(Circular) (Six-base) MAPSORT of: pFN21AE1430.seq Check: 4116 from: 1 to: 5551 pFN21AE1430 5551 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2894 1937 Size: 957 4594 Acc65I G'GTAC_C Cuts at: 2872 2872 Size: 5551 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3614 4998 5266 1632 Size: 1982 1384 268 1917 Fragments arranged by size: 1982 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 4450 5427 1203 Size: 3247 977 1327 Fragments arranged by size: 3247 1327 977 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 2098 3294 4866 2098 Size: 1196 1572 2783 Fragments arranged by size: 2783 1572 1196 ApaLI G'TGCA_C Cuts at: 2119 3608 4764 2119 Size: 1489 1156 2906 Fragments arranged by size: 2906 1489 1156 ApoI r'AATT_y Cuts at: 1493 2289 2860 3086 3238 5347 1493 Size: 796 571 226 152 2109 1697 Fragments arranged by size: 2109 1697 796 571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2876 1870 Size: 73 933 4545 Fragments arranged by size: 4545 933 73 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2107 2872 4327 618 Size: 325 217 627 320 765 1455 1842 Fragments arranged by size: 1842 1455 765 627 325 320 217 BanII G_rGCy'C Cuts at: 1391 2045 2781 2870 1391 Size: 654 736 89 4072 Fragments arranged by size: 4072 736 654 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2598 2829 928 Size: 971 699 231 3650 Fragments arranged by size: 3650 971 699 231 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2347 2381 3742 3776 1903 Size: 34 50 34 326 34 1361 34 3678 Fragments arranged by size: 3678 1361 326 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2659 3443 4653 2659 Size: 784 1210 3557 Fragments arranged by size: 3557 1210 784 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2089 2134 4162 Size: 107 122 71 631 605 417 45 2028 Cuts at: 4162 136 Size: 1525 Fragments arranged by size: 2028 1525 631 605 417 122 107 71 45 BglII A'GATC_T Cuts at: 5546 5546 Size: 5551 BlpI GC'TnA_GC Cuts at: 2141 2780 2968 2141 Size: 639 188 4724 Fragments arranged by size: 4724 639 188 Bme1580I G_kGCm'C Cuts at: 1790 2031 2123 3612 4768 1790 Size: 241 92 1489 1156 2573 Fragments arranged by size: 2573 1489 1156 241 92 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3282 4240 Size: 416 53 83 399 24 1293 572 958 Cuts at: 4240 442 Size: 1753 Fragments arranged by size: 1753 1293 958 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 2813 4211 1239 Size: 1104 470 1398 2579 Fragments arranged by size: 2579 1398 1104 470 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2813 3676 4541 4839 2813 Size: 863 865 298 3525 Fragments arranged by size: 3525 865 863 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4214 882 Size: 617 2715 2219 Fragments arranged by size: 2715 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3342 493 Size: 711 2138 2702 Fragments arranged by size: 2702 2138 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3358 3740 4375 275 Size: 53 83 186 936 1825 382 635 1451 Fragments arranged by size: 1825 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3984 4382 4656 4803 5214 3984 Size: 398 274 147 411 4321 Fragments arranged by size: 4321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2482 2512 1178 Size: 30 165 30 1079 30 4217 Fragments arranged by size: 4217 1079 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2456 1186 Size: 849 421 4281 Fragments arranged by size: 4281 849 421 BseYI C'CCAG_C Cuts at: 2038 4754 5250 2038 Size: 2716 496 2339 Fragments arranged by size: 2716 2339 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2210 1163 Size: 573 474 4504 Fragments arranged by size: 4504 573 474 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2171 2740 3001 3762 4790 1540 Size: 275 186 170 569 261 761 1028 2301 Fragments arranged by size: 2301 1028 761 569 275 261 186 170 BsiHKAI G_wGCw'C Cuts at: 2123 2781 2799 3612 3697 4768 2123 Size: 658 18 813 85 1071 2906 Fragments arranged by size: 2906 1071 813 658 85 18 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2045 2123 2781 2799 2870 3612 Size: 399 241 14 78 658 18 71 742 Cuts at: 3612 3697 4768 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 658 399 241 85 78 71 18 14 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2144 2906 844 Size: 1300 762 3489 Fragments arranged by size: 3489 1300 762 BsrBI CCG'CTC Cuts at: 1394 1823 2164 3438 1394 Size: 429 341 1274 3507 Fragments arranged by size: 3507 1274 429 341 BsrDI GCAATG_nn' Cuts at: 65 1248 