################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## Acc65I AccI AccIII AcsI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AseI AsnI Asp700I Asp718I AspI AvaII AviII AvrII BanII BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BsaWI BseAI BsiEI BsiWI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeI MaeIII MfeI MluI MroI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 731 GACGTC GACGT^C AclI 289 AACGTT AA^CGTT AhdI 726 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 322 CCTGAGG CC^T[ACGT]AGG AscI 163 GGCGCGCC GG^CGCGCC AspEI 726 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 301 GTGCTC G[AT]GC[AT]^C BamHI 192 GGATCC G^GATCC BbeI 31 GGCGCC GGCGC^C BbrPI 55 CACGTG CAC^GTG BbvCI 476 CCTCAGC CCTCAGC(-5/-2) BlpI 103 GCTGAGC GC^T[ACGT]AGC Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC BsaAI 55 CACGTG [CT]AC^GT[GA] BsaBI 193 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 754 GTGCAG GTGCAG(16/14) BsiHKAI 301 GTGCTC G[AT]GC[AT]^C BsmBI 733 CGTCTC CGTCTC(1/5) BsmI 242 GCATTC GAATGC(1/-1) BsrBI 106 GAGCGG CCGCTC(-3/-3) BssSI 590 CACGAG CACGAG(-5/-1) BstYI 192 GGATCC [GA]^GATC[CT] Bsu36I 322 CCTGAGG CC^T[ACGT]AGG CelII 103 GCTGAGC GC^T[ACGT]AGC Eam1105I 726 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 322 CCTGAGG CC^T[ACGT]AGG EcoT14I 176 CCAAGG C^C[AT][AT]GG FauI 719 CCCGC CCCGC(4/6) HinfI 612 GAATC G^A[ACGT]TC KasI 31 GGCGCC G^GCGCC KspI 159 CCGCGG CCGC^GG MamI 193 GATCCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 192 GGATCC [GA]^GATC[CT] MseI 206 TTAA T^TAA MspA1I 159 CCGCGG C[AC]G^C[GT]G NarI 31 GGCGCC GG^CGCC PmaCI 55 CACGTG CAC^GTG PmlI 55 CACGTG CAC^GTG Psp1406I 289 AACGTT AA^CGTT SacII 159 CCGCGG CCGC^GG SfoI 31 GGCGCC GGC^GCC StyI 176 CCAAGG C^C[AT][AT]GG TfiI 612 GAATC G^A[AT]TC Tru9I 206 TTAA T^TAA Tsp509I 522 AATT ^AATT XhoII 192 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 31 GGCGCC G[GA]^CG[CT]C 731 GACGTC AvaI 153 CCCGGG C^[CT]CG[GA]G 380 CCCGGG BalI 586 TGGCCA TGG^CCA 605 TGGCCA BanI 31 GGCGCC G^G[CT][GA]CC 313 GGCACC BbsI 432 GAAGAC GAAGAC(2/6) 184 GTCTTC BmyI 301 GTGCTC G[AGT]GC[ACT]^C 312 GGGCAC BpuAI 432 GAAGAC GAAGAC(2/6) 184 GTCTTC BsaHI 31 GGCGCC G[GA]^CG[CT]C 731 GACGTC BseRI 474 CTCCTC GAGGAG(10/8) 349 GAGGAG BsoBI 153 CCCGGG C^[CT]CG[GA]G 380 CCCGGG Bsp1286I 301 GTGCTC G[AGT]GC[ACT]^C 312 GGGCAC BsrI 727 ACTGG ACTGG(1/-1) 255 CCAGT BstNI 345 CCTGG CC^[AT]GG 358 CCTGG DpnII 12 GATC ^GATC 193 GATC EcoNI 171 CCTCGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 628 CCTTTTGGAGG EcoRII 345 CCTGG ^CC[AT]GG 358 CCTGG HaeII 31 GGCGCC [GA]GCGC^[CT] 73 GGCGCT Hin1I 31 GGCGCC G[GA]^CG[CT]C 731 GACGTC MboI 12 GATC ^GATC 193 GATC MboII 432 GAAGA GAAGA(8/7) 185 TCTTC MluNI 586 TGGCCA TGG^CCA 605 TGGCCA MscI 586 TGGCCA TGG^CCA 605 TGGCCA MvaI 345 CCTGG CC^[AT]GG 358 CCTGG NdeII 12 GATC ^GATC 193 GATC NlaIII 318 CATG CATG^ 609 CATG Sau3AI 12 GATC ^GATC 193 GATC SmaI 153 CCCGGG CCC^GGG 380 CCCGGG XmaCI 153 CCCGGG C^CCGGG 380 CCCGGG XmaI 153 CCCGGG C^CCGGG 380 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AccII AciI AfaI AluI AlwI BbvI BcnI BpmI BsaI BsaJI BsiYI BslI BsmAI BsrDI BssHII BssKI BstF5I BstUI Cac8I CfoI Cfr13I DdeI DsaI EaeI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MnlI MspI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI SfaNI TseI XcmI