################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvaI AvaII AviII AvrII BamHI BanI BbeI BbsI BbvCI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MaeIII MamI MboII MfeI MluI MroI MseI MslI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Alw44I 152 GTGCAC G^TGCAC ApaI 7 GGGCCC GGGCC^C ApaLI 152 GTGCAC G^TGCAC AspHI 152 GTGCAC G[AT]GC[AT]^C AspI 408 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 35 TGGCCA TGG^CCA BbrPI 291 CACGTG CAC^GTG BcgI 236 GCAAGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 37 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 377 AGATCT A^GATCT BpmI 78 CTCCAG CTGGAG(16/14) BsaAI 291 CACGTG [CT]AC^GT[GA] BsiEI 71 CGGCCG CG[GA][CT]^CG BsiHKAI 152 GTGCAC G[AT]GC[AT]^C BsmFI 83 GTCCC GGGAC(10/14) BsmI 361 GCATTC GAATGC(1/-1) BspLU11I 197 ACATGT A^CATGT BspMI 219 GCAGGT ACCTGC(4/8) BsrFI 73 GCCGGC [GA]^CCGG[CT] BstAPI 219 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 39 CATCC GGATG(2/0) BstYI 377 AGATCT [GA]^GATC[CT] Cfr10I 73 GCCGGC [GA]^CCGG[CT] DrdI 160 GACTTTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 71 CGGCCG C^GGCCG EclXI 71 CGGCCG C^GGCCG Eco52I 71 CGGCCG C^GGCCG FokI 39 CATCC GGATG(9/13) HphI 255 GGTGA GGTGA(8/7) KspI 68 CCGCGG CCGC^GG MflI 377 AGATCT [GA]^GATC[CT] MluNI 35 TGGCCA TGG^CCA MscI 35 TGGCCA TGG^CCA NaeI 73 GCCGGC GCC^GGC NcoI 116 CCATGG C^CATGG NgoMIV 73 GCCGGC G^CCGGC NspI 197 ACATGT [GA]CATG^[CT] PflFI 408 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 291 CACGTG CAC^GTG PmlI 291 CACGTG CAC^GTG PstI 217 CTGCAG CTGCA^G SacII 68 CCGCGG CCGC^GG SfcI 217 CTGCAG C^T[GA][CT]AG SfiI 36 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 415 TCGA T^CGA TspRI 155 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 408 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 415 TCGA T^CGA XcmI 38 CCATCCTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 377 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 121 GACGCC G[GA]^CG[CT]C 433 GACGCC AflIII 197 ACATGT A^C[GA][CT]GT 292 ACGTGT AluI 249 AGCT AG^CT 278 AGCT Asp700I 258 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 357 GAAAGCATTC BanII 7 GGGCCC G[GA]GC[CT]^C 63 GAGCCC BbvI 24 GCTGC GCAGC(8/12) 216 GCTGC BcnI 5 CCGGG CC^[GC]GG 85 CCCGG BsaHI 121 GACGCC G[GA]^CG[CT]C 433 GACGCC BseRI 133 GAGGAG GAGGAG(10/8) 136 GAGGAG BsrI 156 ACTGG ACTGG(1/-1) 80 CCAGT DpnII 243 GATC ^GATC 378 GATC DraII 7 GGGCCCC [GA]G^G[ACGT]CC[CT] 111 AGGCCCC EcoO109I 7 GGGCCCC [GA]G^G[ACGT]CC[CT] 111 AGGCCCC EcoT14I 116 CCATGG C^C[AT][AT]GG 391 CCTTGG HgaI 121 GACGC GACGC(5/10) 433 GACGC Hin1I 121 GACGCC G[GA]^CG[CT]C 433 GACGCC MaeII 272 ACGT A^CGT 292 ACGT MboI 243 GATC ^GATC 378 GATC MspA1I 22 CCGCTG C[AC]G^C[GT]G 68 CCGCGG NciI 5 CCGGG CC^[GC]GG 85 CCCGG NdeII 243 GATC ^GATC 378 GATC Sau3AI 243 GATC ^GATC 378 GATC StyI 116 CCATGG C^C[AT][AT]GG 391 CCTTGG TseI 24 GCTGC G^C[AT]GC 216 GCTGC XmnI 258 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 357 GAAAGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AfaI BmyI BsaJI BsiYI BslI Bsp1286I BssHII BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI NlaIII NlaIV RsaI Sau96I ScrFI