(Linear) (Six-base) MAPSORT of: KIBB5844.seq Check: 8981 from: 1 to: 1452 KIBB5844.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 758 1452 Size: 758 694 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 391 635 1426 1452 Size: 391 244 791 26 Fragments arranged by size: 791 391 244 26 AflIII A'CryG_T Cuts at: 0 440 1452 Size: 440 1012 AhdI GACnn_n'nnGTC Cuts at: 0 365 480 1452 Size: 365 115 972 Fragments arranged by size: 972 365 115 AleI CACnn'nnGTG Cuts at: 0 1234 1452 Size: 1234 218 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1183 1452 Size: 1183 269 ApaI G_GGCC'C Cuts at: 0 1020 1452 Size: 1020 432 ApoI r'AATT_y Cuts at: 0 518 1114 1295 1363 1452 Size: 518 596 181 68 89 Fragments arranged by size: 596 518 181 89 68 AvaI C'yCGr_G Cuts at: 0 93 253 844 946 1020 1452 Size: 93 160 591 102 74 432 Fragments arranged by size: 591 432 160 102 93 74 BanI G'GyrC_C Cuts at: 0 22 1243 1452 Size: 22 1221 209 Fragments arranged by size: 1221 209 22 BanII G_rGCy'C Cuts at: 0 238 1020 1137 1452 Size: 238 782 117 315 Fragments arranged by size: 782 315 238 117 BbeI G_GCGC'C Cuts at: 0 26 1247 1452 Size: 26 1221 205 Fragments arranged by size: 1221 205 26 BbvCI CC'TCA_GC Cuts at: 0 986 1452 Size: 986 466 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 858 892 1452 Size: 858 34 560 Fragments arranged by size: 858 560 34 BglI GCCn_nnn'nGGC Cuts at: 0 70 1157 1452 Size: 70 1087 295 Fragments arranged by size: 1087 295 70 Bme1580I G_kGCm'C Cuts at: 0 1020 1452 Size: 1020 432 BmgBI CAC'GTC Cuts at: 0 1428 1452 Size: 1428 24 BmrI ACTGGGnnnn_n' Cuts at: 0 392 1452 Size: 392 1060 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 642 1452 Size: 642 810 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 577 679 1340 1452 Size: 139 438 102 661 112 Fragments arranged by size: 661 438 139 112 102 Bpu10I CC'TnA_GC Cuts at: 0 832 986 1452 Size: 832 154 466 Fragments arranged by size: 832 466 154 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1117 1452 Size: 1117 335 BsaI GGTCTCn'nnnn_ Cuts at: 0 718 922 1416 1452 Size: 718 204 494 36 Fragments arranged by size: 718 494 204 36 BsaAI yAC'GTr Cuts at: 0 441 1452 Size: 441 1011 BsaHI Gr'CG_yC Cuts at: 0 23 1244 1452 Size: 23 1221 208 Fragments arranged by size: 1221 208 23 BsaWI w'CCGG_w Cuts at: 0 1129 1452 Size: 1129 323 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 951 1273 1452 Size: 951 322 179 Fragments arranged by size: 951 322 179 BseYI C'CCAG_C Cuts at: 0 632 1452 Size: 632 820 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 583 594 1452 Size: 85 498 11 858 Fragments arranged by size: 858 498 85 11 BsiEI CG_ry'CG Cuts at: 0 1014 1452 Size: 1014 438 BsiHKAI G_wGCw'C Cuts at: 0 260 1452 Size: 260 1192 Bsp1286I G_dGCh'C Cuts at: 0 238 260 1020 1137 1452 Size: 238 22 760 117 315 Fragments arranged by size: 760 315 238 117 22 BspEI T'CCGG_A Cuts at: 0 1129 1452 Size: 1129 323 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 758 1452 Size: 758 694 BsrBI CCG'CTC Cuts at: 0 153 1452 Size: 153 1299 BsrFI r'CCGG_y Cuts at: 0 836 1452 Size: 836 616 BsrGI T'GTAC_A Cuts at: 0 218 1452 Size: 218 1234 BstAPI GCAn_nnn'nTGC Cuts at: 0 531 561 1452 Size: 531 30 891 Fragments arranged by size: 891 531 30 BstEII G'GTnAC_C Cuts at: 0 159 1452 Size: 159 1293 BstXI CCAn_nnnn'nTGG Cuts at: 0 404 601 1236 1452 Size: 404 197 635 216 Fragments arranged by size: 635 404 216 197 BstYI r'GATC_y Cuts at: 0 920 1452 Size: 920 532 Bsu36I CC'TnA_GG Cuts at: 0 934 1153 1452 Size: 934 219 299 Fragments arranged by size: 934 299 219 BtgI C'CryG_G Cuts at: 0 1055 1229 1452 Size: 1055 174 223 Fragments arranged by size: 1055 223 174 BtsI GCAGTG_nn' Cuts at: 0 474 643 1452 Size: 474 169 809 Fragments arranged by size: 809 474 169 ClaI AT'CG_AT Cuts at: 0 740 1452 Size: 740 712 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 780 815 1452 Size: 780 35 637 Fragments arranged by size: 780 637 35 DrdI GACnn_nn'nnGTC Cuts at: 0 307 1452 Size: 307 1145 EaeI y'GGCC_r Cuts at: 0 1058 1211 1452 Size: 1058 153 241 Fragments arranged by size: 1058 241 153 EarI CTCTTCn'nnn_ Cuts at: 0 367 611 1304 1452 Size: 367 244 693 148 Fragments arranged by size: 693 367 244 148 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1452 Size: 19 1433 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 391 577 635 679 1340 1426 1452 Size: 139 252 186 58 44 661 86 26 Fragments arranged by size: 661 252 186 139 86 58 44 26 EcoO109I rG'GnC_Cy Cuts at: 0 543 1017 1452 Size: 543 474 435 Fragments arranged by size: 543 474 435 EcoRI G'AATT_C Cuts at: 0 518 1363 1452 Size: 518 845 89 Fragments arranged by size: 845 518 89 HaeII r_GCGC'y Cuts at: 0 26 1247 1452 Size: 26 1221 205 Fragments arranged by size: 1221 205 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 351 383 466 498 935 967 1092 1124 Size: 351 32 83 32 437 32 125 32 Cuts at: 1124 1452 Size: 328 Fragments arranged by size: 437 351 328 125 83 32 32 32 32 HincII GTy'rAC Cuts at: 0 359 765 1304 1452 Size: 359 406 539 148 Fragments arranged by size: 539 406 359 148 KasI G'GCGC_C Cuts at: 0 22 1243 1452 Size: 22 1221 209 Fragments arranged by size: 1221 209 22 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 815 1310 1452 Size: 815 495 142 Fragments arranged by size: 815 495 142 MscI TGG'CCA Cuts at: 0 1213 1452 Size: 1213 239 MslI CAynn'nnrTG Cuts at: 0 402 1234 1452 Size: 402 832 218 Fragments arranged by size: 832 402 218 MspA1I CmG'CkG Cuts at: 0 990 1388 1452 Size: 990 398 64 Fragments arranged by size: 990 398 64 NaeI GCC'GGC Cuts at: 0 838 1452 Size: 838 614 NarI GG'CG_CC Cuts at: 0 23 1244 1452 Size: 23 1221 208 Fragments arranged by size: 1221 208 23 NgoMIV G'CCGG_C Cuts at: 0 836 1452 Size: 836 616 NspI r_CATG'y Cuts at: 0 219 531 714 1452 Size: 219 312 183 738 Fragments arranged by size: 738 312 219 183 PfoI T'CCnGG_A Cuts at: 0 656 923 1452 Size: 656 267 529 Fragments arranged by size: 656 529 267 PpuMI rG'GwC_Cy Cuts at: 0 543 1452 Size: 543 909 PspOMI G'GGCC_C Cuts at: 0 1016 1452 Size: 1016 436 PstI C_TGCA'G Cuts at: 0 469 1452 Size: 469 983 PvuII CAG'CTG Cuts at: 0 1388 1452 Size: 1388 64 SapI GCTCTTCn'nnn_ Cuts at: 0 611 1452 Size: 611 841 ScaI AGT'ACT Cuts at: 0 178 1452 Size: 178 1274 SfcI C'TryA_G Cuts at: 0 465 1452 Size: 465 987 SfoI GGC'GCC Cuts at: 0 24 1245 1452 Size: 24 1221 207 Fragments arranged by size: 1221 207 24 SmaI CCC'GGG Cuts at: 0 95 1452 Size: 95 1357 SmlI C'TyrA_G Cuts at: 0 946 1096 1452 Size: 946 150 356 Fragments arranged by size: 946 356 150 StyI C'CwwG_G Cuts at: 0 200 1452 Size: 200 1252 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 175 1321 1452 Size: 175 1146 131 Fragments arranged by size: 1146 175 131 TatI w'GTAC_w Cuts at: 0 176 218 1382 1452 Size: 176 42 1164 70 Fragments arranged by size: 1164 176 70 42 XhoI C'TCGA_G Cuts at: 0 946 1452 Size: 946 506 XmaI C'CCGG_G Cuts at: 0 93 1452 Size: 93 1359 XmnI GAAnn'nnTTC Cuts at: 0 460 1452 Size: 460 992 Enzymes that do cut: AarI AcuI AflIII AhdI AleI AlfI ApaI ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PfoI PpuMI PspOMI PstI PvuII SapI ScaI SfcI SfoI SmaI SmlI StyI TaqII TatI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BlpI BmtI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrDI BssHII BssSI BstBI BstZ17I BtgZI DraI DraIII EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI ZraI