################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrGI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HgaI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 666 GTCTAC GT^[AC][GT]AC AcyI 564 GGCGCC G[GA]^CG[CT]C AfaI 902 GTAC GT^AC Alw44I 844 GTGCAC G^TGCAC ApaI 1321 GGGCCC GGGCC^C ApaLI 844 GTGCAC G^TGCAC BamHI 169 GGATCC G^GATCC BbeI 564 GGCGCC GGCGC^C BglI 58 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 564 GGCGCC G[GA]^CG[CT]C BsgI 323 GTGCAG GTGCAG(16/14) BsiEI 117 CGGCCG CG[GA][CT]^CG BsmAI 889 GTCTC GTCTC(1/5) BspHI 608 TCATGA T^CATGA BsrFI 119 GCCGGC [GA]^CCGG[CT] BssHII 96 GCGCGC G^CGCGC BssSI 735 CTCGTG CACGAG(-5/-1) BstBI 817 TTCGAA TT^CGAA BstYI 169 GGATCC [GA]^GATC[CT] Cfr10I 119 GCCGGC [GA]^CCGG[CT] DrdI 625 GACACCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 117 CGGCCG C^GGCCG EclXI 117 CGGCCG C^GGCCG Eco52I 117 CGGCCG C^GGCCG FseI 118 GGCCGGCC GGCCGG^CC HaeII 564 GGCGCC [GA]GCGC^[CT] Hin1I 564 GGCGCC G[GA]^CG[CT]C KasI 564 GGCGCC G^GCGCC KspI 836 CCGCGG CCGC^GG MaeII 398 ACGT A^CGT MflI 169 GGATCC [GA]^GATC[CT] NaeI 119 GCCGGC GCC^GGC NarI 564 GGCGCC GG^CGCC NgoMIV 119 GCCGGC G^CCGGC NspV 817 TTCGAA TT^CGAA PpuMI 947 AGGACCC [GA]G^G[AT]CC[CT] PvuII 1291 CAGCTG CAG^CTG RcaI 608 TCATGA T^CATGA RsaI 902 GTAC GT^AC SacII 836 CCGCGG CCGC^GG SapI 258 GAAGAGC GCTCTTC(1/4) SfoI 564 GGCGCC GGC^GCC SfuI 817 TTCGAA TT^CGAA TfiI 198 GATTC G^A[AT]TC XhoII 169 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwI 170 GATCC GGATC(4/5) 169 GGATC AlwNI 1276 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1288 CAGCAGCTG BanI 564 GGCGCC G^G[CT][GA]CC 991 GGTGCC BbsI 963 GAAGAC GAAGAC(2/6) 487 GTCTTC BpuAI 963 GAAGAC GAAGAC(2/6) 487 GTCTTC BsrBI 114 GAGCGG CCGCTC(-3/-3) 1105 GAGCGG BsrDI 585 CATTGC GCAATG(2/0) 299 GCAATG DpnII 170 GATC ^GATC 331 GATC DraII 947 AGGACCC [GA]G^G[ACGT]CC[CT] 1320 AGGGCCC EarI 258 GAAGAG CTCTTC(1/4) 979 GAAGAG EcoO109I 947 AGGACCC [GA]G^G[ACGT]CC[CT] 1320 AGGGCCC HphI 230 TCACC GGTGA(8/7) 746 TCACC Ksp632I 258 GAAGAG CTCTTC(1/4) 979 GAAGAG MaeIII 623 GTGAC ^GT[ACGT]AC 1309 GTGAC MboI 170 GATC ^GATC 331 GATC MslI 417 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 921 CACAGATGTG NcoI 1113 CCATGG C^CATGG 1220 CCATGG NdeII 170 GATC ^GATC 331 GATC PflMI 798 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1029 CCAGGTCTTGG PleI 868 GACTC GAGTC(4/5) 1311 GACTC SacI 4 GAGCTC GAGCT^C 22 GAGCTC Sau3AI 170 GATC ^GATC 331 GATC SfaNI 1233 GATGC GCATC(5/9) 344 GCATC TaqI 575 TCGA T^CGA 818 TCGA Tsp45I 623 GTGAC ^GT[GC]AC 1309 GTGAC TthHB8I 575 TCGA T^CGA 818 TCGA Van91I 798 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1029 CCAGGTCTTGG XcmI 132 CCAAAAAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 395 CCAACGTGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AluI AspHI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PstI Sau96I ScrFI SfcI StyI TseI TspRI