################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BseRI BsgI BsiWI BsmI BspDI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MaeI MaeIII MamI MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp45I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 721 GACGTC GACGT^C AccIII 764 TCCGGA T^CCGGA AocI 1298 CCTCAGG CC^T[ACGT]AGG Aor51HI 200 AGCGCT AGC^GCT AspI 757 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 384 TGCGCA TGC^GCA BbeI 603 GGCGCC GGCGC^C BbrPI 811 CACGTG CAC^GTG BbvCI 793 GCTGAGG CCTCAGC(-5/-2) BsaI 1453 GAGACC GGTCTC(1/5) BsaWI 764 TCCGGA [AT]^CCGG[AT] BseAI 764 TCCGGA T^CCGGA BsmBI 1029 CGTCTC CGTCTC(1/5) Bsp1407I 1073 TGTACA T^GTACA BspEI 764 TCCGGA T^CCGGA BspHI 617 TCATGA T^CATGA BsrGI 1073 TGTACA T^GTACA Bsu36I 1298 CCTCAGG CC^T[ACGT]AGG DrdI 721 GACGTCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 200 AGCGCT AGC^GCT Eco81I 1298 CCTCAGG CC^T[ACGT]AGG FspI 384 TGCGCA TGC^GCA KasI 603 GGCGCC G^GCGCC MroI 764 TCCGGA T^CCGGA NarI 603 GGCGCC GG^CGCC NcoI 995 CCATGG C^CATGG PflFI 757 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 811 CACGTG CAC^GTG PmlI 811 CACGTG CAC^GTG PshAI 717 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 617 TCATGA T^CATGA SfoI 603 GGCGCC GGC^GCC SspBI 1073 TGTACA T^GTACA Tsp509I 145 AATT ^AATT Tth111I 757 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1055 CCAACCTGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 169 GTCTAC GT^[AC][GT]AC 886 GTCTAC AcyI 603 GGCGCC G[GA]^CG[CT]C 721 GACGTC AfaI 465 GTAC GT^AC 1074 GTAC AflIII 467 ACGTGT A^C[GA][CT]GT 812 ACGTGT AlwNI 856 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 1153 CAGCCCCTG ApaI 27 GGGCCC GGGCC^C 803 GGGCCC BamHI 210 GGATCC G^GATCC 538 GGATCC BglI 595 GCCGGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 688 GCCCTGCGGGC BlpI 327 GCTGAGC GC^T[ACGT]AGC 630 GCTCAGC Bpu1102I 327 GCTGAGC GC^T[ACGT]AGC 630 GCTCAGC BsaAI 466 TACGTG [CT]AC^GT[GA] 811 CACGTG BsaHI 603 GGCGCC G[GA]^CG[CT]C 721 GACGTC BsiEI 278 CGGCCG CG[GA][CT]^CG 359 CGGCCG BspMI 218 ACCTGC ACCTGC(4/8) 890 ACCTGC BsrFI 361 GCCGGC [GA]^CCGG[CT] 818 GCCGGC BsrI 734 ACTGG ACTGG(1/-1) 1360 CCAGT BstXI 824 CCATCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1409 CCATCTGCTTGG CelII 327 GCTGAGC GC^T[ACGT]AGC 630 GCTCAGC Cfr10I 361 GCCGGC [GA]^CCGG[CT] 818 GCCGGC EagI 278 CGGCCG C^GGCCG 359 CGGCCG EclXI 278 CGGCCG C^GGCCG 359 CGGCCG Eco52I 278 CGGCCG C^GGCCG 359 CGGCCG HgaI 417 GACGC GACGC(5/10) 940 GACGC Hin1I 603 GGCGCC G[GA]^CG[CT]C 721 GACGTC NaeI 361 GCCGGC GCC^GGC 818 GCCGGC NgoMIV 361 GCCGGC G^CCGGC 818 GCCGGC PflMI 519 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1244 CCATCACCTGG RsaI 465 GTAC GT^AC 1074 GTAC SapI 1023 GCTCTTC GCTCTTC(1/4) 1209 GCTCTTC StuI 285 AGGCCT AGG^CCT 1093 AGGCCT Van91I 519 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1244 CCATCACCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TspRI TthHB8I XhoII