(Linear) (Six-base) MAPSORT of: KIEE2350.seq Check: 5371 from: 1 to: 1338 KIEE2350.orf With 182 enzymes: SgfI * May 18, 2011 13:28 .. AccI GT'mk_AC Cuts at: 0 667 1338 Size: 667 671 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 414 670 982 1267 1338 Size: 277 137 256 312 285 71 Fragments arranged by size: 312 285 277 256 137 71 AleI CACnn'nnGTG Cuts at: 0 421 925 1338 Size: 421 504 413 Fragments arranged by size: 504 421 413 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 869 1338 Size: 869 469 AlwNI CAG_nnn'CTG Cuts at: 0 1281 1293 1338 Size: 1281 12 45 Fragments arranged by size: 1281 45 12 ApaI G_GGCC'C Cuts at: 0 1325 1338 Size: 1325 13 ApaLI G'TGCA_C Cuts at: 0 844 1338 Size: 844 494 BamHI G'GATC_C Cuts at: 0 169 1338 Size: 169 1169 BanI G'GyrC_C Cuts at: 0 564 991 1338 Size: 564 427 347 Fragments arranged by size: 564 427 347 BanII G_rGCy'C Cuts at: 0 8 26 1325 1338 Size: 8 18 1299 13 Fragments arranged by size: 1299 18 13 8 BbeI G_GCGC'C Cuts at: 0 568 1338 Size: 568 770 BbsI GAAGACnn'nnnn_ Cuts at: 0 480 970 1338 Size: 480 490 368 Fragments arranged by size: 490 480 368 BglI GCCn_nnn'nGGC Cuts at: 0 64 1338 Size: 64 1274 Bme1580I G_kGCm'C Cuts at: 0 848 1325 1338 Size: 848 477 13 Fragments arranged by size: 848 477 13 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 369 603 663 1338 Size: 369 234 60 675 Fragments arranged by size: 675 369 234 60 BsaHI Gr'CG_yC Cuts at: 0 565 1338 Size: 565 773 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 121 126 1108 1243 1338 Size: 121 5 982 135 95 Fragments arranged by size: 982 135 121 95 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1338 Size: 344 994 BsiEI CG_ry'CG Cuts at: 0 120 1338 Size: 120 1218 BsiHKAI G_wGCw'C Cuts at: 0 8 26 355 380 848 1270 1338 Size: 8 18 329 25 468 422 68 Fragments arranged by size: 468 422 329 68 25 18 8 Bsp1286I G_dGCh'C Cuts at: 0 8 26 355 380 848 1270 1325 1338 Size: 8 18 329 25 468 422 55 13 Fragments arranged by size: 468 422 329 55 25 18 13 8 BspHI T'CATG_A Cuts at: 0 608 1338 Size: 608 730 BsrBI CCG'CTC Cuts at: 0 116 1107 1338 Size: 116 991 231 Fragments arranged by size: 991 231 116 BsrDI GCAATG_nn' Cuts at: 0 306 584 1338 Size: 306 278 754 Fragments arranged by size: 754 306 278 BsrFI r'CCGG_y Cuts at: 0 119 1338 Size: 119 1219 BssHII G'CGCG_C Cuts at: 0 96 1338 Size: 96 1242 BssSI C'ACGA_G Cuts at: 0 735 1338 Size: 735 603 BstBI TT'CG_AA Cuts at: 0 818 1338 Size: 818 520 BstYI r'GATC_y Cuts at: 0 169 1338 Size: 169 1169 BtgI C'CryG_G Cuts at: 0 836 1113 1220 1338 Size: 836 277 107 118 Fragments arranged by size: 836 277 118 107 DrdI GACnn_nn'nnGTC Cuts at: 0 631 1338 Size: 631 707 EaeI y'GGCC_r Cuts at: 0 117 464 1043 1152 1209 1338 Size: 117 347 579 109 57 129 Fragments arranged by size: 579 347 129 117 109 57 EagI C'GGCC_G Cuts at: 0 117 1338 Size: 117 1221 EarI CTCTTCn'nnn_ Cuts at: 0 253 974 1338 Size: 253 721 364 Fragments arranged by size: 721 364 253 EcoICRI GAG'CTC Cuts at: 0 6 24 1338 Size: 6 18 1314 Fragments arranged by size: 1314 18 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 369 414 603 663 670 982 1267 Size: 277 92 45 189 60 7 312 285 Cuts at: 1267 1338 Size: 71 Fragments arranged by size: 312 285 277 189 92 71 60 45 7 EcoO109I rG'GnC_Cy Cuts at: 0 948 1321 1338 Size: 948 373 17 Fragments arranged by size: 948 373 17 FseI GG_CCGG'CC Cuts at: 0 123 1338 Size: 123 1215 HaeII r_GCGC'y Cuts at: 0 568 1338 Size: 568 770 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 437 469 1338 Size: 437 32 869 Fragments arranged by size: 869 437 32 KasI G'GCGC_C Cuts at: 0 564 1338 Size: 564 774 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 887 1283 1338 Size: 887 396 55 Fragments arranged by size: 887 396 55 MscI TGG'CCA Cuts at: 0 466 1045 1211 1338 Size: 466 579 166 127 Fragments arranged by size: 579 466 166 127 MslI CAynn'nnrTG Cuts at: 0 421 925 1338 Size: 421 504 413 Fragments arranged by size: 504 421 413 MspA1I CmG'CkG Cuts at: 0 195 269 838 1293 1338 Size: 195 74 569 455 45 Fragments arranged by size: 569 455 195 74 45 NaeI GCC'GGC Cuts at: 0 121 1338 Size: 121 1217 NarI GG'CG_CC Cuts at: 0 565 1338 Size: 565 773 NcoI C'CATG_G Cuts at: 0 1113 1220 1338 Size: 1113 107 118 Fragments arranged by size: 1113 118 107 NgoMIV G'CCGG_C Cuts at: 0 119 1338 Size: 119 1219 PflMI CCAn_nnn'nTGG Cuts at: 0 804 1035 1338 Size: 804 231 303 Fragments arranged by size: 804 303 231 PfoI T'CCnGG_A Cuts at: 0 797 1338 Size: 797 541 PpuMI rG'GwC_Cy Cuts at: 0 948 1338 Size: 948 390 PspOMI G'GGCC_C Cuts at: 0 1321 1338 Size: 1321 17 PstI C_TGCA'G Cuts at: 0 252 371 445 1286 1338 Size: 252 119 74 841 52 Fragments arranged by size: 841 252 119 74 52 PvuII CAG'CTG Cuts at: 0 1293 1338 Size: 1293 45 SacI G_AGCT'C Cuts at: 0 8 26 1338 Size: 8 18 1312 Fragments arranged by size: 1312 18 8 SacII CC_GC'GG Cuts at: 0 839 1338 Size: 839 499 SapI GCTCTTCn'nnn_ Cuts at: 0 253 1338 Size: 253 1085 SfcI C'TryA_G Cuts at: 0 248 367 441 1282 1338 Size: 248 119 74 841 56 Fragments arranged by size: 841 248 119 74 56 SfoI GGC'GCC Cuts at: 0 566 1338 Size: 566 772 StyI C'CwwG_G Cuts at: 0 356 893 1040 1113 1220 1338 Size: 356 537 147 73 107 118 Fragments arranged by size: 537 356 147 118 107 73 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 247 1338 Size: 247 1091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 139 402 1338 Size: 139 263 936 Fragments arranged by size: 936 263 139 Enzymes that do cut: AccI AcuI AleI AlfI AlwNI ApaI ApaLI BamHI BanI BanII BbeI BbsI BglI Bme1580I BpmI BsaHI BseRI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstYI BtgI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoO109I FseI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI StyI TaqII XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI BsiWI BsmI BsmBI BspEI BspMI BsrGI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EciI EcoNI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI