################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbsI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI Eam1105I EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 339 GACGCC G[GA]^CG[CT]C AocI 415 CCTGAGG CC^T[ACGT]AGG ApaI 411 GGGCCC GGGCC^C AscI 253 GGCGCGCC GG^CGCGCC AspHI 115 GTGCTC G[AT]GC[AT]^C BanI 348 GGCACC G^G[CT][GA]CC BbrPI 283 CACGTG CAC^GTG BbvCI 475 GCTGAGG CCTCAGC(-5/-2) BcgI 349 GCACCCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 413 GCCCTGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 559 CTGGAG CTGGAG(16/14) BsaAI 283 CACGTG [CT]AC^GT[GA] BsaHI 339 GACGCC G[GA]^CG[CT]C BseRI 451 GAGGAG GAGGAG(10/8) BsgI 193 GTGCAG GTGCAG(16/14) BsiHKAI 115 GTGCTC G[AT]GC[AT]^C BsmBI 61 GAGACG CGTCTC(1/5) BssHII 254 GCGCGC G^CGCGC BstAPI 139 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 144 GGATG GGATG(2/0) BstXI 328 CCAGGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 415 CCTGAGG CC^T[ACGT]AGG DsaI 248 CCGCGG C^C[GA][CT]GG EaeI 19 CGGCCG [CT]^GGCC[GA] EagI 19 CGGCCG C^GGCCG EclXI 19 CGGCCG C^GGCCG Eco52I 19 CGGCCG C^GGCCG Eco81I 415 CCTGAGG CC^T[ACGT]AGG FokI 144 GGATG GGATG(9/13) Hin1I 339 GACGCC G[GA]^CG[CT]C HphI 119 TCACC GGTGA(8/7) KspI 248 CCGCGG CCGC^GG MaeII 284 ACGT A^CGT MboII 616 GAAGA GAAGA(8/7) MspA1I 248 CCGCGG C[AC]G^C[GT]G NspI 367 ACATGC [GA]CATG^[CT] PmaCI 283 CACGTG CAC^GTG PmlI 283 CACGTG CAC^GTG PpuMI 442 GGGACCC [GA]G^G[AT]CC[CT] PstI 67 CTGCAG CTGCA^G SacII 248 CCGCGG CCGC^GG SfcI 67 CTGCAG C^T[GA][CT]AG SmaI 373 CCCGGG CCC^GGG StuI 479 AGGCCT AGG^CCT TfiI 619 GATTC G^A[AT]TC XmaCI 373 CCCGGG C^CCGGG XmaI 373 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AfaI 132 GTAC GT^AC 245 GTAC AlwNI 220 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 392 CAGACCCTG BsaWI 498 ACCGGA [AT]^CCGG[AT] 570 ACCGGA BsiEI 19 CGGCCG CG[GA][CT]^CG 36 CGACCG BsmAI 28 GAGAC GTCTC(1/5) 61 GAGAC BsrBI 16 GAGCGG CCGCTC(-3/-3) 43 GAGCGG DdeI 416 CTGAG C^T[ACGT]AG 476 CTGAG NlaIII 323 CATG CATG^ 368 CATG PleI 76 GACTC GAGTC(4/5) 610 GAGTC RsaI 132 GTAC GT^AC 245 GTAC SfaNI 145 GATGC GCATC(5/9) 157 GATGC TspRI 149 CACTG CA[GC]TG[ACGT][ACGT]^ 334 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccII AciI AluI AvaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TaqI TseI TthHB8I