4046 4220 65 Size: 1183 2798 174 1396 Fragments arranged by size: 2798 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2054 2104 4195 4382 1700 Size: 110 244 50 2091 187 2869 Fragments arranged by size: 2869 2091 244 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 2149 1925 Size: 224 5327 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 794 Size: 1304 4247 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5551 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2881 3248 3633 3650 5102 5188 5200 5546 Size: 909 367 385 17 1452 86 12 346 Cuts at: 5546 1972 Size: 1977 Fragments arranged by size: 1977 1452 909 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4999 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 809 Size: 507 3242 1802 Fragments arranged by size: 3242 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2168 2998 3887 Size: 54 1425 50 275 18 338 830 889 Cuts at: 3887 5516 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 830 338 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2168 2998 1537 Size: 275 356 830 4090 Fragments arranged by size: 4090 830 356 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3481 1353 Size: 2128 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2779 2779 Size: 5551 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 2813 3614 4211 4998 5266 1239 Size: 393 711 470 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 711 597 470 393 268 EcoO109I rG'GnC_Cy Cuts at: 2545 2545 Size: 5551 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 4698 1678 Size: 3020 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2482 2514 2600 2632 4192 Size: 32 135 32 910 32 86 32 1560 Cuts at: 4192 4224 4266 4298 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1560 910 135 86 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 3141 669 Size: 988 281 957 246 3079 Fragments arranged by size: 3079 988 957 281 246 HindIII A'AGCT_T Cuts at: 748 2244 748 Size: 1496 4055 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2876 2876 Size: 5551 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2535 2563 4665 4849 1305 Size: 1230 28 2102 184 2007 Fragments arranged by size: 2102 2007 1230 184 28 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3729 Fragments arranged by size: 3729 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3509 3868 4027 518 Size: 648 2343 359 159 2042 Fragments arranged by size: 2343 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2967 3644 4792 5037 1606 Size: 1361 677 1148 245 2120 Fragments arranged by size: 2120 1361 1148 677 245 NaeI GCC'GGC Cuts at: 2056 2106 2056 Size: 50 5501 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4999 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NgoMIV G'CCGG_C Cuts at: 2054 2104 2054 Size: 50 5501 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2909 4454 1132 Size: 97 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5551 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2722 1108 Size: 1614 3937 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 2522 3121 2522 Size: 599 4952 PstI C_TGCA'G Cuts at: 830 2103 2903 830 Size: 1273 800 3478 Fragments arranged by size: 3478 1273 800 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 SacI G_AGCT'C Cuts at: 2781 2781 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2099 2899 4034 4715 4906 826 Size: 219 1054 800 1135 681 191 1471 Fragments arranged by size: 1471 1135 1054 800 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5551 SmaI CCC'GGG Cuts at: 1872 2878 1872 Size: 1006 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 3655 4556 4818 820 Size: 197 926 885 827 901 262 1553 Fragments arranged by size: 1553 926 901 885 827 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5551 StyI C'CwwG_G Cuts at: 513 1065 2694 2990 513 Size: 552 1629 296 3074 Fragments arranged by size: 3074 1629 552 296 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2754 3578 3595 3748 3933 4378 325 Size: 1255 1174 824 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1174 824 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2805 3797 5509 Size: 274 80 33 51 1443 828 992 1712 Cuts at: 5509 96 Size: 138 Fragments arranged by size: 1712 1443 992 828 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5511 5543 5511 Size: 32 5519 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2876 1870 Size: 1006 4545 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI