(Linear) (Six-base) MAPSORT of: KIEE6420.seq Check: 7845 from: 1 to: 1521 KIEE6420.orf With 182 enzymes: SgfI * March 16, 2015 11:31 .. AatII G_ACGT'C Cuts at: 0 725 1521 Size: 725 796 AccI GT'mk_AC Cuts at: 0 170 887 1521 Size: 170 717 634 Fragments arranged by size: 717 634 170 AfeI AGC'GCT Cuts at: 0 202 1521 Size: 202 1319 AflIII A'CryG_T Cuts at: 0 467 812 1521 Size: 467 345 709 Fragments arranged by size: 709 467 345 AleI CACnn'nnGTG Cuts at: 0 1186 1521 Size: 1186 335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1036 1521 Size: 1036 485 AlwNI CAG_nnn'CTG Cuts at: 0 861 1158 1521 Size: 861 297 363 Fragments arranged by size: 861 363 297 ApaI G_GGCC'C Cuts at: 0 31 807 1521 Size: 31 776 714 Fragments arranged by size: 776 714 31 AvaI C'yCGr_G Cuts at: 0 49 668 1450 1521 Size: 49 619 782 71 Fragments arranged by size: 782 619 71 49 BamHI G'GATC_C Cuts at: 0 210 538 1521 Size: 210 328 983 Fragments arranged by size: 983 328 210 BanI G'GyrC_C Cuts at: 0 603 1188 1386 1521 Size: 603 585 198 135 Fragments arranged by size: 603 585 198 135 BanII G_rGCy'C Cuts at: 0 31 443 504 807 1105 1307 1521 Size: 31 412 61 303 298 202 214 Fragments arranged by size: 412 303 298 214 202 61 31 BbeI G_GCGC'C Cuts at: 0 607 1521 Size: 607 914 BbvCI CC'TCA_GC Cuts at: 0 794 1521 Size: 794 727 BglI GCCn_nnn'nGGC Cuts at: 0 601 694 1521 Size: 601 93 827 Fragments arranged by size: 827 601 93 BlpI GC'TnA_GC Cuts at: 0 328 631 1521 Size: 328 303 890 Fragments arranged by size: 890 328 303 Bme1580I G_kGCm'C Cuts at: 0 31 807 1193 1287 1451 1521 Size: 31 776 386 94 164 70 Fragments arranged by size: 776 386 164 94 70 31 BmrI ACTGGGnnnn_n' Cuts at: 0 744 1521 Size: 744 777 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 1366 1492 1521 Size: 217 1149 126 29 Fragments arranged by size: 1149 217 126 29 Bpu10I CC'TnA_GC Cuts at: 0 794 1521 Size: 794 727 BsaI GGTCTCn'nnnn_ Cuts at: 0 1447 1521 Size: 1447 74 BsaAI yAC'GTr Cuts at: 0 468 813 1521 Size: 468 345 708 Fragments arranged by size: 708 468 345 BsaHI Gr'CG_yC Cuts at: 0 604 722 1521 Size: 604 118 799 Fragments arranged by size: 799 604 118 BsaWI w'CCGG_w Cuts at: 0 764 1521 Size: 764 757 BseYI C'CCAG_C Cuts at: 0 15 189 518 1235 1521 Size: 15 174 329 717 286 Fragments arranged by size: 717 329 286 174 15 BsiEI CG_ry'CG Cuts at: 0 281 362 1521 Size: 281 81 1159 Fragments arranged by size: 1159 281 81 BsiHKAI G_wGCw'C Cuts at: 0 140 614 1372 1521 Size: 140 474 758 149 Fragments arranged by size: 758 474 149 140 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1035 1521 Size: 1035 486 Bsp1286I G_dGCh'C Cuts at: 0 31 140 443 504 614 807 1105 1193 Size: 31 109 303 61 110 193 298 88 Cuts at: 1193 1287 1307 1372 1451 1521 Size: 94 20 65 79 70 Fragments arranged by size: 303 298 193 110 109 94 88 79 70 65 61 31 20 BspEI T'CCGG_A Cuts at: 0 764 1521 Size: 764 757 BspHI T'CATG_A Cuts at: 0 617 1521 Size: 617 904 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 227 899 1521 Size: 227 672 622 Fragments arranged by size: 672 622 227 BsrFI r'CCGG_y Cuts at: 0 361 818 1521 Size: 361 457 703 Fragments arranged by size: 703 457 361 BsrGI T'GTAC_A Cuts at: 0 1073 1521 Size: 1073 448 BstXI CCAn_nnnn'nTGG Cuts at: 0 831 1416 1521 Size: 831 585 105 Fragments arranged by size: 831 585 105 BstYI r'GATC_y Cuts at: 0 210 538 749 1521 Size: 210 328 211 772 Fragments arranged by size: 772 328 211 210 Bsu36I CC'TnA_GG Cuts at: 0 1299 1521 Size: 1299 222 BtgI C'CryG_G Cuts at: 0 574 830 995 1229 1287 1521 Size: 574 256 165 234 58 234 Fragments arranged by size: 574 256 234 234 165 58 BtsI GCAGTG_nn' Cuts at: 0 140 536 1112 1521 Size: 140 396 576 409 Fragments arranged by size: 576 409 396 140 DrdI GACnn_nn'nnGTC