################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI NarI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcsI 284 AAATTC [GA]^AATT[CT] AfaI 801 GTAC GT^AC Alw44I 114 GTGCAC G^TGCAC AlwNI 88 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 114 GTGCAC G^TGCAC ApoI 284 AAATTC [GA]^AATT[CT] AvaII 187 GGACC G^G[AT]CC BanI 102 GGCACC G^G[CT][GA]CC BcgI 353 CGAAATTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 660 GGATAC GTATCC(6/5) BsgI 220 CTGCAC GTGCAG(16/14) BsmAI 233 GAGAC GTCTC(1/5) BsmFI 343 GGGAC GGGAC(10/14) BspMI 148 GCAGGT ACCTGC(4/8) BsrBI 157 GAGCGG CCGCTC(-3/-3) BssHII 144 GCGCGC G^CGCGC BstAPI 87 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 497 CCAATTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 539 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 163 CGGCCG [CT]^GGCC[GA] EagI 163 CGGCCG C^GGCCG EclXI 163 CGGCCG C^GGCCG Eco52I 163 CGGCCG C^GGCCG EcoO109I 539 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 689 CCTTGG C^C[AT][AT]GG FauI 70 CCCGC CCCGC(4/6) HindIII 239 AAGCTT A^AGCTT HphI 433 GGTGA GGTGA(8/7) PflMI 711 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 646 GAGTC GAGTC(4/5) PstI 94 CTGCAG CTGCA^G RsaI 801 GTAC GT^AC SacI 772 GAGCTC GAGCT^C SfaNI 413 GCATC GCATC(5/9) SfcI 94 CTGCAG C^T[GA][CT]AG SmlI 823 CTCAAG C^T[CT][GA]AG StyI 689 CCTTGG C^C[AT][AT]GG TfiI 685 GATTC G^A[AT]TC TspRI 639 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 711 CCACCTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BanII 36 GGGCTC G[GA]GC[CT]^C 772 GAGCTC BbsI 586 GAAGAC GAAGAC(2/6) 817 GAAGAC BglI 78 GCCTTTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 123 GCCCTTCGGGC BlpI 135 GCTCAGC GC^T[ACGT]AGC 774 GCTCAGC BpmI 317 CTGGAG CTGGAG(16/14) 787 CTGGAG Bpu1102I 135 GCTCAGC GC^T[ACGT]AGC 774 GCTCAGC BpuAI 586 GAAGAC GAAGAC(2/6) 817 GAAGAC BseRI 39 CTCCTC GAGGAG(10/8) 436 GAGGAG BsiEI 163 CGGCCG CG[GA][CT]^CG 732 CGACCG BsrFI 49 GCCGGC [GA]^CCGG[CT] 99 GCCGGC BsrI 640 ACTGG ACTGG(1/-1) 695 ACTGG BstF5I 414 CATCC GGATG(2/0) 570 GGATG BstNI 786 CCTGG CC^[AT]GG 795 CCAGG CelII 135 GCTCAGC GC^T[ACGT]AGC 774 GCTCAGC Cfr10I 49 GCCGGC [GA]^CCGG[CT] 99 GCCGGC EcoRII 786 CCTGG ^CC[AT]GG 795 CCAGG FokI 414 CATCC GGATG(9/13) 570 GGATG HinfI 646 GAGTC G^A[ACGT]TC 685 GATTC MaeIII 391 GTCAC ^GT[ACGT]AC 602 GTGAC MseI 269 TTAA T^TAA 404 TTAA MvaI 786 CCTGG CC^[AT]GG 795 CCAGG NaeI 49 GCCGGC GCC^GGC 99 GCCGGC NgoMIV 49 GCCGGC G^CCGGC 99 GCCGGC Tru9I 269 TTAA T^TAA 404 TTAA Tsp45I 391 GTCAC ^GT[GC]AC 602 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AluI AspHI BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstUI Cac8I CfoI Cfr13I DdeI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI Tsp509I