################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BbrPI BcgI BciVI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsgI BsiEI BsiWI BsmI BspDI BspLU11I BsrBI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MaeI MfeI MluI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI TfiI Tru9I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 1156 GAATTC [GA]^AATT[CT] AocI 870 CCTGAGG CC^T[ACGT]AGG Aor51HI 152 AGCGCT AGC^GCT ApoI 1156 GAATTC [GA]^AATT[CT] AspHI 82 GTGCTC G[AT]GC[AT]^C AvaII 723 GGACC G^G[AT]CC BamHI 866 GGATCC G^GATCC BbvCI 635 CCTCAGC CCTCAGC(-5/-2) BclI 446 TGATCA T^GATCA BsaBI 447 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1207 GAGACC GGTCTC(1/5) BsiHKAI 82 GTGCTC G[AT]GC[AT]^C BsmBI 498 GAGACG CGTCTC(1/5) Bsp1407I 644 TGTACA T^GTACA BspMI 89 ACCTGC ACCTGC(4/8) BsrDI 900 GCAATG GCAATG(2/0) BsrGI 644 TGTACA T^GTACA BssHII 52 GCGCGC G^CGCGC BssSI 133 CACGAG CACGAG(-5/-1) BstXI 1133 CCAGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 866 GGATCC [GA]^GATC[CT] Bsu36I 870 CCTGAGG CC^T[ACGT]AGG DsaI 183 CCGCGG C^C[GA][CT]GG EarI 430 CTCTTC CTCTTC(1/4) Eco47III 152 AGCGCT AGC^GCT Eco81I 870 CCTGAGG CC^T[ACGT]AGG EcoRI 1156 GAATTC G^AATTC EcoT14I 759 CCAAGG C^C[AT][AT]GG FbaI 446 TGATCA T^GATCA HgaI 953 GCGTC GACGC(5/10) Ksp632I 430 CTCTTC CTCTTC(1/4) KspI 183 CCGCGG CCGC^GG MaeIII 1004 GTGAC ^GT[ACGT]AC MamI 447 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 866 GGATCC [GA]^GATC[CT] PpuMI 722 GGGACCT [GA]G^G[AT]CC[CT] PstI 1087 CTGCAG CTGCA^G PvuII 18 CAGCTG CAG^CTG SacII 183 CCGCGG CCGC^GG SexAI 725 ACCTGGT A^CC[AT]GGT SfcI 1087 CTGCAG C^T[GA][CT]AG SgrAI 291 CACCGGCG C[GA]^CCGG[CT]G SspBI 644 TGTACA T^GTACA StyI 759 CCAAGG C^C[AT][AT]GG Tsp45I 1004 GTGAC ^GT[GC]AC Tsp509I 1157 AATT ^AATT XhoII 866 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 494 TCCGGA T^CCGGA 956 TCCGGA AfaI 582 GTAC GT^AC 645 GTAC ApaI 24 GGGCCC GGGCC^C 506 GGGCCC AspI 265 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 724 GACCTGGTC BanII 24 GGGCCC G[GA]GC[CT]^C 506 GGGCCC BbsI 109 GTCTTC GAAGAC(2/6) 787 GTCTTC BpuAI 109 GTCTTC GAAGAC(2/6) 787 GTCTTC BsaWI 494 TCCGGA [AT]^CCGG[AT] 956 TCCGGA BseAI 494 TCCGGA T^CCGGA 956 TCCGGA BseRI 160 GAGGAG GAGGAG(10/8) 235 GAGGAG BspEI 494 TCCGGA T^CCGGA 956 TCCGGA BspHI 467 TCATGA T^CATGA 1187 TCATGA EcoNI 36 CCTGAACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 510 CCTGCCCCAGG HinfI 676 GACTC G^A[ACGT]TC 914 GACTC MroI 494 TCCGGA T^CCGGA 956 TCCGGA MslI 255 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 792 CATCCTCATG PflFI 265 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 724 GACCTGGTC PleI 676 GACTC GAGTC(4/5) 914 GACTC RcaI 467 TCATGA T^CATGA 1187 TCATGA RsaI 582 GTAC GT^AC 645 GTAC SfiI 240 GGCCACTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1071 GGCCTCGTTGGCC SmaI 27 CCCGGG CCC^GGG 1145 CCCGGG StuI 762 AGGCCT AGG^CCT 1070 AGGCCT Tth111I 265 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 724 GACCTGGTC XmaCI 27 CCCGGG C^CCGGG 1145 CCCGGG XmaI 27 CCCGGG C^CCGGG 1145 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 76 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaI BalI BanI BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfoI TseI TspRI