(Circular) (Six-base) MAPSORT of: pFN21AE3589.seq Check: 497 from: 1 to: 5386 pFN21AE3589 5386 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3196 278 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2729 1937 Size: 792 4594 Acc65I G'GTAC_C Cuts at: 2707 2707 Size: 5386 AclI AA'CG_TT Cuts at: 1563 3513 3886 1563 Size: 1950 373 3063 Fragments arranged by size: 3063 1950 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2658 3449 4833 5101 1632 Size: 491 535 791 1384 268 1917 Fragments arranged by size: 1917 1384 791 535 491 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5189 AflIII A'CryG_T Cuts at: 1203 4285 5262 1203 Size: 3082 977 1327 Fragments arranged by size: 3082 1327 977 AgeI A'CCGG_T Cuts at: 4217 4217 Size: 5386 AhdI GACnn_n'nnGTC Cuts at: 4115 4115 Size: 5386 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5386 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5386 AlwNI CAG_nnn'CTG Cuts at: 3129 4701 3129 Size: 1572 3814 ApaI G_GGCC'C Cuts at: 2050 2050 Size: 5386 ApaLI G'TGCA_C Cuts at: 3443 4599 3443 Size: 1156 4230 ApoI r'AATT_y Cuts at: 1493 2426 2695 2921 3073 5182 1493 Size: 933 269 226 152 2109 1697 Fragments arranged by size: 2109 1697 933 269 226 152 AseI AT'TA_AT Cuts at: 160 1032 3940 160 Size: 872 2908 1606 Fragments arranged by size: 2908 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 AvaI C'yCGr_G Cuts at: 1870 1943 2050 2056 2711 1870 Size: 73 107 6 655 4545 Fragments arranged by size: 4545 655 107 73 6 BamHI G'GATC_C Cuts at: 2716 3083 2716 Size: 367 5019 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2707 4162 618 Size: 325 217 627 279 641 1455 1842 Fragments arranged by size: 1842 1455 641 627 325 279 217 BanII G_rGCy'C Cuts at: 1391 2050 2057 2705 1391 Size: 659 7 648 4072 Fragments arranged by size: 4072 659 648 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2541 928 Size: 971 642 3773 Fragments arranged by size: 3773 971 642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3577 3611 1903 Size: 34 1640 34 3678 Fragments arranged by size: 3678 1640 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3278 4488 3278 Size: 1210 4176 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 4897 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3997 136 Size: 107 122 71 631 605 337 1988 1525 Fragments arranged by size: 1988 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5381 5381 Size: 5386 BlpI GC'TnA_GC Cuts at: 2803 2803 Size: 5386 Bme1580I G_kGCm'C Cuts at: 1790 2050 2069 2153 2355 3447 4603 1790 Size: 260 19 84 202 1092 1156 2573 Fragments arranged by size: 2573 1156 1092 260 202 84 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5386 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2248 2475 3117 Size: 416 53 83 399 24 831 227 642 Cuts at: 3117 4075 442 Size: 958 1753 Fragments arranged by size: 1753 958 831 642 416 399 227 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2667 4046 1239 Size: 859 569 1379 2579 Fragments arranged by size: 2579 1379 859 569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3511 4376 4674 3511 Size: 865 298 4223 Fragments arranged by size: 4223 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4049 882 Size: 617 2550 2219 Fragments arranged by size: 2550 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3177 493 Size: 711 1973 2702 Fragments arranged by size: 2702 1973 711 BsaBI GATnn'nnATC Cuts at: 5380 5380 Size: 5386 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3193 3575 4210 Size: 53 83 186 936 479 1181 382 635 Cuts at: 4210 275 Size: 1451 Fragments arranged by size: 1451 1181 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2594 3819 4217 4491 4638 5049 2594 Size: 1225 398 274 147 411 2931 Fragments arranged by size: 2931 1225 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5161 Fragments arranged by size: 5161 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5386 BseYI C'CCAG_C Cuts at: 4589 5085 4589 Size: 496 4890 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4813 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2836 3597 4625 1540 Size: 275 186 835 761 1028 2301 Fragments arranged by size: 2301 1028 835 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3447 3532 4603 3447 Size: 85 1071 4230 Fragments arranged by size: 4230 1071 85 BsmI GAATG_Cn' Cuts at: 2897 2990 2897 Size: 93 5293 BsmBI CGTCTCn'nnnn_ Cuts at: 2498 2498 Size: 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2050 2057 2069 2153 2355 2705 3447 Size: 399 260 7 12 84 202 350 742 Cuts at: 3447 3532 4603 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 260 202 85 84 12 7 BspEI T'CCGG_A Cuts at: 2594 5049 2594 Size: 2455 2931 BspHI T'CATG_A Cuts at: 2214 2233 3275 5005 2214 Size: 19 1042 1730 2595 Fragments arranged by size: 2595 1730 1042 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2741 844 Size: 1289 608 3489 Fragments arranged by size: 3489 1289 608 BsrBI CCG'CTC Cuts at: 1394 1823 2033 3273 1394 Size: 429 210 1240 3507 Fragments arranged by size: 3507 1240 429 210 BsrDI GCAATG_nn' Cuts at: 65 1248 3881 4055 65 Size: 1183 2633 174 1396 Fragments arranged by size: 2633 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4030 4217 1700 Size: 110 2220 187 2869 Fragments arranged by size: 2869 2220 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5386 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5386 BssSI C'ACGA_G Cuts at: 3446 4458 3446 Size: 1012 4374 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5386 BstBI TT'CG_AA Cuts at: 4190 4190 Size: 5386 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5386 BstXI CCAn_nnnn'nTGG Cuts at: 2446 2446 Size: 5386 BstYI r'GATC_y Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381 Size: 744 367 385 17 1452 86 12 346 Cuts at: 5381 1972 Size: 1977 Fragments arranged by