(Circular) (Six-base) MAPSORT of: pFN21AB5919.seq Check: 9658 from: 1 to: 6220 pFN21AB5919 6220 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2189 2189 Size: 6220 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3000 3563 1937 Size: 1063 563 4594 Fragments arranged by size: 4594 1063 563 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2735 4283 5667 5935 1632 Size: 1103 1548 1384 268 1917 Fragments arranged by size: 1917 1548 1384 1103 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 5119 6096 1203 Size: 3916 977 1327 Fragments arranged by size: 3916 1327 977 AgeI A'CCGG_T Cuts at: 2414 5051 2414 Size: 2637 3583 AhdI GACnn_n'nnGTC Cuts at: 4949 4949 Size: 6220 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2034 2066 2034 Size: 32 6188 AlwNI CAG_nnn'CTG Cuts at: 2713 3963 5535 2713 Size: 1250 1572 3398 Fragments arranged by size: 3398 1572 1250 ApaI G_GGCC'C Cuts at: 2107 2107 Size: 6220 ApaLI G'TGCA_C Cuts at: 4277 5433 4277 Size: 1156 5064 ApoI r'AATT_y Cuts at: 1493 2456 2567 3230 3529 3755 3907 6016 1493 Size: 963 111 663 299 226 152 2109 1697 Fragments arranged by size: 2109 1697 963 663 299 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 4774 160 Size: 872 3742 1606 Fragments arranged by size: 3742 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 3545 1870 Size: 73 1602 4545 Fragments arranged by size: 4545 1602 73 BamHI G'GATC_C Cuts at: 2812 3550 3917 2812 Size: 738 367 5115 Fragments arranged by size: 5115 738 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3320 3541 4996 618 Size: 325 217 627 1533 221 1455 1842 Fragments arranged by size: 1842 1533 1455 627 325 221 217 BanII G_rGCy'C Cuts at: 1391 2107 3539 1391 Size: 716 1432 4072 Fragments arranged by size: 4072 1432 716 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3162 3474 928 Size: 971 1263 312 3674 Fragments arranged by size: 3674 1263 971 312 BbvCI CC'TCA_GC Cuts at: 2178 2322 2178 Size: 144 6076 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4411 4445 1903 Size: 34 2474 34 3678 Fragments arranged by size: 3678 2474 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3064 6215 3064 Size: 3151 3069 BlpI GC'TnA_GC Cuts at: 3637 3637 Size: 6220 Bme1580I G_kGCm'C Cuts at: 1790 2107 3325 4281 5437 1790 Size: 317 1218 956 1156 2573 Fragments arranged by size: 2573 1218 1156 956 317 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2113 3951 4909 Size: 416 53 83 399 24 696 1838 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1838 1753 958 696 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2263 2760 3059 4880 1239 Size: 1024 497 299 1821 2579 Fragments arranged by size: 2579 1821 1024 497 299 Bpu10I CC'TnA_GC Cuts at: 2178 2322 2178 Size: 144 6076 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2206 4345 5210 5508 2206 Size: 2139 865 298 2918 Fragments arranged by size: 2918 2139 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2317 4011 493 Size: 711 1113 1694 2702 Fragments arranged by size: 2702 1694 1113 711 BsaBI GATnn'nnATC Cuts at: 2725 6214 2725 Size: 3489 2731 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2414 4653 5051 5325 5472 5883 2414 Size: 2239 398 274 147 411 2751 Fragments arranged by size: 2751 2239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2034 2064 2472 2502 2684 Size: 30 165 30 631 30 408 30 182 Cuts at: 2684 2714 1178 Size: 30 4684 Fragments arranged by size: 4684 631 408 182 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6220 BseYI C'CCAG_C Cuts at: 2614 2644 5423 5919 2614 Size: 30 2779 496 2915 Fragments arranged by size: 2915 2779 496 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5647 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3670 4431 5459 1540 Size: 275 186 1669 761 1028 2301 Fragments arranged by size: 2301 1669 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4281 4366 5437 4281 Size: 85 1071 5064 Fragments arranged by size: 5064 1071 85 BsmI GAATG_Cn' Cuts at: 3421 3731 3824 3421 Size: 310 93 5817 Fragments arranged by size: 5817 310 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 3325 3539 4281 4366 5437 1391 Size: 399 317 1218 214 742 85 1071 2174 Fragments arranged by size: 2174 1218 1071 742 399 317 214 85 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 4109 5839 4109 Size: 1730 4490 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2189 3575 844 Size: 1345 1386 3489 Fragments arranged by size: 3489 1386 1345 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4715 4889 65 Size: 1183 3467 174 1396 Fragments arranged by size: 3467 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2414 3190 4864 5051 1700 Size: 110 604 776 1674 187 2869 Fragments arranged by size: 2869 1674 776 604 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 4280 5292 4280 Size: 1012 5208 BstAPI GCAn_nnn'nTGC Cuts at: 794 3017 794 Size: 2223 3997 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6220 BstXI CCAn_nnnn'nTGG Cuts at: 2257 2257 Size: 6220 BstYI r'GATC_y Cuts at: 1972 2634 2726 2812 2902 3064 3550 3917 4302 Size: 662 92 86 90 162 486 367 385 Cuts at: 4302 4319 5771 5857 5869 6215 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 662 486 385 367 346 162 92 90 86 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2828 3426 2828 Size: 598 5622 BtgI C'CryG_G Cuts at: 513 1065 2064 2348 2673 513 Size: 552 999 284 325 4060 Fragments arranged by size: 4060 999 552 325 284 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5481 Fragments arranged by size: 5481 683 56 BtsI GCAGTG_nn' Cuts at: 2790 3449 3736 4530 4550 2790 Size: 659 287 794 20 4460 Fragments arranged by size: 4460 794 659 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3524 3524 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2940 5227 809 Size: 507 1624 2287 1802 Fragments arranged by size: 2287 1802 1624 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 3667 4556 Size: 54 1425 50 275 18 304 1533 889 Cuts at: 4556 6185 8 Size: 1629 43 Fragments arranged by size: 1629 1533 1425 889 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3667 1537 Size: 275 1855 4090 Fragments arranged by size: 4090 1855 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3493 4150 1353 Size: 2140 657 3423 Fragments arranged by size: 3423 2140 657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4807 5181 5327 387 Size: 1064 138 3218 374 146 1280 Fragments arranged by size: 3218 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2263 2735 2760 3059 4283 4880 5667 Size: 393 631 472 25 299 1224 597 787 Cuts at: 5667 5935 1239 Size: 268 1524 Fragments arranged by size: 1524 1224 787 631 597 472 393 299 268 25 EcoNI CCTnn'n_nnAGG Cuts at: 2831 2831 Size: 6220 EcoO109I rG'GnC_Cy Cuts at: 2103 2104 2641 2103 Size: 1 537 5682 Fragments arranged by size: 5682 537 1 EcoRI G'AATT_C Cuts at: 2456 3529 3907 2456 Size: 1073 378 4769 Fragments arranged by size: 4769 1073 378 FspI TGC'GCA Cuts at: 4726 4726 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 5367 1678 Size: 3689 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2080 2112 2517 2549 2684 Size: 32 135 32 508 32 405 32 135 Cuts at: 2684 2716 2957 2964 2989 2996 3239 3271 3328 Size: 32 241 7 25 7 243 32 57 Cuts at: 3328 3360 4861 4893 4935 4967 1373 Size: 32 1501 32 42 32 2626 Fragments arranged by size: 2626 1501 508 405 243 241 135 135 57 42 32 32 32 32 32 32 32 32 32 25 7 7 HincII GTy'rAC Cuts at: 669 1657 1938 2082 3564 3810 669 Size: 988 281 144 1482 246 3079 Fragments arranged by size: 3079 1482 988 281 246 144 HindIII A'AGCT_T Cuts at: 748 2867 748 Size: 2119 4101 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 3819 3819 Size: 6220 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3384 3396 3437 5334 5518 1305 Size: 2079 12 41 1897 184 2007 Fragments arranged by size: 2079 2007 1897 184 41 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2136 10 Size: 54 1425 343 304 4094 Fragments arranged by size: 4094 1425 343 304 54 MslI CAynn'nnrTG Cuts at: 518 1166 4178 4537 4696 518 Size: 648 3012 359 159 2042 Fragments arranged by size: 3012 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3636 4313 5461 5706 1606 Size: 2030 677 1148 245 2120 Fragments arranged by size: 2120 2030 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5668 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3578 5123 1132 Size: 97 2349 1545 2229 Fragments arranged by size: 2349 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 3340 1108 Size: 2232 3988 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2034 2066 2336 2368 4289 4321 5802 5834 2034 Size: 32 270 32 1921 32 1481 32 2420 Fragments arranged by size: 2420 1921 1481 270 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 2641 Size: 6220 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2103 2103 Size: 6220 PstI C_TGCA'G Cuts at: 830 3572 830 Size: 2742 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 4468 6180 4468 Size: 1712 4508 SexAI A'CCwGG_T Cuts at: 2800 2800 Size: 6220 SfcI C'TryA_G Cuts at: 826 1045 2336 2497 3352 3568 4703 5384 5575 Size: 219 1291 161 855 216 1135 681 191 Cuts at: 5575 826 Size: 1471 Fragments arranged by size: 1471 1291 1135 855 681 219 216 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 3547 1872 Size: 1675 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2185 4324 5225 5487 820 Size: 197 926 242 2139 901 262 1553 Fragments arranged by size: 2139 1553 926 901 262 242 197 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 4144 5 Size: 47 4092 2081 Fragments arranged by size: 4092 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 3659 513 Size: 552 2594 3074 Fragments arranged by size: 3074 2594 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4247 4264 4417 4602 5047 325 Size: 1255 2667 17 153 185 445 1498 Fragments arranged by size: 2667 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4466 6178 96 Size: 274 80 33 51 1443 2489 1712 138 Fragments arranged by size: 2489 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2744 2776 6180 6212 2744 Size: 32 3404 32 2752 Fragments arranged by size: 3404 2752 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3170 3556 3170 Size: 386 5834 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2966 2966 Size: 6220 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 3545 1870 Size: 1675 4545 XmnI GAAnn'nnTTC Cuts at: 2551 3216 4349 2551 Size: 665 1133 4422 Fragments arranged by size: 4422 1133 665 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI