Restriction map for
pFN21AE1700
(Circular) (Six-base) MAPSORT of: pFN21AE1700.seq Check: 6199 from: 1 to: 5443
pFN21AE1700 5443 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3253 278
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2786 1937
Size: 849 4594
Acc65I G'GTAC_C
Cuts at: 2764 2764
Size: 5443
AclI AA'CG_TT
Cuts at: 1563 3570 3943 1563
Size: 2007 373 3063
Fragments arranged by size:
3063 2007 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2087 2105 2393 3506 4890 5158 1632
Size: 455 18 288 1113 1384 268 1917
Fragments arranged by size:
1917 1384 1113 455 288 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5246
AflIII A'CryG_T
Cuts at: 1203 2220 4342 5319 1203
Size: 1017 2122 977 1327
Fragments arranged by size:
2122 1327 1017 977
AgeI A'CCGG_T
Cuts at: 4274 4274
Size: 5443
AhdI GACnn_n'nnGTC
Cuts at: 4172 4172
Size: 5443
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2375 1184
Size: 1191 4252
AlwNI CAG_nnn'CTG
Cuts at: 2212 3186 4758 2212
Size: 974 1572 2897
Fragments arranged by size:
2897 1572 974
ApaI G_GGCC'C
Cuts at: 2661 2661
Size: 5443
ApaLI G'TGCA_C
Cuts at: 3500 4656 3500
Size: 1156 4287
ApoI r'AATT_y
Cuts at: 1493 2557 2752 2978 3130 5239 1493
Size: 1064 195 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 226 195 152
AseI AT'TA_AT
Cuts at: 160 1032 3997 160
Size: 872 2965 1606
Fragments arranged by size:
2965 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2768 1870
Size: 73 124 701 4545
Fragments arranged by size:
4545 701 124 73
BamHI G'GATC_C
Cuts at: 2444 2773 3140 2444
Size: 329 367 4747
Fragments arranged by size:
4747 367 329
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2280 2764 4219 618
Size: 325 217 627 493 484 1455 1842
Fragments arranged by size:
1842 1455 627 493 484 325 217
BanII G_rGCy'C
Cuts at: 1391 2661 2762 1391
Size: 1270 101 4072
Fragments arranged by size:
4072 1270 101
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3634 3668 1903
Size: 34 1697 34 3678
Fragments arranged by size:
3678 1697 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3335 4545 3335
Size: 1210 4233
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5443
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4054 136
Size: 107 122 71 631 605 2382 1525
Fragments arranged by size:
2382 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5438 5438
Size: 5443
BlpI GC'TnA_GC
Cuts at: 2860 2860
Size: 5443
Bme1580I G_kGCm'C
Cuts at: 1790 2661 3504 4660 1790
Size: 871 843 1156 2573
Fragments arranged by size:
2573 1156 871 843
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 2562 2655
Size: 416 53 83 399 24 819 326 93
Cuts at: 2655 3174 4132 442
Size: 519 958 1753
Fragments arranged by size:
1753 958 819 519 416 399 326 93
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5443
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2341 2421 2341
Size: 80 5363
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2391 4103 1239
Size: 1152 1712 2579
Fragments arranged by size:
2579 1712 1152
Bpu10I CC'TnA_GC
Cuts at: 2631 2631
Size: 5443
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2106 3568 4433 4731 2106
Size: 1462 865 298 2818
Fragments arranged by size:
2818 1462 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2661 4106 882
Size: 617 1162 1445 2219
Fragments arranged by size:
2219 1445 1162 617
BsaAI yAC'GTr
Cuts at: 493 1204 2223 3234 493
Size: 711 1019 1011 2702
Fragments arranged by size:
2702 1019 1011 711
BsaBI GATnn'nnATC
Cuts at: 5437 5437
Size: 5443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3250 3632 4267 275
Size: 53 83 186 936 1717 382 635 1451
Fragments arranged by size:
1717 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2163 3876 4274 4548 4695 5106 2163
Size: 1713 398 274 147 411 2500
Fragments arranged by size:
2500 1713 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5218
Fragments arranged by size:
5218 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2314 1186
Size: 1128 4315
BseYI C'CCAG_C
Cuts at: 4646 5142 4646
Size: 496 4947
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2225 2381 1163
Size: 573 489 156 4225
Fragments arranged by size:
4225 573 489 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2893 3654 4682 1540
Size: 275 186 892 761 1028 2301
Fragments arranged by size:
2301 1028 892 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2235 2294 3504 3589 4660 2235
Size: 59 1210 85 1071 3018
Fragments arranged by size:
3018 1210 1071 85 59
BsmI GAATG_Cn'
Cuts at: 2954 3047 2954
Size: 93 5350
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2235 2294 2661 2762 3504 3589 4660
Size: 399 445 59 367 101 742 85 1071
Cuts at: 4660 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 445 399 367 101 85
59
BspEI T'CCGG_A
Cuts at: 5106 5106
Size: 5443
BspHI T'CATG_A
Cuts at: 3332 5062 3332
Size: 1730 3713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2798 844
Size: 1954 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3330 1394
Size: 429 1507 3507
Fragments arranged by size:
3507 1507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3938 4112 65
Size: 1183 2690 174 1396
Fragments arranged by size:
2690 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4087 4274 1700
Size: 110 2277 187 2869
Fragments arranged by size:
2869 2277 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5443
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5443
BssSI C'ACGA_G
Cuts at: 3503 4515 3503
Size: 1012 4431
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5443
BstBI TT'CG_AA
Cuts at: 4247 4247
Size: 5443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5443
BstYI r'GATC_y
Cuts at: 1972 2444 2674 2773 3140 3525 3542 4994 5080
Size: 472 230 99 367 385 17 1452 86
Cuts at: 5080 5092 5438 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 472 385 367 346 230 99
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3952
Fragments arranged by size:
3952 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4704
Fragments arranged by size:
4704 683 56
BtsI GCAGTG_nn'
Cuts at: 2959 3753 3773 2959
Size: 794 20 4629
Fragments arranged by size:
4629 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5408
DraI TTT'AAA
Cuts at: 2747 2747
Size: 5443
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2521 4450 809
Size: 507 1205 1929 1802
Fragments arranged by size:
1929 1802 1205 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2272 2890 3779
Size: 54 1425 50 275 18 442 618 889
Cuts at: 3779 5408 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 618 442 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2890 1537
Size: 275 1078 4090
Fragments arranged by size:
4090 1078 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3373 1353
Size: 2020 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2185 4030 4404 4550 387
Size: 1064 138 596 1845 374 146 1280
Fragments arranged by size:
1845 1280 1064 596 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2105 2391 2393 3506 4103 4890
Size: 393 455 18 286 2 1113 597 787
Cuts at: 4890 5158 1239
Size: 268 1524
Fragments arranged by size:
1524 1113 787 597 455 393 286 268
18 2
EcoO109I rG'GnC_Cy
Cuts at: 2657 2657
Size: 5443
EcoRI G'AATT_C
Cuts at: 2557 2752 3130 2557
Size: 195 378 4870
Fragments arranged by size:
4870 378 195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2208 2208
Size: 5443
FspI TGC'GCA
Cuts at: 3949 3949
Size: 5443
HaeII r_GCGC'y
Cuts at: 1678 4590 1678
Size: 2912 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4084 4116 4158 4190 1373
Size: 32 135 32 2512 32 42 32 2626
Fragments arranged by size:
2626 2512 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2787 3033 669
Size: 988 281 849 246 3079
Fragments arranged by size:
3079 988 849 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5443
HpaI GTT'AAC