size: 1977 1452 744 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3895 Fragments arranged by size: 3895 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4647 Fragments arranged by size: 4647 683 56 BtsI GCAGTG_nn' Cuts at: 2902 3696 3716 2902 Size: 794 20 4572 Fragments arranged by size: 4572 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5386 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5351 DraI TTT'AAA Cuts at: 2690 2690 Size: 5386 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4393 809 Size: 507 3077 1802 Fragments arranged by size: 3077 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2407 2833 3722 Size: 54 1425 50 275 18 577 426 889 Cuts at: 3722 5351 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 577 426 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2833 1537 Size: 275 1021 4090 Fragments arranged by size: 4090 1021 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3316 1353 Size: 1963 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3973 4347 4493 387 Size: 1064 138 2384 374 146 1280 Fragments arranged by size: 2384 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2123 2658 2667 3449 4046 4833 Size: 393 466 25 535 9 782 597 787 Cuts at: 4833 5101 1239 Size: 268 1524 Fragments arranged by size: 1524 787 782 597 535 466 393 268 25 9 EcoO109I rG'GnC_Cy Cuts at: 2046 2047 2122 2046 Size: 1 75 5310 Fragments arranged by size: 5310 75 1 EcoRI G'AATT_C Cuts at: 2426 2695 3073 2426 Size: 269 378 4739 Fragments arranged by size: 4739 378 269 FspI TGC'GCA Cuts at: 3892 3892 Size: 5386 HaeII r_GCGC'y Cuts at: 1678 4533 1678 Size: 2855 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 4027 4059 4101 Size: 32 135 32 450 32 1973 32 42 Cuts at: 4101 4133 1373 Size: 32 2626 Fragments arranged by size: 2626 1973 450 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2730 2976 669 Size: 988 281 792 246 3079 Fragments arranged by size: 3079 988 792 281 246 HindIII A'AGCT_T Cuts at: 748 2670 748 Size: 1922 3464 HpaI GTT'AAC Cuts at: 2976 2976 Size: 5386 KpnI G_GTAC'C Cuts at: 2711 2711 Size: 5386 MfeI C'AATT_G Cuts at: 2985 2985 Size: 5386 MluI A'CGCG_T Cuts at: 5262 5262 Size: 5386 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 4500 4684 1305 Size: 945 2250 184 2007 Fragments arranged by size: 2250 2007 945 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3564 Fragments arranged by size: 3564 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3344 3703 3862 518 Size: 648 2178 359 159 2042 Fragments arranged by size: 2178 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2802 3479 4627 4872 1606 Size: 512 684 677 1148 245 2120 Fragments arranged by size: 2120 1148 684 677 512 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3895 Fragments arranged by size: 3895 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5386 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5386 NotI GC'GGCC_GC Cuts at: 2833 2833 Size: 5386 NspI r_CATG'y Cuts at: 1132 1229 2143 2744 4289 1132 Size: 97 914 601 1545 2229 Fragments arranged by size: 2229 1545 914 601 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5386 PciI A'CATG_T Cuts at: 4285 4285 Size: 5386 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5386 PfoI T'CCnGG_A Cuts at: 2130 2553 2130 Size: 423 4963 PmeI GTTT'AAAC Cuts at: 2690 2690 Size: 5386 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3455 3487 4968 5000 3455 Size: 32 1481 32 3841 Fragments arranged by size: 3841 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 2122 Size: 5386 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5386 PsiI TTA'TAA Cuts at: 2956 2956 Size: 5386 PspOMI G'GGCC_C Cuts at: 2046 2046 Size: 5386 PstI C_TGCA'G Cuts at: 830 2738 830 Size: 1908 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5386 SalI G'TCGA_C Cuts at: 1936 2728 1936 Size: 792 4594 SbfI CC_TGCA'GG Cuts at: 2738 2738 Size: 5386 ScaI AGT'ACT Cuts at: 3634 5346 3634 Size: 1712 3674 SfcI C'TryA_G Cuts at: 826 1045 2734 3869 4550 4741 826 Size: 219 1689 1135 681 191 1471 Fragments arranged by size: 1689 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5386 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5386 SmaI CCC'GGG Cuts at: 1872 2052 2713 1872 Size: 180 661 4545 Fragments arranged by size: 4545 661 180 SmlI C'TyrA_G Cuts at: 820 1017 1943 3490 4391 4653 820 Size: 197 926 1547 901 262 1553 Fragments arranged by size: 1553 1547 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3177 493 Size: 2684 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5386 SphI G_CATG'C Cuts at: 1132 2744 1132 Size: 1612 3774 SspI AAT'ATT Cuts at: 5 52 2561 3310 5 Size: 47 2509 749 2081 Fragments arranged by size: 2509 2081 749 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5386 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 2188 2282 2825 513 Size: 552 939 168 16 94 543 3074 Fragments arranged by size: 3074 939 552 543 168 94 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2267 3413 3430 3583 3768 4213 325 Size: 1255 687 1146 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1146 687 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3632 5344 96 Size: 274 80 33 51 1443 1655 1712 138 Fragments arranged by size: 1712 1655 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5346 5378 5346 Size: 32 5354 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5386 XbaI T'CTAG_A Cuts at: 2722 2722 Size: 5386 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5386 XmaI C'CCGG_G Cuts at: 1870 2050 2711 1870 Size: 180 661 4545 Fragments arranged by size: 4545 661 180 XmnI GAAnn'nnTTC Cuts at: 3515 3515 Size: 5386 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3194 276 Size: 53 83 186 936 1660 2468 Fragments arranged by size: 2468 1660 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI