913 650 565 385 367 346 209 86 17 12 BstZ17I GTA'TAC Cuts at: 3774 3774 Size: 6979 BtgI C'CryG_G Cuts at: 513 1065 2004 2614 2688 513 Size: 552 939 610 74 4804 Fragments arranged by size: 4804 939 610 552 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3325 487 Size: 683 56 2099 4141 Fragments arranged by size: 4141 2099 683 56 BtsI GCAGTG_nn' Cuts at: 2330 2613 2863 4495 5289 5309 2330 Size: 283 250 1632 794 20 4000 Fragments arranged by size: 4000 1632 794 283 250 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6979 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6944 DraI TTT'AAA Cuts at: 4283 4283 Size: 6979 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2137 5986 809 Size: 507 821 3849 1802 Fragments arranged by size: 3849 1802 821 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2617 2916 3061 Size: 54 1425 50 275 18 787 299 145 Cuts at: 3061 3256 4426 5315 6944 8 Size: 195 1170 889 1629 43 Fragments arranged by size: 1629 1425 1170 889 787 299 275 195 145 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4426 1537 Size: 275 2614 4090 Fragments arranged by size: 4090 2614 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2720 2936 3726 4909 1353 Size: 1367 216 790 1183 3423 Fragments arranged by size: 3423 1367 1183 790 216 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2164 5566 5940 6086 387 Size: 1064 138 575 3402 374 146 1280 Fragments arranged by size: 3402 1280 1064 575 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2701 3140 3356 3559 3857 3986 4163 Size: 393 1069 439 216 203 298 129 177 Cuts at: 4163 5042 5639 6426 6694 1239 Size: 879 597 787 268 1524 Fragments arranged by size: 1524 1069 879 787 597 439 393 298 268 216 203 177 129 EcoO109I rG'GnC_Cy Cuts at: 2084 3899 3949 3950 2084 Size: 1815 50 1 5113 Fragments arranged by size: 5113 1815 50 1 EcoRI G'AATT_C Cuts at: 2872 4288 4666 2872 Size: 1416 378 5185 Fragments arranged by size: 5185 1416 378 FspI TGC'GCA Cuts at: 5485 5485 Size: 6979 HaeII r_GCGC'y Cuts at: 1678 6126 1678 Size: 4448 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 5620 5652 5694 Size: 32 135 32 881 32 3135 32 42 Cuts at: 5694 5726 1373 Size: 32 2626 Fragments arranged by size: 3135 2626 881 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3817 4323 4569 669 Size: 988 281 1879 506 246 3079 Fragments arranged by size: 3079 1879 988 506 281 246 HindIII A'AGCT_T Cuts at: 748 2191 748 Size: 1443 5536 HpaI GTT'AAC Cuts at: 4569 4569 Size: 6979 KpnI G_GTAC'C Cuts at: 4304 4304 Size: 6979 MfeI C'AATT_G Cuts at: 4578 4578 Size: 6979 MluI A'CGCG_T Cuts at: 6855 6855 Size: 6979 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3909 4177 6093 6277 1305 Size: 2604 268 1916 184 2007 Fragments arranged by size: 2604 2007 1916 268 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2619 2918 3063 10 Size: 54 1425 343 787 299 145 3926 Fragments arranged by size: 3926 1425 787 343 299 145 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2366 2775 2894 3209 3587 4937 Size: 648 843 357 409 119 315 378 1350 Cuts at: 4937 5296 5455 518 Size: 359 159 2042 Fragments arranged by size: 2042 1350 843 648 409 378 359 357 315 159 119 MspA1I CmG'CkG Cuts at: 1606 2801 3088 3667 4150 4395 5072 6220 6465 Size: 1195 287 579 483 245 677 1148 245 Cuts at: 6465 1606 Size: 2120 Fragments arranged by size: 2120 1195 1148 677 579 483 287 245 245 NaeI GCC'GGC Cuts at: 4019 4019 Size: 6979 NcoI C'CATG_G Cuts at: 513 1065 2004 2688 513 Size: 552 939 684 4804 Fragments arranged by size: 4804 939 684 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6979 NgoMIV G'CCGG_C Cuts