Cuts at: 3033 3033
Size: 5443
KpnI G_GTAC'C
Cuts at: 2768 2768
Size: 5443
MfeI C'AATT_G
Cuts at: 2461 3042 2461
Size: 581 4862
MluI A'CGCG_T
Cuts at: 5319 5319
Size: 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 4557 4741 1305
Size: 765 2487 184 2007
Fragments arranged by size:
2487 2007 765 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2274 10
Size: 54 1425 343 442 3179
Fragments arranged by size:
3179 1425 442 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3401 3760 3919 518
Size: 648 2235 359 159 2042
Fragments arranged by size:
2235 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2859 3536 4684 4929 1606
Size: 1253 677 1148 245 2120
Fragments arranged by size:
2120 1253 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3952
Fragments arranged by size:
3952 939 552
NdeI CA'TA_TG
Cuts at: 387 2486 387
Size: 2099 3344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5443
NotI GC'GGCC_GC
Cuts at: 2890 2890
Size: 5443
NspI r_CATG'y
Cuts at: 1132 1229 2475 2801 4346 1132
Size: 97 1246 326 1545 2229
Fragments arranged by size:
2229 1545 1246 326 97
PasI CC'CwG_GG
Cuts at: 1348 2245 1348
Size: 897 4546
PciI A'CATG_T
Cuts at: 4342 4342
Size: 5443
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5443
PfoI T'CCnGG_A
Cuts at: 2424 2424
Size: 5443
PmeI GTTT'AAAC
Cuts at: 2747 2747
Size: 5443
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3512 3544 5025 5057 3512
Size: 32 1481 32 3898
Fragments arranged by size:
3898 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5443
PsiI TTA'TAA
Cuts at: 3013 3013
Size: 5443
PspOMI G'GGCC_C
Cuts at: 2657 2657
Size: 5443
PstI C_TGCA'G
Cuts at: 830 2795 830
Size: 1965 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5443
SalI G'TCGA_C
Cuts at: 1936 2785 1936
Size: 849 4594
SbfI CC_TGCA'GG
Cuts at: 2795 2795
Size: 5443
ScaI AGT'ACT
Cuts at: 3691 5403 3691
Size: 1712 3731
SexAI A'CCwGG_T
Cuts at: 2355 2355
Size: 5443
SfcI C'TryA_G
Cuts at: 826 1045 2791 3926 4607 4798 826
Size: 219 1746 1135 681 191 1471
Fragments arranged by size:
1746 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5443
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5443
SmaI CCC'GGG
Cuts at: 1872 2069 2770 1872
Size: 197 701 4545
Fragments arranged by size:
4545 701 197
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2121 3547 4448 4710 820
Size: 197 926 178 1426 901 262 1553
Fragments arranged by size:
1553 1426 926 901 262 197 178
SnaBI TAC'GTA
Cuts at: 493 3234 493
Size: 2741 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5443
SphI G_CATG'C
Cuts at: 1132 2475 2801 1132
Size: 1343 326 3774
Fragments arranged by size:
3774 1343 326
SspI AAT'ATT
Cuts at: 5 52 3367 5
Size: 47 3315 2081
Fragments arranged by size:
3315 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5443
StyI C'CwwG_G
Cuts at: 513 1065 2004 2882 513
Size: 552 939 878 3074
Fragments arranged by size:
3074 939 878 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3470 3487 3640 3825 4270 325
Size: 1255 1890 17 153 185 445 1498
Fragments arranged by size:
1890 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3689 5401 96
Size: 274 80 33 51 1443 1712 1712 138
Fragments arranged by size:
1712 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2225 2257 5403 5435 2225
Size: 32 3146 32 2233
Fragments arranged by size:
3146 2233 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5443
XbaI T'CTAG_A
Cuts at: 2779 2779
Size: 5443
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2549 2549
Size: 5443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5443
XmaI C'CCGG_G
Cuts at: 1870 2067 2768 1870
Size: 197 701 4545
Fragments arranged by size:
4545 701 197
XmnI GAAnn'nnTTC
Cuts at: 3572 3572
Size: 5443
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3251 276
Size: 53 83 186 936 1717 2468
Fragments arranged by size:
2468 1717 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI