################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MluI MroI MslI MspA1I MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 191 CCGC CCGC(-3/-1) AcsI 552 GAATTC [GA]^AATT[CT] AflIII 215 ACACGT A^C[GA][CT]GT AlwNI 202 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 652 GGGCCC GGGCC^C ApoI 552 GAATTC [GA]^AATT[CT] AvaI 62 CCCGGG C^[CT]CG[GA]G AvaII 293 GGTCC G^G[AT]CC BalI 267 TGGCCA TGG^CCA BamHI 439 GGATCC G^GATCC BanI 275 GGTGCC G^G[CT][GA]CC BanII 652 GGGCCC G[GA]GC[CT]^C BfaI 103 CTAG C^TAG BpmI 401 CTCCAG CTGGAG(16/14) BsaAI 216 CACGTA [CT]AC^GT[GA] BsaI 662 GAGACC GGTCTC(1/5) BsaWI 158 TCCGGT [AT]^CCGG[AT] BseRI 318 CTCCTC GAGGAG(10/8) BsoBI 62 CCCGGG C^[CT]CG[GA]G DraII 651 AGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 510 GACAAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 267 TGGCCA [CT]^GGCC[GA] EcoO109I 651 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 552 GAATTC G^AATTC HgaI 75 GACGC GACGC(5/10) HphI 559 TCACC GGTGA(8/7) MaeI 103 CTAG C^TAG MaeII 217 ACGT A^CGT MboII 369 GAAGA GAAGA(8/7) MfeI 456 CAATTG C^AATTG MluNI 267 TGGCCA TGG^CCA MscI 267 TGGCCA TGG^CCA MseI 496 TTAA T^TAA MunI 456 CAATTG C^AATTG NdeI 480 CATATG CA^TATG NspI 466 GCATGC [GA]CATG^[CT] SexAI 350 ACCTGGT A^CC[AT]GGT SfaNI 306 GATGC GCATC(5/9) SmaI 62 CCCGGG CCC^GGG SmlI 116 CTCAAG C^T[CT][GA]AG SphI 466 GCATGC GCATG^C TaqI 660 TCGA T^CGA Tru9I 496 TTAA T^TAA TthHB8I 660 TCGA T^CGA XcmI 537 CCACCTAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 62 CCCGGG C^CCGGG XmaI 62 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AfaI 219 GTAC GT^AC 579 GTAC AluI 101 AGCT AG^CT 502 AGCT AspHI 226 GAGCAC G[AT]GC[AT]^C 285 GTGCTC BbvI 357 GCAGC GCAGC(8/12) 699 GCTGC BcnI 62 CCCGG CC^[GC]GG 63 CCGGG BsgI 199 GTGCAG GTGCAG(16/14) 355 GTGCAG BsiHKAI 226 GAGCAC G[AT]GC[AT]^C 285 GTGCTC BsiYI 240 CCAGGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 562 CCTGGAATTGG BslI 240 CCAGGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 562 CCTGGAATTGG BsmAI 662 GAGAC GTCTC(1/5) 399 GTCTC BsmFI 294 GTCCC GGGAC(10/14) 429 GTCCC BstF5I 236 CATCC GGATG(2/0) 87 GGATG BstYI 439 GGATCC [GA]^GATC[CT] 669 AGATCC Cac8I 173 GCCAGC GC[ACGT]^[ACGT]GC 466 GCATGC DpnII 440 GATC ^GATC 670 GATC Fnu4HI 357 GCAGC GC^[ACGT]GC 699 GCTGC FokI 236 CATCC GGATG(9/13) 87 GGATG HapII 63 CCGG C^CGG 159 CCGG HinfI 204 GATTC G^A[ACGT]TC 339 GATTC HpaII 63 CCGG C^CGG 159 CCGG ItaI 357 GCAGC GC^[ACGT]GC 699 GCTGC MboI 440 GATC ^GATC 670 GATC MflI 439 GGATCC [GA]^GATC[CT] 669 AGATCC MspI 63 CCGG C^CGG 159 CCGG NciI 62 CCCGG CC^[GC]GG 63 CCGGG NdeII 440 GATC ^GATC 670 GATC RsaI 219 GTAC GT^AC 579 GTAC Sau3AI 440 GATC ^GATC 670 GATC TfiI 204 GATTC G^A[AT]TC 339 GATTC TseI 357 GCAGC G^C[AT]GC 699 GCTGC TspRI 229 CACTG CA[GC]TG[ACGT][ACGT]^ 674 CACTG XhoII 439 GGATCC [GA]^GATC[CT] 669 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AlwI BmyI BsaJI Bsp1286I BsrI BssKI BstNI Cfr13I DdeI EcoRII HaeIII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI Tsp509I