Cuts at: 0 727 1521 Size: 727 794 EaeI y'GGCC_r Cuts at: 0 119 278 359 593 1037 1199 1316 1406 Size: 119 159 81 234 444 162 117 90 Cuts at: 1406 1521 Size: 115 Fragments arranged by size: 444 234 162 159 119 117 115 90 81 EagI C'GGCC_G Cuts at: 0 278 359 1521 Size: 278 81 1162 Fragments arranged by size: 1162 278 81 EarI CTCTTCn'nnn_ Cuts at: 0 677 1030 1216 1521 Size: 677 353 186 305 Fragments arranged by size: 677 353 305 186 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 1366 1492 1521 Size: 217 1149 126 29 Fragments arranged by size: 1149 217 126 29 EcoO109I rG'GnC_Cy Cuts at: 0 27 92 687 804 1240 1269 1508 1521 Size: 27 65 595 117 436 29 239 13 Fragments arranged by size: 595 436 239 117 65 29 27 13 FspI TGC'GCA Cuts at: 0 386 1521 Size: 386 1135 FspAI rTGC'GCAy Cuts at: 0 386 1521 Size: 386 1135 HaeII r_GCGC'y Cuts at: 0 204 582 607 700 1521 Size: 204 378 25 93 821 Fragments arranged by size: 821 378 204 93 25 KasI G'GCGC_C Cuts at: 0 603 1521 Size: 603 918 MscI TGG'CCA Cuts at: 0 121 1201 1318 1521 Size: 121 1080 117 203 Fragments arranged by size: 1080 203 121 117 MslI CAynn'nnrTG Cuts at: 0 460 529 829 1186 1521 Size: 460 69 300 357 335 Fragments arranged by size: 460 357 335 300 69 MspA1I CmG'CkG Cuts at: 0 12 277 346 448 861 916 1036 1171 Size: 12 265 69 102 413 55 120 135 Cuts at: 1171 1322 1521 Size: 151 199 Fragments arranged by size: 413 265 199 151 135 120 102 69 55 12 NaeI GCC'GGC Cuts at: 0 363 820 1521 Size: 363 457 701 Fragments arranged by size: 701 457 363 NarI GG'CG_CC Cuts at: 0 604 1521 Size: 604 917 NcoI C'CATG_G Cuts at: 0 995 1521 Size: 995 526 NgoMIV G'CCGG_C Cuts at: 0 361 818 1521 Size: 361 457 703 Fragments arranged by size: 703 457 361 PasI CC'CwG_GG Cuts at: 0 267 1521 Size: 267 1254 PflMI CCAn_nnn'nTGG Cuts at: 0 525 1250 1521 Size: 525 725 271 Fragments arranged by size: 725 525 271 PfoI T'CCnGG_A Cuts at: 0 752 1521 Size: 752 769 PmlI CAC'GTG Cuts at: 0 813 1521 Size: 813 708 PpuMI rG'GwC_Cy Cuts at: 0 92 1240 1269 1508 1521 Size: 92 1148 29 239 13 Fragments arranged by size: 1148 239 92 29 13 PshAI GACnn'nnGTC Cuts at: 0 721 1521 Size: 721 800 PspOMI G'GGCC_C Cuts at: 0 27 803 1521 Size: 27 776 718 Fragments arranged by size: 776 718 27 PstI C_TGCA'G Cuts at: 0 101 857 896 1521 Size: 101 756 39 625 Fragments arranged by size: 756 625 101 39 PvuII CAG'CTG Cuts at: 0 346 448 861 1322 1521 Size: 346 102 413 461 199 Fragments arranged by size: 461 413 346 199 102 SanDI GG'GwC_CC Cuts at: 0 92 1240 1521 Size: 92 1148 281 Fragments arranged by size: 1148 281 92 SapI GCTCTTCn'nnn_ Cuts at: 0 1030 1216 1521 Size: 1030 186 305 Fragments arranged by size: 1030 305 186 SfcI C'TryA_G Cuts at: 0 97 853 892 1521 Size: 97 756 39 629 Fragments arranged by size: 756 629 97 39 SfoI GGC'GCC Cuts at: 0 605 1521 Size: 605 916 StuI AGG'CCT Cuts at: 0 287 1095 1521 Size: 287 808 426 Fragments arranged by size: 808 426 287 StyI C'CwwG_G Cuts at: 0 206 558 932 995 1457 1521 Size: 206 352 374 63 462 64 Fragments arranged by size: 462 374 352 206 64 63 TatI w'GTAC_w Cuts at: 0 1073 1521 Size: 1073 448 Tth111I GACn'n_nGTC Cuts at: 0 760 1521 Size: 760 761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1062 1521 Size: 1062 459 ZraI GAC'GTC Cuts at: 0 723 1521 Size: 723 798 Enzymes that do cut: AatII AccI AfeI AflIII AleI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BsrGI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EarI Eco57MI EcoO109I FspI FspAI HaeII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SanDI SapI SfcI SfoI StuI StyI TatI Tth111I XcmI ZraI Enzymes that do not cut: AarI Acc65I AclI AcuI AflII AgeI AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI XbaI XhoI XmaI XmnI