################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI ItaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI TseI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AfaI 568 GTAC GT^AC BanII 890 GAGCTC G[GA]GC[CT]^C BcnI 558 CCGGG CC^[GC]GG BglII 853 AGATCT A^GATCT BpmI 769 CTCCAG CTGGAG(16/14) BsaI 658 GAGACC GGTCTC(1/5) BsaWI 98 ACCGGA [AT]^CCGG[AT] BsmFI 626 GGGAC GGGAC(10/14) BsmI 88 GAATGC GAATGC(1/-1) BspHI 251 TCATGA T^CATGA BstAPI 943 GCAGTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC EaeI 555 TGGCCG [CT]^GGCC[GA] EarI 805 GAAGAG CTCTTC(1/4) EcoNI 369 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1057 GAATTC G^AATTC HgaI 268 GACGC GACGC(5/10) HindIII 967 AAGCTT A^AGCTT Ksp632I 805 GAAGAG CTCTTC(1/4) MaeII 111 ACGT A^CGT NciI 558 CCGGG CC^[GC]GG NdeI 983 CATATG CA^TATG PflMI 672 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 251 TCATGA T^CATGA RsaI 568 GTAC GT^AC SacI 890 GAGCTC GAGCT^C SfaNI 562 GCATC GCATC(5/9) SphI 78 GCATGC GCATG^C SspI 213 AATATT AAT^ATT Van91I 672 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AciI 366 CCGC CCGC(-3/-1) 919 CCGC AcsI 532 AAATTT [GA]^AATT[CT] 1057 GAATTC ApoI 532 AAATTT [GA]^AATT[CT] 1057 GAATTC AspHI 135 GAGCAC G[AT]GC[AT]^C 890 GAGCTC BfaI 427 CTAG C^TAG 512 CTAG BmyI 135 GAGCAC G[AGT]GC[ACT]^C 890 GAGCTC BsiHKAI 135 GAGCAC G[AT]GC[AT]^C 890 GAGCTC BsmAI 658 GAGAC GTCTC(1/5) 834 GTCTC Bsp1286I 135 GAGCAC G[AGT]GC[ACT]^C 890 GAGCTC DdeI 789 CTCAG C^T[ACGT]AG 1097 CTGAG DsaI 83 CCATGG C^C[GA][CT]GG 778 CCATGG EcoT14I 83 CCATGG C^C[AT][AT]GG 778 CCATGG HapII 99 CCGG C^CGG 558 CCGG HpaII 99 CCGG C^CGG 558 CCGG HphI 42 GGTGA GGTGA(8/7) 96 TCACC MaeI 427 CTAG C^TAG 512 CTAG MslI 246 CACACTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 939 CATGGCAGTG MspI 99 CCGG C^CGG 558 CCGG NcoI 83 CCATGG C^CATGG 778 CCATGG StyI 83 CCATGG C^C[AT][AT]GG 778 CCATGG TaqI 585 TCGA T^CGA 1038 TCGA Tsp45I 55 GTGAC ^GT[GC]AC 342 GTCAC TthHB8I 585 TCGA T^CGA 1038 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AluI AlwI AvaII BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DpnII EcoRII FokI HaeIII HinfI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfcI TfiI Tru9I Tsp509I TspRI XhoII