Restriction map for
pFN21AB5919
(Linear) (Six-base) MAPSORT of: KIBB5919.seq Check: 5391 from: 1 to: 1515
KIBB5919.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 184 1515
Size: 184 1331
AccI GT'mk_AC
Cuts at: 0 995 1515
Size: 995 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 1515
Size: 730 785
AgeI A'CCGG_T
Cuts at: 0 409 1515
Size: 409 1106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 61 1515
Size: 29 32 1454
Fragments arranged by size:
1454 32 29
AlwNI CAG_nnn'CTG
Cuts at: 0 708 1515
Size: 708 807
ApaI G_GGCC'C
Cuts at: 0 102 1515
Size: 102 1413
ApoI r'AATT_y
Cuts at: 0 451 562 1225 1515
Size: 451 111 663 290
Fragments arranged by size:
663 451 290 111
BamHI G'GATC_C
Cuts at: 0 807 1515
Size: 807 708
BanI G'GyrC_C
Cuts at: 0 1315 1515
Size: 1315 200
BanII G_rGCy'C
Cuts at: 0 102 1515
Size: 102 1413
BbsI GAAGACnn'nnnn_
Cuts at: 0 1157 1469 1515
Size: 1157 312 46
Fragments arranged by size:
1157 312 46
BbvCI CC'TCA_GC
Cuts at: 0 173 317 1515
Size: 173 144 1198
Fragments arranged by size:
1198 173 144
BglII A'GATC_T
Cuts at: 0 1059 1515
Size: 1059 456
Bme1580I G_kGCm'C
Cuts at: 0 102 1320 1515
Size: 102 1218 195
Fragments arranged by size:
1218 195 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 108 1515
Size: 108 1407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 755 1054 1515
Size: 258 497 299 461
Fragments arranged by size:
497 461 299 258
Bpu10I CC'TnA_GC
Cuts at: 0 173 317 1515
Size: 173 144 1198
Fragments arranged by size:
1198 173 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 1515
Size: 201 1314
BsaAI yAC'GTr
Cuts at: 0 312 1515
Size: 312 1203
BsaBI GATnn'nnATC
Cuts at: 0 720 1515
Size: 720 795
BsaWI w'CCGG_w
Cuts at: 0 409 1515
Size: 409 1106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 59 467 497 679 709 1515
Size: 29 30 408 30 182 30 806
Fragments arranged by size:
806 408 182 30 30 30 29
BseYI C'CCAG_C
Cuts at: 0 609 639 1515
Size: 609 30 876
Fragments arranged by size:
876 609 30
BsmI GAATG_Cn'
Cuts at: 0 1416 1515
Size: 1416 99
Bsp1286I G_dGCh'C
Cuts at: 0 102 1320 1515
Size: 102 1218 195
Fragments arranged by size:
1218 195 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 184 1515
Size: 184 1331
BsrFI r'CCGG_y
Cuts at: 0 409 1185 1515
Size: 409 776 330
Fragments arranged by size:
776 409 330
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1012 1515
Size: 1012 503
BstXI CCAn_nnnn'nTGG
Cuts at: 0 252 1515
Size: 252 1263
BstYI r'GATC_y
Cuts at: 0 629 721 807 897 1059 1515
Size: 629 92 86 90 162 456
Fragments arranged by size:
629 456 162 92 90 86
Bsu36I CC'TnA_GG
Cuts at: 0 823 1421 1515
Size: 823 598 94
Fragments arranged by size:
823 598 94
BtgI C'CryG_G
Cuts at: 0 59 343 668 1515
Size: 59 284 325 847
Fragments arranged by size:
847 325 284 59
BtsI GCAGTG_nn'
Cuts at: 0 785 1444 1515
Size: 785 659 71
Fragments arranged by size:
785 659 71
DrdI GACnn_nn'nnGTC
Cuts at: 0 935 1515
Size: 935 580
EaeI y'GGCC_r
Cuts at: 0 129 1515
Size: 129 1386
EarI CTCTTCn'nnn_
Cuts at: 0 1488 1515
Size: 1488 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 730 755 1054 1515
Size: 258 472 25 299 461
Fragments arranged by size:
472 461 299 258 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 826 1515
Size: 826 689
EcoO109I rG'GnC_Cy
Cuts at: 0 98 99 636 1515
Size: 98 1 537 879
Fragments arranged by size:
879 537 98 1
EcoRI G'AATT_C
Cuts at: 0 451 1515
Size: 451 1064
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 512 544 679 711 952 959
Size: 75 32 405 32 135 32 241 7
Cuts at: 959 984 991 1234 1266 1323 1355 1515
Size: 25 7 243 32 57 32 160
Fragments arranged by size:
405 243 241 160 135 75 57 32
32 32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 77 1515
Size: 77 1438
HindIII A'AGCT_T
Cuts at: 0 862 1515
Size: 862 653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 1391 1432 1515
Size: 1379 12 41 83
Fragments arranged by size:
1379 83 41 12
MscI TGG'CCA
Cuts at: 0 131 1515
Size: 131 1384
PflMI CCAn_nnn'nTGG
Cuts at: 0 1335 1515
Size: 1335 180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 331 363 1515
Size: 29 32 270 32 1152
Fragments arranged by size:
1152 270 32 32 29
PpuMI rG'GwC_Cy
Cuts at: 0 636 1515
Size: 636 879
PspOMI G'GGCC_C
Cuts at: 0 98 1515
Size: 98 1417
SexAI A'CCwGG_T
Cuts at: 0 795 1515
Size: 795 720
SfcI C'TryA_G
Cuts at: 0 331 492 1347 1515
Size: 331 161 855 168
Fragments arranged by size:
855 331 168 161
SmlI C'TyrA_G
Cuts at: 0 180 1515
Size: 180 1335
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 739 771 1515
Size: 739 32 744
Fragments arranged by size:
744 739 32
XbaI T'CTAG_A
Cuts at: 0 1165 1515
Size: 1165 350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 961 1515
Size: 961 554
XmnI GAAnn'nnTTC
Cuts at: 0 546 1211 1515
Size: 546 665 304
Fragments arranged by size:
665 546 304
Enzymes that do cut:
AarI AccI AcuI AgeI AloI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BbvCI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseYI
BsmI Bsp1286I BspMI BsrFI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI Hin4I HincII HindIII MmeI MscI PflMI PpiI
PpuMI PspOMI SexAI SfcI SmlI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BsaI BsaHI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoICRI EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PfoI PmeI
PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI Tth111I
XhoI XmaI ZraI