Restriction map for
pFN21AB5919
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaHI BsaI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Cac8I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MfeI MluI
MroI MslI MspA1I MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tth111I TthHB8I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 994 GTAGAC GT^[AC][GT]AC
AccII 533 CGCG CG^CG
AfaI 30 GTAC GT^AC
AgeI 409 ACCGGT A^CCGGT
AlwNI 703 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 98 GGGCCC GGGCC^C
BalI 129 TGGCCA TGG^CCA
BamHI 807 GGATCC G^GATCC
BanI 1315 GGTGCC G^G[CT][GA]CC
BanII 98 GGGCCC G[GA]GC[CT]^C
BglII 1059 AGATCT A^GATCT
BsaAI 310 TACGTG [CT]AC^GT[GA]
BsaBI 716 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 409 ACCGGT [AT]^CCGG[AT]
BsmFI 637 GTCCC GGGAC(10/14)
BsmI 1410 GAATGC GAATGC(1/-1)
BspMI 193 GCAGGT ACCTGC(4/8)
BstAPI 1006 GCACAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 533 CGCG CG^CG
BstXI 245 CCAGACACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1201 GCGC GCG^C
DrdI 929 GACATGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 129 TGGCCA [CT]^GGCC[GA]
EarI 1482 CTCTTC CTCTTC(1/4)
EcoNI 822 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 451 GAATTC G^AATTC
HhaI 1201 GCGC GCG^C
HinP1I 1201 GCGC G^CGC
HincII 75 GTCAAC GT[CT]^[GA]AC
HindII 75 GTCAAC GT[CT]^[GA]AC
HindIII 862 AAGCTT A^AGCTT
Ksp632I 1482 CTCTTC CTCTTC(1/4)
MaeII 311 ACGT A^CGT
MamI 716 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 129 TGGCCA TGG^CCA
MscI 129 TGGCCA TGG^CCA
MvnI 533 CGCG CG^CG
PflMI 1329 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 409 ACCGGT A^CCGGT
PpuMI 635 AGGTCCC [GA]G^G[AT]CC[CT]
RsaI 30 GTAC GT^AC
SexAI 795 ACCAGGT A^CC[AT]GGT
SmlI 180 CTTGAG C^T[CT][GA]AG
Van91I 1329 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1165 TCTAGA T^CTAGA
XcmI 954 CCAAAATGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AocI 822 CCTGAGG CC^T[ACGT]AGG
1420 CCTAAGG
Asp700I 542 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1207 GAAGCCATTC
AvaII 636 GGTCC G^G[AT]CC
672 GGACC
BbsI 1150 GAAGAC GAAGAC(2/6)
1462 GAAGAC
BbvCI 172 GCTGAGG CCTCAGC(-5/-2)
316 GCTGAGG
BbvI 1066 GCTGC GCAGC(8/12)
1182 GCTGC
BfaI 34 CTAG C^TAG
1166 CTAG
BmyI 98 GGGCCC G[AGT]GC[ACT]^C
1316 GTGCCC
BpuAI 1150 GAAGAC GAAGAC(2/6)
1462 GAAGAC
Bsp1286I 98 GGGCCC G[AGT]GC[ACT]^C
1316 GTGCCC
BsrFI 409 ACCGGT [GA]^CCGG[CT]
1185 GCCGGT
Bsu36I 822 CCTGAGG CC^T[ACGT]AGG
1420 CCTAAGG
Cfr10I 409 ACCGGT [GA]^CCGG[CT]
1185 GCCGGT
Eco81I 822 CCTGAGG CC^T[ACGT]AGG
1420 CCTAAGG
HapII 410 CCGG C^CGG
1186 CCGG
HpaII 410 CCGG C^CGG
1186 CCGG
MaeI 34 CTAG C^TAG
1166 CTAG
MspI 410 CCGG C^CGG
1186 CCGG
SfaNI 373 GATGC GCATC(5/9)
1495 GATGC
TseI 1066 GCTGC G^C[AT]GC
1182 GCTGC
XmnI 542 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1207 GAAGCCATTC
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BpmI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraII DsaI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfcI TfiI
Tru9I Tsp45I Tsp509I TspRI XhoII