(Circular) (Six-base) MAPSORT of: pFN21AB5957.seq Check: 6622 from: 1 to: 6244 pFN21AB5957 6244 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2057 2057 Size: 6244 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2485 4054 278 Size: 53 83 186 936 949 1569 2468 Fragments arranged by size: 2468 1569 949 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2333 3409 4307 5691 5959 1632 Size: 701 1076 898 1384 268 1917 Fragments arranged by size: 1917 1384 1076 898 701 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 2154 5143 6120 1203 Size: 951 2989 977 1327 Fragments arranged by size: 2989 1327 977 951 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 2871 4973 2871 Size: 2102 4142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 3043 3987 5559 3043 Size: 944 1572 3728 Fragments arranged by size: 3728 1572 944 ApaI G_GGCC'C Cuts at: 2271 2491 2271 Size: 220 6024 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 3553 3779 3931 6040 1493 Size: 2060 226 152 2109 1697 Fragments arranged by size: 2109 2060 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2399 3056 3439 3569 1870 Size: 73 456 657 383 130 4545 Fragments arranged by size: 4545 657 456 383 130 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 2654 3565 5020 618 Size: 325 217 627 424 443 911 1455 1842 Fragments arranged by size: 1842 1455 911 627 443 424 325 217 BanII G_rGCy'C Cuts at: 1391 2082 2271 2310 2491 2745 3092 3262 3563 Size: 691 189 39 181 254 347 170 301 Cuts at: 3563 1391 Size: 4072 Fragments arranged by size: 4072 691 347 301 254 189 181 170 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3177 928 Size: 971 1278 3995 Fragments arranged by size: 3995 1278 971 BbvCI CC'TCA_GC Cuts at: 2690 3202 2690 Size: 512 5732 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2579 2613 2683 2717 4435 4469 1903 Size: 34 642 34 70 34 1718 34 3678 Fragments arranged by size: 3678 1718 642 70 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 3465 1548 Size: 1917 4327 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2209 2266 3415 Size: 107 122 71 631 605 537 57 1149 Cuts at: 3415 4855 136 Size: 1440 1525 Fragments arranged by size: 1525 1440 1149 631 605 537 122 107 71 57 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 2706 3661 2706 Size: 955 5289 Bme1580I G_kGCm'C Cuts at: 1790 2141 2165 2271 2491 4305 5461 1790 Size: 351 24 106 220 1814 1156 2573 Fragments arranged by size: 2573 1814 1156 351 220 106 24 BmgBI CAC'GTC Cuts at: 1318 2956 1318 Size: 1638 4606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2659 3975 4933 Size: 416 53 83 399 24 1242 1316 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1753 1316 1242 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2531 2531 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2799 3287 4904 1239 Size: 1560 488 1617 2579 Fragments arranged by size: 2579 1617 1560 488 Bpu10I CC'TnA_GC Cuts at: 2690 3202 2690 Size: 512 5732 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2539 2636 4369 5234 5532 2539 Size: 97 1733 865 298 3251 Fragments arranged by size: 3251 1733 865 298 97 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 2171 3349 3527 4907 882 Size: 617 537 135 1178 178 1380 2219 Fragments arranged by size: 2219 1380 1178 617 537 178 135 BsaAI yAC'GTr Cuts at: 493 1204 4035 493 Size: 711 2831 2702 Fragments arranged by size: 2831 2702 711 BsaBI GATnn'nnATC Cuts at: 2342 6238 2342 Size: 3896 2348 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2482 4051 4433 5068 Size: 53 83 186 936 949 1569 382 635 Cuts at: 5068 275 Size: 1451 Fragments arranged by size: 1569 1451 949 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4677 5075 5349 5496 5907 4677 Size: 398 274 147 411 5014 Fragments arranged by size: 5014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2579 2609 1178 Size: 30 165 30 1176 30 4813 Fragments arranged by size: 4813 1176 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2417 2633 3450 1186 Size: 1156 75 216 817 3980 Fragments arranged by size: 3980 1156 817 216 75 BseYI C'CCAG_C Cuts at: 2133 2984 5447 5943 2133 Size: 851 2463 496 2434 Fragments arranged by size: 2463 2434 851 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3202 3474 1163 Size: 573 1466 272 3933 Fragments arranged by size: 3933 1466 573 272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2261 2362 3694 4455 5483 1540 Size: 275 186 260 101 1332 761 1028 2301 Fragments arranged by size: 2301 1332 1028 761 275 260 186 101 BsiHKAI G_wGCw'C Cuts at: 2376 2745 4305 4390 5461 2376 Size: 369 1560 85 1071 3159 Fragments arranged by size: 3159 1560 1071 369 85 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 BsmBI CGTCTCn'nnnn_ Cuts at: 2473 2473 Size: 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2141 2165 2271 2310 2376 2491 Size: 399 292 59 24 106 39 66 115 Cuts at: 2491 2745 3092 3262 3563 4305 4390 5461 1391 Size: 254 347 170 301 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 347 301 292 254 170 115 106 85 66 59 39 24 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 4133 5863 4133 Size: 1730 4514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 3051 3599 844 Size: 1213 994 548 3489 Fragments arranged by size: 3489 1213 994 548 BsrBI CCG'CTC Cuts at: 1394 1823 2257 2364 2398 3301 4131 1394 Size: 429 434 107 34 903 830 3507 Fragments arranged by size: 3507 903 830 434 429 107 34 BsrDI GCAATG_nn' Cuts at: 65 1248 4739 4913 65 Size: 1183 3491 174 1396 Fragments arranged by size: 3491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 4888 5075 1700 Size: 110 398 2680 187 2869 Fragments arranged by size: 2869 2680 398 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6244 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6244 BssSI C'ACGA_G Cuts at: 2505 4304 5316 2505 Size: 1799 1012 3433 Fragments arranged by size: 3433 1799 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6244 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstXI CCAn_nnnn'nTGG Cuts at: 2350 2350 Size: 6244 BstYI r'GATC_y Cuts at: 1972 3574 3941 4326 4343 5795 5881 5893 6239 Size: 1602 367 385 17 1452 86 12 346 Cuts at: 6239 1972 Size: 1977 Fragments arranged by size: 1977 1602 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2377 3528 513 Size: 552 939 373 1151 3229 Fragments arranged by size: 3229 1151 939 552 373 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3088 487 Size: 683 56 1862 3643 Fragments arranged by size: 3643 1862 683 56 BtsI GCAGTG_nn' Cuts at: 2174 3234 3760 4554 4574 2174 Size: 1060 526 794 20 3844 Fragments arranged by size: 3844 1060 794 526 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 2454 2472 3548 2454 Size: 18 1076 5150 Fragments arranged by size: 5150 1076 18 DraIII CAC_nnn'GTG Cuts at: 2389 2389 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2155 5251 809 Size: 507 839 3096 1802 Fragments arranged by size: 3096 1802 839 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2098 2217 Size: 54 1425 50 275 18 192 76 119 Cuts at: 2217 2258 2380 2631 3502 3691 4580 6209 8 Size: 41 122 251 871 189 889 1629 43 Fragments arranged by size: 1629 1425 889 871 275 251 192 189 122 119 76 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2258 3691 1537 Size: 275 446 1433 4090 Fragments arranged by size: 4090 1433 446 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2471 2709 4174 1353 Size: 1118 238 1465 3423 Fragments arranged by size: 3423 1465 1118 238 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4831 5205 5351 387 Size: 1064 138 3242 374 146 1280 Fragments arranged by size: 3242 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2743 2743 Size: 6244 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2333 2799 3287 3409 4307 4904 5691 Size: 393 701 466 488 122 898 597 787 Cuts at: 5691 5959 1239 Size: 268 1524 Fragments arranged by size: 1524 898 787 701 597 488 466 393 268 122 EcoNI CCTnn'n_nnAGG Cuts at: 2036 3035 2036 Size: 999 5245 EcoO109I rG'GnC_Cy Cuts at: 2267 3040 2267 Size: 773 5471 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 FspI TGC'GCA Cuts at: 2249 4750 2249 Size: 2501 3743 HaeII r_GCGC'y Cuts at: 1678 5391 1678 Size: 3713 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2246 2278 2797 2829 2857 Size: 32 135 32 674 32 519 32 28 Cuts at: 2857 2889 3052 3084 4885 4917 4959 4991 1373 Size: 32 163 32 1801 32 42 32 2626 Fragments arranged by size: 2626 1801 674 519 163 135 42 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3058 5358 5542 1305 Size: 1753 2300 184 2007 Fragments arranged by size: 2300 2007 1753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 2633 3504 10 Size: 54 1425 343 387 414 871 2750 Fragments arranged by size: 2750 1425 871 414 387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2348 4202 4561 4720 518 Size: 648 1182 1854 359 159 2042 Fragments arranged by size: 2042 1854 1182 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2130 2245 2379 2569 3307 3660 4337 5485 Size: 524 115 134 190 738 353 677 1148 Cuts at: 5485 5730 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 738 677 524 353 245 190 134 115 NcoI C'CATG_G Cuts at: 513 1065 2004 3528 513 Size: 552 939 1524 3229 Fragments arranged by size: 3229 1524 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 3691 3691 Size: 6244 NspI r_CATG'y Cuts at: 1132 1229 2200 3602 5147 1132 Size: 97 971 1402 1545 2229 Fragments arranged by size: 2229 1545 1402 971 97 PasI CC'CwG_GG Cuts at: 1348 2491 1348 Size: 1143 5101 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 3310 1108 Size: 2202 4042 PfoI T'CCnGG_A Cuts at: 2012 2012 Size: 6244 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3040 3040 Size: 6244 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspOMI G'GGCC_C Cuts at: 2267 2487 2267 Size: 220 6024 PspXI vC'TCGA_Gb Cuts at: 2399 2399 Size: 6244 PstI C_TGCA'G Cuts at: 830 2037 2067 2751 3596 830 Size: 1207 30 684 845 3478 Fragments arranged by size: 3478 1207 845 684 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2245 2569 2245 Size: 324 5920 SacI G_AGCT'C Cuts at: 2745 2745 Size: 6244 SacII CC_GC'GG Cuts at: 2380 2380 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 2751 3596 2751 Size: 845 5399 ScaI AGT'ACT Cuts at: 2069 4492 6204 2069 Size: 2423 1712 2109 Fragments arranged by size: 2423 2109 1712 SfcI C'TryA_G Cuts at: 826 1045 2033 2063 2747 3592 4727 5408 5599 Size: 219 988 30 684 845 1135 681 191 Cuts at: 5599 826 Size: 1471 Fragments arranged by size: 1471 1135 988 845 684 681 219 191 30 SfiI GGCCn_nnn'nGGCC Cuts at: 2266 3415 2266 Size: 1149 5095 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 3571 1872 Size: 1699 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 2518 2615 4348 5249 5511 Size: 197 926 456 119 97 1733 901 262 Cuts at: 5511 820 Size: 1553 Fragments arranged by size: 1733 1553 926 901 456 262 197 119 97 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 3254 4168 5 Size: 47 3202 914 2081 Fragments arranged by size: 3202 2081 914 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6244 StyI C'CwwG_G Cuts at: 513 1065 2004 2801 3528 3683 513 Size: 552 939 797 727 155 3074 Fragments arranged by size: 3074 939 797 727 552 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2678 4271 4288 4441 4626 5071 325 Size: 1255 1098 1593 17 153 185 445 1498 Fragments arranged by size: 1593 1498 1255 1098 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2319 4490 Size: 274 80 33 51 1443 90 252 2171 Cuts at: 4490 6202 96 Size: 1712 138 Fragments arranged by size: 2171 1712 1443 274 252 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3145 3177 6204 6236 3145 Size: 32 3027 32 3153 Fragments arranged by size: 3153 3027 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6244 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2212 2212 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 2399 1943 Size: 456 5788 XmaI C'CCGG_G Cuts at: 1870 3569 1870 Size: 1699 4545 XmnI GAAnn'nnTTC Cuts at: 3218 4373 3218 Size: 1155 5089 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2483 4052 276 Size: 53 83 186 936 949 1569 2468 Fragments arranged by size: 2468 1569 949 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI