(Circular) (Six-base) MAPSORT of: pFN21AE2270.seq Check: 1331 from: 1 to: 5143 pFN21AE2270 5143 bp With 182 enzymes: SgfI * August 16, 2010 14:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2215 2215 Size: 5143 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2953 278 Size: 53 83 186 936 1417 2468 Fragments arranged by size: 2468 1417 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2486 1937 Size: 549 4594 Acc65I G'GTAC_C Cuts at: 2464 2464 Size: 5143 AclI AA'CG_TT Cuts at: 1563 3270 3643 1563 Size: 1707 373 3063 Fragments arranged by size: 3063 1707 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3206 4590 4858 1632 Size: 1574 1384 268 1917 Fragments arranged by size: 1917 1574 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4946 AflIII A'CryG_T Cuts at: 1203 2202 2297 4042 5019 1203 Size: 999 95 1745 977 1327 Fragments arranged by size: 1745 1327 999 977 95 AgeI A'CCGG_T Cuts at: 3974 3974 Size: 5143 AhdI GACnn_n'nnGTC Cuts at: 3872 3872 Size: 5143 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5143 AlwNI CAG_nnn'CTG Cuts at: 2886 4458 2886 Size: 1572 3571 ApaI G_GGCC'C Cuts at: 2016 2016 Size: 5143 ApaLI G'TGCA_C Cuts at: 2157 3200 4356 2157 Size: 1043 1156 2944 Fragments arranged by size: 2944 1156 1043 ApoI r'AATT_y Cuts at: 1493 2452 2678 2830 4939 1493 Size: 959 226 152 2109 1697 Fragments arranged by size: 2109 1697 959 226 152 AseI AT'TA_AT Cuts at: 160 1032 3697 160 Size: 872 2665 1606 Fragments arranged by size: 2665 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5143 AvaI C'yCGr_G Cuts at: 1870 1943 2468 1870 Size: 73 525 4545 Fragments arranged by size: 4545 525 73 BamHI G'GATC_C Cuts at: 2473 2840 2473 Size: 367 4776 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2464 3919 618 Size: 325 217 627 677 1455 1842 Fragments arranged by size: 1842 1455 677 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2072 2462 1391 Size: 625 56 390 4072 Fragments arranged by size: 4072 625 390 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2230 2264 3334 3368 1903 Size: 34 293 34 1070 34 3678 Fragments arranged by size: 3678 1070 293 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3035 4245 3035 Size: 1210 3933 BclI T'GATC_A Cuts at: 1548 1548 Size: 5143 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 3754 136 Size: 107 122 71 631 605 376 1706 1525 Fragments arranged by size: 1706 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 2382 5138 2382 Size: 2756 2387 BlpI GC'TnA_GC Cuts at: 2560 2560 Size: 5143 Bme1580I G_kGCm'C Cuts at: 1790 2016 2161 2408 3204 4360 1790 Size: 226 145 247 796 1156 2573 Fragments arranged by size: 2573 1156 796 247 226 145 BmgBI CAC'GTC Cuts at: 1318 2278 1318 Size: 960 4183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2874 3832 442 Size: 416 53 83 399 24 1457 958 1753 Fragments arranged by size: 1753 1457 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5143 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2068 3803 1239 Size: 829 1735 2579 Fragments arranged by size: 2579 1735 829 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3268 4133 4431 3268 Size: 865 298 3980 Fragments arranged by size: 3980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3806 882 Size: 617 2307 2219 Fragments arranged by size: 2307 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2298 2934 493 Size: 711 1094 636 2702 Fragments arranged by size: 2702 1094 711 636 BsaBI GATnn'nnATC Cuts at: 5137 5137 Size: 5143 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2127 2439 2950 3332 Size: 53 83 186 936 594 312 511 382 Cuts at: 3332 3967 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 594 511 382 312 186 83 53 BsaWI w'CCGG_w Cuts at: 3576 3974 4248 4395 4806 3576 Size: 398 274 147 411 3913 Fragments arranged by size: 3913 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4918 Fragments arranged by size: 4918 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2156 1186 Size: 967 3 4173 Fragments arranged by size: 4173 967 3 BseYI C'CCAG_C Cuts at: 2255 2284 2310 4346 4842 2255 Size: 29 26 2036 496 2556 Fragments arranged by size: 2556 2036 496 29 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2593 3354 4382 1540 Size: 275 186 78 514 761 1028 2301 Fragments arranged by size: 2301 1028 761 514 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2161 3204 3289 4360 2161 Size: 1043 85 1071 2944 Fragments arranged by size: 2944 1071 1043 85 BsmI GAATG_Cn' Cuts at: 2366 2654 2747 2366 Size: 288 93 4762 Fragments arranged by size: 4762 288 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2072 2161 2408 2462 3204 3289 Size: 399 226 56 89 247 54 742 85 Cuts at: 3289 4360 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 247 226 89 85 56 54 BspEI T'CCGG_A Cuts at: 4806 4806 Size: 5143 BspHI T'CATG_A Cuts at: 3032 4762 3032 Size: 1730 3413 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2498 844 Size: 1371 283 3489 Fragments arranged by size: 3489 1371 283 BsrBI CCG'CTC Cuts at: 1394 1823 3030 1394 Size: 429 1207 3507 Fragments arranged by size: 3507 1207 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3638 3812 65 Size: 1183 2390 174 1396 Fragments arranged by size: 2390 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2078 3787 3974 1700 Size: 110 268 1709 187 2869 Fragments arranged by size: 2869 1709 268 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5143 BssHII G'CGCG_C Cuts at: 1925 2020 2217 2233 1925 Size: 95 197 16 4835 Fragments arranged by size: 4835 197 95 16 BssSI C'ACGA_G Cuts at: 3203 4215 3203 Size: 1012 4131 BstAPI GCAn_nnn'nTGC Cuts at: 794 2230 794 Size: 1436 3707 BstBI TT'CG_AA Cuts at: 3947 3947 Size: 5143 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5143 BstYI r'GATC_y Cuts at: 1972 2382 2473 2840 3225 3242 4694 4780 4792 Size: 410 91 367 385 17 1452 86 12 Cuts at: 4792 5138 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 410 385 367 346 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2052 2073 2121 513 Size: 552 939 48 21 48 3535 Fragments arranged by size: 3535 939 552 48 48 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4404 Fragments arranged by size: 4404 683 56 BtsI GCAGTG_nn' Cuts at: 2659 3453 3473 2659 Size: 794 20 4329 Fragments arranged by size: 4329 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5143 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5108 DraI TTT'AAA Cuts at: 2447 2447 Size: 5143 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2171 4150 809 Size: 507 855 1979 1802 Fragments arranged by size: 1979 1802 855 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2040 2049 Size: 54 1425 50 275 18 177 33 9 Cuts at: 2049 2061 2076 2590 3479 5108 8 Size: 12 15 514 889 1629 43 Fragments arranged by size: 1629 1425 889 514 275 177 54 50 43 33 18 15 12 9 EagI C'GGCC_G Cuts at: 1537 1812 2076 2590 1537 Size: 275 264 514 4090 Fragments arranged by size: 4090 514 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 3073 1353 Size: 1720 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3730 4104 4250 387 Size: 1064 138 2141 374 146 1280 Fragments arranged by size: 2141 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 3206 3803 4590 4858 1239 Size: 393 436 1138 597 787 268 1524 Fragments arranged by size: 1524 1138 787 597 436 393 268 EcoO109I rG'GnC_Cy Cuts at: 2013 2117 2013 Size: 104 5039 EcoRI G'AATT_C Cuts at: 2452 2830 2452 Size: 378 4765 FspI TGC'GCA Cuts at: 3649 3649 Size: 5143 HaeII r_GCGC'y Cuts at: 1678 4290 1678 Size: 2612 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3784 3816 3858 3890 1373 Size: 32 135 32 2212 32 42 32 2626 Fragments arranged by size: 2626 2212 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2487 2733 669 Size: 988 281 549 246 3079 Fragments arranged by size: 3079 988 549 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5143 HpaI GTT'AAC Cuts at: 2733 2733 Size: 5143 KpnI G_GTAC'C Cuts at: 2468 2468 Size: 5143 MfeI C'AATT_G Cuts at: 2742 2742 Size: 5143 MluI A'CGCG_T Cuts at: 5019 5019 Size: 5143 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4257 4441 1305 Size: 2952 184 2007 Fragments arranged by size: 2952 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 10 Size: 54 1425 343 210 3111 Fragments arranged by size: 3111 1425 343 210 54 MslI CAynn'nnrTG Cuts at: 518 1166 3101 3460 3619 518 Size: 648 1935 359 159 2042 Fragments arranged by size: 2042 1935 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2029 2075 2559 3236 4384 4629 1606 Size: 423 46 484 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 484 423 245 46 NaeI GCC'GGC Cuts at: 2080 2080 Size: 5143 NcoI C'CATG_G Cuts at: 513 1065 2004 2121 513 Size: 552 939 117 3535 Fragments arranged by size: 3535 939 552 117 NdeI CA'TA_TG Cuts at: 387 387 Size: 5143 NgoMIV G'CCGG_C Cuts at: 2078 2078 Size: 5143 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5143 NotI GC'GGCC_GC Cuts at: 2590 2590 Size: 5143 NspI r_CATG'y Cuts at: 1132 1229 2206 2501 4046 1132 Size: 97 977 295 1545 2229 Fragments arranged by size: 2229 1545 977 295 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5143 PciI A'CATG_T Cuts at: 2202 4042 2202 Size: 1840 3303 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5143 PmeI GTTT'AAAC Cuts at: 2447 2447 Size: 5143 PmlI CAC'GTG Cuts at: 2298 2298 Size: 5143 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3212 3244 4725 4757 3212 Size: 32 1481 32 3598 Fragments arranged by size: 3598 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5143 PsiI TTA'TAA Cuts at: 2713 2713 Size: 5143 PspOMI G'GGCC_C Cuts at: 2012 2012 Size: 5143 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2284 2316 2284 Size: 32 5111 PstI C_TGCA'G Cuts at: 830 2226 2495 830 Size: 1396 269 3478 Fragments arranged by size: 3478 1396 269 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5143 SacII CC_GC'GG Cuts at: 2076 2076 Size: 5143 SalI G'TCGA_C Cuts at: 1936 2485 1936 Size: 549 4594 SbfI CC_TGCA'GG Cuts at: 2495 2495 Size: 5143 ScaI AGT'ACT Cuts at: 3391 5103 3391 Size: 1712 3431 SfcI C'TryA_G Cuts at: 826 1045 2222 2491 3626 4307 4498 826 Size: 219 1177 269 1135 681 191 1471 Fragments arranged by size: 1471 1177 1135 681 269 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2048 2048 Size: 5143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5143 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5143 SmaI CCC'GGG Cuts at: 1872 2470 1872 Size: 598 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3247 4148 4410 820 Size: 197 926 1304 901 262 1553 Fragments arranged by size: 1553 1304 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2934 493 Size: 2441 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5143 SphI G_CATG'C Cuts at: 1132 2501 1132 Size: 1369 3774 SspI AAT'ATT Cuts at: 5 52 3067 5 Size: 47 3015 2081 Fragments arranged by size: 3015 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5143 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2396 2582 513 Size: 552 939 117 275 186 3074 Fragments arranged by size: 3074 939 552 275 186 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3170 3187 3340 3525 3970 325 Size: 1255 1590 17 153 185 445 1498 Fragments arranged by size: 1590 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 3389 5101 Size: 274 80 33 51 1443 204 1208 1712 Cuts at: 5101 96 Size: 138 Fragments arranged by size: 1712 1443 1208 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5103 5135 5103 Size: 32 5111 Tth111I GACn'n_nGTC Cuts at: 1316 2416 1316 Size: 1100 4043 XbaI T'CTAG_A Cuts at: 2479 2479 Size: 5143 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2050 Size: 5143 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5143 XmaI C'CCGG_G Cuts at: 1870 2468 1870 Size: 598 4545 XmnI GAAnn'nnTTC Cuts at: 2267 2366 3272 2267 Size: 99 906 4138 Fragments arranged by size: 4138 906 99 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2951 276 Size: 53 83 186 936 1417 2468 Fragments arranged by size: 2468 1417 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspXI PvuII RsrII SacI SanDI SapI SexAI SfoI SrfI SwaI