(Circular) (Six-base) MAPSORT of: pFN21AE1503.seq Check: 5525 from: 1 to: 5476 pFN21AE1503 5476 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2740 3286 278 Size: 53 83 186 936 1204 546 2468 Fragments arranged by size: 2468 1204 936 546 186 83 53 AccI GT'mk_AC Cuts at: 1937 2819 1937 Size: 882 4594 Acc65I G'GTAC_C Cuts at: 2797 2797 Size: 5476 AclI AA'CG_TT Cuts at: 1563 2295 2775 3603 3976 1563 Size: 732 480 828 373 3063 Fragments arranged by size: 3063 828 732 480 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3539 4923 5191 1632 Size: 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5279 AflIII A'CryG_T Cuts at: 1203 4375 5352 1203 Size: 3172 977 1327 Fragments arranged by size: 3172 1327 977 AgeI A'CCGG_T Cuts at: 4307 4307 Size: 5476 AhdI GACnn_n'nnGTC Cuts at: 2736 4205 2736 Size: 1469 4007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5476 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5476 AlwNI CAG_nnn'CTG Cuts at: 3219 4791 3219 Size: 1572 3904 ApaLI G'TGCA_C Cuts at: 3533 4689 3533 Size: 1156 4320 ApoI r'AATT_y Cuts at: 1493 2785 3011 3163 5272 1493 Size: 1292 226 152 2109 1697 Fragments arranged by size: 2109 1697 1292 226 152 AscI GG'CGCG_CC Cuts at: 2169 2169 Size: 5476 AseI AT'TA_AT Cuts at: 160 1032 4030 160 Size: 872 2998 1606 Fragments arranged by size: 2998 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 AvaI C'yCGr_G Cuts at: 1870 1943 2158 2385 2801 1870 Size: 73 215 227 416 4545 Fragments arranged by size: 4545 416 227 215 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2325 2358 2325 Size: 33 5443 BamHI G'GATC_C Cuts at: 2197 2806 3173 2197 Size: 609 367 4500 Fragments arranged by size: 4500 609 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2318 2797 4252 618 Size: 325 217 627 249 282 479 1455 1842 Fragments arranged by size: 1842 1455 627 479 325 282 249 217 BanII G_rGCy'C Cuts at: 1391 2795 1391 Size: 1404 4072 BbeI G_GCGC'C Cuts at: 2040 2040 Size: 5476 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2182 2444 928 Size: 971 283 262 3960 Fragments arranged by size: 3960 971 283 262 BbvCI CC'TCA_GC Cuts at: 2482 2482 Size: 5476 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3667 3701 1903 Size: 34 1730 34 3678 Fragments arranged by size: 3678 1730 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3368 4578 3368 Size: 1210 4266 BclI T'GATC_A Cuts at: 1548 1548 Size: 5476 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4087 136 Size: 107 122 71 631 605 2415 1525 Fragments arranged by size: 2415 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5471 5471 Size: 5476 BlpI GC'TnA_GC Cuts at: 2109 2893 2109 Size: 784 4692 Bme1580I G_kGCm'C Cuts at: 1790 2321 3537 4693 1790 Size: 531 1216 1156 2573 Fragments arranged by size: 2573 1216 1156 531 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5476 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3207 4165 442 Size: 416 53 83 399 24 1790 958 1753 Fragments arranged by size: 1790 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5476 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2372 2385 2535 4136 1239 Size: 1133 13 150 1601 2579 Fragments arranged by size: 2579 1601 1133 150 13 Bpu10I CC'TnA_GC Cuts at: 2482 2482 Size: 5476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3601 4466 4764 3601 Size: 865 298 4313 Fragments arranged by size: 4313 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2361 2423 2717 4139 882 Size: 617 862 62 294 1422 2219 Fragments arranged by size: 2219 1422 862 617 294 62 BsaAI yAC'GTr Cuts at: 493 1204 2062 3267 493 Size: 711 858 1205 2702 Fragments arranged by size: 2702 1205 858 711 BsaBI GATnn'nnATC Cuts at: 2202 5470 2202 Size: 3268 2208 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2737 3283 3665 Size: 53 83 186 936 504 700 546 382 Cuts at: 3665 4300 275 Size: 635 1451 Fragments arranged by size: 1451 936 700 635 546 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3909 4307 4581 4728 5139 3909 Size: 398 274 147 411 4246 Fragments arranged by size: 4246 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5251 Fragments arranged by size: 5251 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2470 1186 Size: 1183 101 4192 Fragments arranged by size: 4192 1183 101 BseYI C'CCAG_C Cuts at: 2286 4679 5175 2286 Size: 2393 496 2587 Fragments arranged by size: 2587 2393 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2780 1163 Size: 573 1044 3859 Fragments arranged by size: 3859 1044 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2926 3687 4715 1540 Size: 275 186 925 761 1028 2301 Fragments arranged by size: 2301 1028 925 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2310 3537 3622 4693 2310 Size: 1227 85 1071 3093 Fragments arranged by size: 3093 1227 1071 85 BsmI GAATG_Cn' Cuts at: 2247 2987 3080 2247 Size: 740 93 4643 Fragments arranged by size: 4643 740 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2744 2744 Size: 5476 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2321 2795 3537 3622 4693 1391 Size: 399 520 11 474 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 520 474 399 85 11 BspEI T'CCGG_A Cuts at: 5139 5139 Size: 5476 BspHI T'CATG_A Cuts at: 3365 5095 3365 Size: 1730 3746 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2831 844 Size: 1987 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2113 3363 1394 Size: 429 290 1250 3507 Fragments arranged by size: 3507 1250 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 2232 2308 2715 3971 4145 65 Size: 1183 984 76 407 1256 174 1396 Fragments arranged by size: 1396 1256 1183 984 407 174 76 BsrFI r'CCGG_y Cuts at: 1700 1810 4120 4307 1700 Size: 110 2310 187 2869 Fragments arranged by size: 2869 2310 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5476 BssHII G'CGCG_C Cuts at: 1925 2055 2084 2092 2169 1925 Size: 130 29 8 77 5232 Fragments arranged by size: 5232 130 77 29 8 BssSI C'ACGA_G Cuts at: 2595 3536 4548 2595 Size: 941 1012 3523 Fragments arranged by size: 3523 1012 941 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5476 BstBI TT'CG_AA Cuts at: 4280 4280 Size: 5476 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5476 BstYI r'GATC_y Cuts at: 1972 2197 2806 3173 3558 3575 5027 5113 5125 Size: 225 609 367 385 17 1452 86 12 Cuts at: 5125 5471 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 609 385 367 346 225 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2328 2328 Size: 5476 BtgI C'CryG_G Cuts at: 513 1065 2004 2020 2097 2164 2371 513 Size: 552 939 16 77 67 207 3618 Fragments arranged by size: 3618 939 552 207 77 67 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4737 Fragments arranged by size: 4737 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3786 3806 2992 Size: 794 20 4662 Fragments arranged by size: 4662 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5476 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5441 DraI TTT'AAA Cuts at: 2780 2780 Size: 5476 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4483 809 Size: 507 3167 1802 Fragments arranged by size: 3167 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2521 2551 2591 Size: 54 1425 50 275 18 691 30 40 Cuts at: 2591 2610 2698 2923 3812 5441 8 Size: 19 88 225 889 1629 43 Fragments arranged by size: 1629 1425 889 691 275 225 88 54 50 43 40 30 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2923 1537 Size: 275 1111 4090 Fragments arranged by size: 4090 1111 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3406 1353 Size: 2053 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4063 4437 4583 387 Size: 1064 138 2474 374 146 1280 Fragments arranged by size: 2474 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2372 2385 2535 3539 4136 4923 5191 Size: 393 740 13 150 1004 597 787 268 Cuts at: 5191 1239 Size: 1524 Fragments arranged by size: 1524 1004 787 740 597 393 268 150 13 EcoNI CCTnn'n_nnAGG Cuts at: 2180 2637 2180 Size: 457 5019 EcoRI G'AATT_C Cuts at: 2785 3163 2785 Size: 378 5098 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2206 2206 Size: 5476 FspI TGC'GCA Cuts at: 3982 3982 Size: 5476 HaeII r_GCGC'y Cuts at: 1678 2040 2082 4623 1678 Size: 362 42 2541 2531 Fragments arranged by size: 2541 2531 362 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2722 2754 4117 4149 4191 Size: 32 135 32 1150 32 1363 32 42 Cuts at: 4191 4223 1373 Size: 32 2626 Fragments arranged by size: 2626 1363 1150 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2820 3066 669 Size: 988 281 882 246 3079 Fragments arranged by size: 3079 988 882 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5476 HpaI GTT'AAC Cuts at: 3066 3066 Size: 5476 KasI G'GCGC_C Cuts at: 2036 2036 Size: 5476 KpnI G_GTAC'C Cuts at: 2801 2801 Size: 5476 MfeI C'AATT_G Cuts at: 3075 3075 Size: 5476 MluI A'CGCG_T Cuts at: 5352 5352 Size: 5476 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4590 4774 1305 Size: 3285 184 2007 Fragments arranged by size: 3285 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2593 2612 10 Size: 54 1425 343 761 19 2874 Fragments arranged by size: 2874 1425 761 343 54 19 MslI CAynn'nnrTG Cuts at: 518 1166 3434 3793 3952 518 Size: 648 2268 359 159 2042 Fragments arranged by size: 2268 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2166 2892 3569 4717 4962 1606 Size: 560 726 677 1148 245 2120 Fragments arranged by size: 2120 1148 726 677 560 245 NarI GG'CG_CC Cuts at: 2037 2037 Size: 5476 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3985 Fragments arranged by size: 3985 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5476 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5476 NotI GC'GGCC_GC Cuts at: 2923 2923 Size: 5476 NspI r_CATG'y Cuts at: 1132 1229 2834 4379 1132 Size: 97 1605 1545 2229 Fragments arranged by size: 2229 1605 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5476 PciI A'CATG_T Cuts at: 4375 4375 Size: 5476 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5476 PfoI T'CCnGG_A Cuts at: 2193 2193 Size: 5476 PmeI GTTT'AAAC Cuts at: 2780 2780 Size: 5476 PmlI CAC'GTG Cuts at: 2062 2062 Size: 5476 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3545 3577 5058 5090 3545 Size: 32 1481 32 3931 Fragments arranged by size: 3931 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5476 PsiI TTA'TAA Cuts at: 3046 3046 Size: 5476 PstI C_TGCA'G Cuts at: 830 2828 830 Size: 1998 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5476 SacII CC_GC'GG Cuts at: 2167 2167 Size: 5476 SalI G'TCGA_C Cuts at: 1936 2818 1936 Size: 882 4594 SbfI CC_TGCA'GG Cuts at: 2828 2828 Size: 5476 ScaI AGT'ACT Cuts at: 3724 5436 3724 Size: 1712 3764 SfcI C'TryA_G Cuts at: 826 1045 2824 3959 4640 4831 826 Size: 219 1779 1135 681 191 1471 Fragments arranged by size: 1779 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2038 2038 Size: 5476 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5476 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5476 SmaI CCC'GGG Cuts at: 1872 2160 2387 2803 1872 Size: 288 227 416 4545 Fragments arranged by size: 4545 416 288 227 SmlI C'TyrA_G Cuts at: 820 1017 1943 3580 4481 4743 820 Size: 197 926 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3267 493 Size: 2774 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5476 SphI G_CATG'C Cuts at: 1132 2834 1132 Size: 1702 3774 SrfI GCCC'GGGC Cuts at: 2387 2387 Size: 5476 SspI AAT'ATT Cuts at: 5 52 3400 5 Size: 47 3348 2081 Fragments arranged by size: 3348 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5476 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2915 513 Size: 552 939 177 734 3074 Fragments arranged by size: 3074 939 734 552 177 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3503 3520 3673 3858 4303 325 Size: 1255 1923 17 153 185 445 1498 Fragments arranged by size: 1923 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3722 5434 96 Size: 274 80 33 51 1443 1745 1712 138 Fragments arranged by size: 1745 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5436 5468 5436 Size: 32 5444 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5476 XbaI T'CTAG_A Cuts at: 2812 2812 Size: 5476 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2294 2494 2620 2294 Size: 200 126 5150 Fragments arranged by size: 5150 200 126 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5476 XmaI C'CCGG_G Cuts at: 1870 2158 2385 2801 1870 Size: 288 227 416 4545 Fragments arranged by size: 4545 416 288 227 XmnI GAAnn'nnTTC Cuts at: 3605 3605 Size: 5476 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2738 3284 276 Size: 53 83 186 936 1204 546 2468 Fragments arranged by size: 2468 1204 936 546 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SwaI