(Circular) (Six-base) MAPSORT of: pFN21AE2350.seq Check: 2480 from: 1 to: 6043 pFN21AE2350 6043 bp With 182 enzymes: SgfI * May 18, 2011 13:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2672 3386 1937 Size: 735 714 4594 Fragments arranged by size: 4594 735 714 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2282 2419 2675 2987 3272 4106 5490 5758 Size: 650 137 256 312 285 834 1384 268 Cuts at: 5758 1632 Size: 1917 Fragments arranged by size: 1917 1384 834 650 312 285 268 256 137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 2426 2930 1166 Size: 1260 504 4279 Fragments arranged by size: 4279 1260 504 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2874 1184 Size: 1690 4353 AlwNI CAG_nnn'CTG Cuts at: 3286 3298 3786 5358 3286 Size: 12 488 1572 3971 Fragments arranged by size: 3971 1572 488 12 ApaI G_GGCC'C Cuts at: 3330 3330 Size: 6043 ApaLI G'TGCA_C Cuts at: 2849 4100 5256 2849 Size: 1251 1156 3636 Fragments arranged by size: 3636 1251 1156 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 3368 1870 Size: 73 1425 4545 Fragments arranged by size: 4545 1425 73 BamHI G'GATC_C Cuts at: 2174 3373 3740 2174 Size: 1199 367 4477 Fragments arranged by size: 4477 1199 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2569 2996 3364 4819 618 Size: 325 217 627 782 427 368 1455 1842 Fragments arranged by size: 1842 1455 782 627 427 368 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2031 3330 3362 1391 Size: 622 18 1299 32 4072 Fragments arranged by size: 4072 1299 622 32 18 BbeI G_GCGC'C Cuts at: 2573 2573 Size: 6043 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2485 2975 928 Size: 971 586 490 3996 Fragments arranged by size: 3996 971 586 490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4234 4268 1903 Size: 34 2297 34 3678 Fragments arranged by size: 3678 2297 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 4654 136 Size: 107 122 71 631 605 397 2585 1525 Fragments arranged by size: 2585 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 6038 6038 Size: 6043 BlpI GC'TnA_GC Cuts at: 3460 3460 Size: 6043 Bme1580I G_kGCm'C Cuts at: 1790 2853 3330 4104 5260 1790 Size: 1063 477 774 1156 2573 Fragments arranged by size: 2573 1156 1063 774 477 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3774 4732 442 Size: 416 53 83 399 24 2357 958 1753 Fragments arranged by size: 2357 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2374 2608 2668 4703 1239 Size: 1135 234 60 2035 2579 Fragments arranged by size: 2579 2035 1135 234 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4168 5033 5331 4168 Size: 865 298 4880 Fragments arranged by size: 4880 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4706 882 Size: 617 3207 2219 Fragments arranged by size: 3207 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3834 493 Size: 711 2630 2702 Fragments arranged by size: 2702 2630 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2570 3850 4232 4867 Size: 53 83 186 936 1037 1280 382 635 Cuts at: 4867 275 Size: 1451 Fragments arranged by size: 1451 1280 1037 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4476 4874 5148 5295 5706 4476 Size: 398 274 147 411 4813 Fragments arranged by size: 4813 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5818 Fragments arranged by size: 5818 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2131 3113 3248 1186 Size: 940 5 982 135 3981 Fragments arranged by size: 3981 982 940 135 5 BseYI C'CCAG_C Cuts at: 5246 5742 5246 Size: 496 5547 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 1163 Size: 573 613 4857 Fragments arranged by size: 4857 613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2125 3493 4254 5282 1540 Size: 275 186 124 1368 761 1028 2301 Fragments arranged by size: 2301 1368 1028 761 275 186 124 BsiHKAI G_wGCw'C Cuts at: 2013 2031 2360 2385 2853 3275 4104 4189 5260 Size: 18 329 25 468 422 829 85 1071 Cuts at: 5260 2013 Size: 2796 Fragments arranged by size: 2796 1071 829 468 422 329 85 25 18 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2031 2360 2385 2853 3275 3330 Size: 399 223 18 329 25 468 422 55 Cuts at: 3330 3362 4104 4189 5260 1391 Size: 32 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 468 422 399 329 223 85 55 32 25 18 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 2613 3932 5662 2613 Size: 1319 1730 2994 Fragments arranged by size: 2994 1730 1319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3398 844 Size: 2554 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2121 3112 3930 1394 Size: 429 298 991 818 3507 Fragments arranged by size: 3507 991 818 429 298 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 2589 4538 4712 65 Size: 1183 1063 278 1949 174 1396 Fragments arranged by size: 1949 1396 1183 1063 278 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2124 4687 4874 1700 Size: 110 314 2563 187 2869 Fragments arranged by size: 2869 2563 314 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 2101 1925 Size: 176 5867 BssSI C'ACGA_G Cuts at: 2740 4103 5115 2740 Size: 1363 1012 3668 Fragments arranged by size: 3668 1363 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 2823 4847 2823 Size: 2024 4019 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2174 3373 3740 4125 4142 5594 5680 5692 Size: 202 1199 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1199 385 367 346 202 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2841 3118 3225 513 Size: 552 939 837 277 107 3331 Fragments arranged by size: 3331 939 837 552 277 107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 3559 4353 4373 3559 Size: 794 20 5229 Fragments arranged by size: 5229 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2636 5050 809 Size: 507 1320 2414 1802 Fragments arranged by size: 2414 1802 1320 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2122 2469 3048 Size: 54 1425 50 275 18 292 347 579 Cuts at: 3048 3157 3214 3490 4379 6008 8 Size: 109 57 276 889 1629 43 Fragments arranged by size: 1629 1425 889 579 347 292 276 275 109 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2122 3490 1537 Size: 275 310 1368 4090 Fragments arranged by size: 4090 1368 310 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2258 2979 3973 1353 Size: 905 721 994 3423 Fragments arranged by size: 3423 994 905 721 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4630 5004 5150 387 Size: 1064 138 3041 374 146 1280 Fragments arranged by size: 3041 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2029 2011 Size: 18 6025 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2282 2374 2419 2608 2668 2675 2987 Size: 393 650 92 45 189 60 7 312 Cuts at: 2987 3272 4106 4703 5490 5758 1239 Size: 285 834 597 787 268 1524 Fragments arranged by size: 1524 834 787 650 597 393 312 285 268 189 92 60 45 7 EcoO109I rG'GnC_Cy Cuts at: 2953 3326 2953 Size: 373 5670 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FseI GG_CCGG'CC Cuts at: 2128 2128 Size: 6043 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 2573 5190 1678 Size: 895 2617 2531 Fragments arranged by size: 2617 2531 895 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2442 2474 4684 4716 4758 Size: 32 135 32 870 32 2210 32 42 Cuts at: 4758 4790 1373 Size: 32 2626 Fragments arranged by size: 2626 2210 870 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KasI G'GCGC_C Cuts at: 2569 2569 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 2892 3288 5157 5341 1305 Size: 699 888 396 1869 184 2007 Fragments arranged by size: 2007 1869 888 699 396 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2471 3050 3216 10 Size: 54 1425 343 639 579 166 2837 Fragments arranged by size: 2837 1425 639 579 343 166 54 MslI CAynn'nnrTG Cuts at: 518 1166 2426 2930 4001 4360 4519 518 Size: 648 1260 504 1071 359 159 2042 Fragments arranged by size: 2042 1260 1071 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2200 2274 2843 3298 3459 4136 5284 5529 Size: 594 74 569 455 161 677 1148 245 Cuts at: 5529 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 594 569 455 245 161 74 NaeI GCC'GGC Cuts at: 2126 2126 Size: 6043 NarI GG'CG_CC Cuts at: 2570 2570 Size: 6043 NcoI C'CATG_G Cuts at: 513 1065 2004 3118 3225 513 Size: 552 939 1114 107 3331 Fragments arranged by size: 3331 1114 939 552 107 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NgoMIV G'CCGG_C Cuts at: 2124 2124 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3401 4946 1132 Size: 97 2172 1545 2229 Fragments arranged by size: 2229 2172 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6043 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2809 3040 1108 Size: 1701 231 4111 Fragments arranged by size: 4111 1701 231 PfoI T'CCnGG_A Cuts at: 2802 2802 Size: 6043 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2953 2953 Size: 6043 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PspOMI G'GGCC_C Cuts at: 3326 3326 Size: 6043 PstI C_TGCA'G Cuts at: 830 2257 2376 2450 3291 3395 830 Size: 1427 119 74 841 104 3478 Fragments arranged by size: 3478 1427 841 119 104 74 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 3298 3298 Size: 6043 SacI G_AGCT'C Cuts at: 2013 2031 2013 Size: 18 6025 SacII CC_GC'GG Cuts at: 2844 2844 Size: 6043 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SapI GCTCTTCn'nnn_ Cuts at: 2258 2258 Size: 6043 SbfI CC_TGCA'GG Cuts at: 3395 3395 Size: 6043 ScaI AGT'ACT Cuts at: 4291 6003 4291 Size: 1712 4331 SfcI C'TryA_G Cuts at: 826 1045 2253 2372 2446 3287 3391 4526 5207 Size: 219 1208 119 74 841 104 1135 681 Cuts at: 5207 5398 826 Size: 191 1471 Fragments arranged by size: 1471 1208 1135 841 681 219 191 119 104 74 SfoI GGC'GCC Cuts at: 2571 2571 Size: 6043 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3370 1872 Size: 1498 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4147 5048 5310 820 Size: 197 926 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6043 StyI C'CwwG_G Cuts at: 513 1065 2004 2361 2898 3045 3118 3225 3482 Size: 552 939 357 537 147 73 107 257 Cuts at: 3482 513 Size: 3074 Fragments arranged by size: 3074 939 552 537 357 257 147 107 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2252 4070 4087 4240 4425 4870 325 Size: 1255 672 1818 17 153 185 445 1498 Fragments arranged by size: 1818 1498 1255 672 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4289 6001 96 Size: 274 80 33 51 1443 2312 1712 138 Fragments arranged by size: 2312 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6003 6035 6003 Size: 32 6011 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2144 2407 2144 Size: 263 5780 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3368 1870 Size: 1498 4545 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SfiI SrfI SwaI