Restriction map for
pFN21AB5844
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I AlwNI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbrPI BbsI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsiWI
BsmBI BsmI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KpnI KspI MamI
MfeI MluI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1129 TCCGGA T^CCGGA
AflIII 440 ACGTGT A^C[GA][CT]GT
ApaI 1016 GGGCCC GGGCC^C
Asp700I 456 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 256 GAGCAC G[AT]GC[AT]^C
BalI 1211 TGGCCA TGG^CCA
BbvCI 985 CCTCAGC CCTCAGC(-5/-2)
BcgI 869 GCATCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 439 TACGTG [CT]AC^GT[GA]
BsaWI 1129 TCCGGA [AT]^CCGG[AT]
BseAI 1129 TCCGGA T^CCGGA
BsiEI 1011 CGACCG CG[GA][CT]^CG
BsiHKAI 256 GAGCAC G[AT]GC[AT]^C
Bsp1407I 218 TGTACA T^GTACA
BspDI 739 ATCGAT AT^CGAT
BspEI 1129 TCCGGA T^CCGGA
BspMI 749 ACCTGC ACCTGC(4/8)
BsrBI 151 CCGCTC CCGCTC(-3/-3)
BsrFI 836 GCCGGC [GA]^CCGG[CT]
BsrGI 218 TGTACA T^GTACA
BstEII 159 GGTTACC G^GT[ACGT]ACC
BstPI 159 GGTTACC G^GT[ACGT]ACC
BstYI 920 AGATCC [GA]^GATC[CT]
Cfr10I 836 GCCGGC [GA]^CCGG[CT]
ClaI 739 ATCGAT AT^CGAT
DrdI 301 GACAACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 159 GGTTACC G^GT[ACGT]ACC
EcoT14I 200 CCTTGG C^C[AT][AT]GG
MflI 920 AGATCC [GA]^GATC[CT]
MluNI 1211 TGGCCA TGG^CCA
MroI 1129 TCCGGA T^CCGGA
MscI 1211 TGGCCA TGG^CCA
MseI 418 TTAA T^TAA
NaeI 836 GCCGGC GCC^GGC
NgoMIV 836 GCCGGC G^CCGGC
PaeR7I 946 CTCGAG C^TCGAG
PpuMI 542 AGGACCC [GA]G^G[AT]CC[CT]
PstI 465 CTGCAG CTGCA^G
PvuII 1386 CAGCTG CAG^CTG
SapI 616 GAAGAGC GCTCTTC(1/4)
ScaI 176 AGTACT AGT^ACT
SfcI 465 CTGCAG C^T[GA][CT]AG
SmaI 93 CCCGGG CCC^GGG
SspBI 218 TGTACA T^GTACA
StyI 200 CCTTGG C^C[AT][AT]GG
TfiI 1080 GATTC G^A[AT]TC
Tru9I 418 TTAA T^TAA
XhoI 946 CTCGAG C^TCGAG
XhoII 920 AGATCC [GA]^GATC[CT]
XmaCI 93 CCCGGG C^CCGGG
XmaI 93 CCCGGG C^CCGGG
XmnI 456 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 22 GGCGCC G[GA]^CG[CT]C
1243 GGCGCC
AhdI 360 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
475 GACCATGTGTC
AlwI 742 GATCC GGATC(4/5)
921 GATCC
AocI 933 CCTGAGG CC^T[ACGT]AGG
1152 CCTAAGG
AspEI 360 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
475 GACCATGTGTC
BanI 22 GGCGCC G^G[CT][GA]CC
1243 GGCGCC
BbeI 22 GGCGCC GGCGC^C
1243 GGCGCC
BfaI 17 CTAG C^TAG
338 CTAG
BglI 64 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1151 GCCTAAGGGGC
BsaHI 22 GGCGCC G[GA]^CG[CT]C
1243 GGCGCC
BseRI 936 GAGGAG GAGGAG(10/8)
1258 GAGGAG
BsmFI 96 GGGAC GGGAC(10/14)
473 GGGAC
BstAPI 525 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
555 GCAGCCCGTGC
Bsu36I 933 CCTGAGG CC^T[ACGT]AGG
1152 CCTAAGG
DraII 542 AGGACCC [GA]G^G[ACGT]CC[CT]
1016 GGGCCCC
DsaI 1055 CCACGG C^C[GA][CT]GG
1229 CCACGG
EaeI 1058 CGGCCA [CT]^GGCC[GA]
1211 TGGCCA
Eam1105I 360 GACCTTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
475 GACCATGTGTC
Eco81I 933 CCTGAGG CC^T[ACGT]AGG
1152 CCTAAGG
EcoO109I 542 AGGACCC [GA]G^G[ACGT]CC[CT]
1016 GGGCCCC
EcoRI 518 GAATTC G^AATTC
1363 GAATTC
FauI 150 CCCGC CCCGC(4/6)
409 CCCGC
HaeII 22 GGCGCC [GA]GCGC^[CT]
1243 GGCGCC
Hin1I 22 GGCGCC G[GA]^CG[CT]C
1243 GGCGCC
HphI 298 GGTGA GGTGA(8/7)
1204 GGTGA
KasI 22 GGCGCC G^GCGCC
1243 GGCGCC
MaeI 17 CTAG C^TAG
338 CTAG
MslI 398 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1230 CACGGGAGTG
MspA1I 988 CAGCGG C[AC]G^C[GT]G
1386 CAGCTG
NarI 22 GGCGCC GG^CGCC
1243 GGCGCC
SfoI 22 GGCGCC GGC^GCC
1243 GGCGCC
SmlI 946 CTCGAG C^T[CT][GA]AG
1096 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BsgI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I