################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstYI BstZ17I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tru9I Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 439 GAATTC [GA]^AATT[CT] ApoI 439 GAATTC [GA]^AATT[CT] Asp700I 417 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 113 GTGCTC G[AT]GC[AT]^C BanI 100 GGCGCC G^G[CT][GA]CC BbeI 100 GGCGCC GGCGC^C BbsI 327 GAAGAC GAAGAC(2/6) BmyI 113 GTGCTC G[AGT]GC[ACT]^C BpmI 232 CTGGAG CTGGAG(16/14) BpuAI 327 GAAGAC GAAGAC(2/6) BseRI 235 GAGGAG GAGGAG(10/8) BsiHKAI 113 GTGCTC G[AT]GC[AT]^C BsiYI 490 CCACGGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 490 CCACGGAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 260 GAATGC GAATGC(1/-1) Bsp1286I 113 GTGCTC G[AGT]GC[ACT]^C BspMI 398 ACCTGC ACCTGC(4/8) BsrBI 61 CCGCTC CCGCTC(-3/-3) BsrI 231 ACTGG ACTGG(1/-1) BstBI 324 TTCGAA TT^CGAA BstXI 224 CCAATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 47 TGGCCG [CT]^GGCC[GA] EarI 417 GAAGAG CTCTTC(1/4) EcoRI 439 GAATTC G^AATTC HaeII 100 GGCGCC [GA]GCGC^[CT] HapII 38 CCGG C^CGG HgaI 55 GCGTC GACGC(5/10) HpaII 38 CCGG C^CGG HphI 396 TCACC GGTGA(8/7) KasI 100 GGCGCC G^GCGCC Ksp632I 417 GAAGAG CTCTTC(1/4) KspI 50 CCGCGG CCGC^GG MslI 397 CACCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 50 CCGCGG C[AC]G^C[GT]G MspI 38 CCGG C^CGG NarI 100 GGCGCC GG^CGCC NlaIII 403 CATG CATG^ NlaIV 100 GGCGCC GG[ACGT]^[ACGT]CC NspV 324 TTCGAA TT^CGAA PflMI 490 CCACGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 382 CTGCAG CTGCA^G SacII 50 CCGCGG CCGC^GG SfoI 100 GGCGCC GGC^GCC SfuI 324 TTCGAA TT^CGAA SmlI 128 CTCAAG C^T[CT][GA]AG SspI 120 AATATT AAT^ATT Tsp509I 440 AATT ^AATT TspRI 230 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 490 CCACGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 417 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 51 CGCG CG^CG 96 CGCG AcyI 54 GGCGTC G[GA]^CG[CT]C 100 GGCGCC AocI 214 CCTCAGG CC^T[ACGT]AGG 274 CCTCAGG BsaHI 54 GGCGTC G[GA]^CG[CT]C 100 GGCGCC BsmFI 57 GTCCC GGGAC(10/14) 449 GTCCC BssKI 26 CCAGG ^CC[ACGT]GG 89 CCTGG BstAPI 220 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 372 GCATCCGTTGC BstF5I 221 CATCC GGATG(2/0) 373 CATCC BstNI 26 CCAGG CC^[AT]GG 89 CCTGG BstUI 51 CGCG CG^CG 96 CGCG Bsu36I 214 CCTCAGG CC^T[ACGT]AGG 274 CCTCAGG CfoI 95 GCGC GCG^C 101 GCGC DsaI 50 CCGCGG C^C[GA][CT]GG 490 CCACGG Eco81I 214 CCTCAGG CC^T[ACGT]AGG 274 CCTCAGG EcoRII 26 CCAGG ^CC[AT]GG 89 CCTGG FokI 221 CATCC GGATG(9/13) 373 CATCC HaeIII 48 GGCC GG^CC 488 GGCC HhaI 95 GCGC GCG^C 101 GCGC Hin1I 54 GGCGTC G[GA]^CG[CT]C 100 GGCGCC HinP1I 95 GCGC G^CGC 101 GCGC HinfI 205 GATTC G^A[ACGT]TC 422 GATTC MvaI 26 CCAGG CC^[AT]GG 89 CCTGG MvnI 51 CGCG CG^CG 96 CGCG ScrFI 26 CCAGG CC^[ACGT]GG 89 CCTGG SfaNI 220 GCATC GCATC(5/9) 372 GCATC SfcI 154 CTACAG C^T[GA][CT]AG 382 CTGCAG TaqI 325 TCGA T^CGA 468 TCGA TfiI 205 GATTC G^A[AT]TC 422 GATTC TthHB8I 325 TCGA T^CGA 468 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AciI AluI BbvI BsaJI Cac8I DdeI FauI Fnu4HI ItaI MboII MnlI MwoI TseI