at: 4017 4017 Size: 6979 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6979 NotI GC'GGCC_GC Cuts at: 4426 4426 Size: 6979 NspI r_CATG'y Cuts at: 1132 1229 3334 3805 4337 5882 1132 Size: 97 2105 471 532 1545 2229 Fragments arranged by size: 2229 2105 1545 532 471 97 PasI CC'CwG_GG Cuts at: 1348 3157 3287 1348 Size: 1809 130 5040 Fragments arranged by size: 5040 1809 130 PciI A'CATG_T Cuts at: 3801 5878 3801 Size: 2077 4902 PflMI CCAn_nnn'nTGG Cuts at: 1108 3088 1108 Size: 1980 4999 PfoI T'CCnGG_A Cuts at: 2457 3117 4002 2457 Size: 660 885 5434 Fragments arranged by size: 5434 885 660 PmeI GTTT'AAAC Cuts at: 4283 4283 Size: 6979 PmlI CAC'GTG Cuts at: 2587 3235 2587 Size: 648 6331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2468 2500 5048 5080 6561 6593 2468 Size: 32 2548 32 1481 32 2854 Fragments arranged by size: 2854 2548 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6979 PsiI TTA'TAA Cuts at: 4549 4549 Size: 6979 PspOMI G'GGCC_C Cuts at: 2084 3949 2084 Size: 1865 5114 PstI C_TGCA'G Cuts at: 830 3675 3921 4331 830 Size: 2845 246 410 3478 Fragments arranged by size: 3478 2845 410 246 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6979 PvuII CAG'CTG Cuts at: 2801 3088 3667 4150 2801 Size: 287 579 483 5630 Fragments arranged by size: 5630 579 483 287 SalI G'TCGA_C Cuts at: 1936 4321 1936 Size: 2385 4594 SapI GCTCTTCn'nnn_ Cuts at: 2720 2720 Size: 6979 SbfI CC_TGCA'GG Cuts at: 4331 4331 Size: 6979 ScaI AGT'ACT Cuts at: 5227 6939 5227 Size: 1712 5267 SfcI C'TryA_G Cuts at: 826 1045 3671 3822 3917 4327 5462 6143 6334 Size: 219 2626 151 95 410 1135 681 191 Cuts at: 6334 826 Size: 1471 Fragments arranged by size: 2626 1471 1135 681 410 219 191 151 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6979 SgrAI Cr'CCGG_yG Cuts at: 1700 4017 1700 Size: 2317 4662 SmaI CCC'GGG Cuts at: 1872 4306 1872 Size: 2434 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2827 3002 4177 5083 5984 6246 Size: 197 926 884 175 1175 906 901 262 Cuts at: 6246 820 Size: 1553 Fragments arranged by size: 1553 1175 926 906 901 884 262 197 175 SnaBI TAC'GTA Cuts at: 493 4770 493 Size: 4277 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6979 SphI G_CATG'C Cuts at: 1132 4337 1132 Size: 3205 3774 SspI AAT'ATT Cuts at: 5 52 2696 4903 5 Size: 47 2644 2207 2081 Fragments arranged by size: 2644 2207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6979 StyI C'CwwG_G Cuts at: 513 1065 2004 2688 4418 513 Size: 552 939 684 1730 3074 Fragments arranged by size: 3074 1730 939 684 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3170 3496 5006 5023 5176 5361 5806 Size: 1255 1590 326 1510 17 153 185 445 Cuts at: 5806 325 Size: 1498 Fragments arranged by size: 1590 1510 1498 1255 445 326 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2053 2634 5225 Size: 274 80 33 51 1443 76 581 2591 Cuts at: 5225 6937 96 Size: 1712 138 Fragments arranged by size: 2591 1712 1443 581 274 138 80 76 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2436 3206 3238 6939 6971 2404 Size: 32 770 32 3701 32 2412 Fragments arranged by size: 3701 2412 770 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6979 XbaI T'CTAG_A Cuts at: 4315 4315 Size: 6979 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2371 2371 Size: 6979 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6979 XmaI C'CCGG_G Cuts at: 1870 4304 1870 Size: 2434 4545 XmnI GAAnn'nnTTC Cuts at: 5108 5108 Size: 6979 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4787 276 Size: 53 83 186 936 3253 2468 Fragments arranged by size: 3253 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI