(Circular) (Six-base) MAPSORT of: pFN21AE5511.seq Check: 5614 from: 1 to: 5929 pFN21AE5511 5929 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3739 278 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3272 1937 Size: 1335 4594 Acc65I G'GTAC_C Cuts at: 3250 3250 Size: 5929 AclI AA'CG_TT Cuts at: 1563 4056 4429 1563 Size: 2493 373 3063 Fragments arranged by size: 3063 2493 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 2552 3992 5376 5644 1632 Size: 535 385 1440 1384 268 1917 Fragments arranged by size: 1917 1440 1384 535 385 268 AfeI AGC'GCT Cuts at: 2159 2159 Size: 5929 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5732 AflIII A'CryG_T Cuts at: 1203 4828 5805 1203 Size: 3625 977 1327 Fragments arranged by size: 3625 1327 977 AgeI A'CCGG_T Cuts at: 4760 4760 Size: 5929 AhdI GACnn_n'nnGTC Cuts at: 4658 4658 Size: 5929 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5929 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5929 AlwNI CAG_nnn'CTG Cuts at: 3672 5244 3672 Size: 1572 4357 ApaI G_GGCC'C Cuts at: 2033 2515 2033 Size: 482 5447 ApaLI G'TGCA_C Cuts at: 3986 5142 3986 Size: 1156 4773 ApoI r'AATT_y Cuts at: 1493 3161 3238 3464 3616 5725 1493 Size: 1668 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 1668 226 152 77 AseI AT'TA_AT Cuts at: 160 1032 4483 160 Size: 872 3451 1606 Fragments arranged by size: 3451 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2250 3150 3254 1870 Size: 73 89 218 900 104 4545 Fragments arranged by size: 4545 900 218 104 89 73 BamHI G'GATC_C Cuts at: 2871 3259 3626 2871 Size: 388 367 5174 Fragments arranged by size: 5174 388 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2105 2300 2318 3021 3250 Size: 325 217 627 318 195 18 703 229 Cuts at: 3250 4705 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 703 627 325 318 229 217 195 18 BanII G_rGCy'C Cuts at: 1391 2033 2515 3248 1391 Size: 642 482 733 4072 Fragments arranged by size: 4072 733 642 482 BbeI G_GCGC'C Cuts at: 2109 2304 3025 2109 Size: 195 721 5013 Fragments arranged by size: 5013 721 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2107 2785 928 Size: 971 208 678 4072 Fragments arranged by size: 4072 971 678 208 BbvCI CC'TCA_GC Cuts at: 2641 2641 Size: 5929 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4120 4154 1903 Size: 34 2183 34 3678 Fragments arranged by size: 3678 2183 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3821 5031 3821 Size: 1210 4719 BclI T'GATC_A Cuts at: 1548 2451 1548 Size: 903 5026 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 2568 3074 Size: 107 122 71 631 605 580 316 506 Cuts at: 3074 3083 4540 136 Size: 9 1457 1525 Fragments arranged by size: 1525 1457 631 605 580 506 316 122 107 71 9 BglII A'GATC_T Cuts at: 5924 5924 Size: 5929 BlpI GC'TnA_GC Cuts at: 3346 3346 Size: 5929 Bme1580I G_kGCm'C Cuts at: 1790 2033 2321 2515 3990 5146 1790 Size: 243 288 194 1475 1156 2573 Fragments arranged by size: 2573 1475 1156 288 243 194 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5929 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3660 4618 442 Size: 416 53 83 399 24 2243 958 1753 Fragments arranged by size: 2243 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5929 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3061 3061 Size: 5929 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2462 2689 4589 1239 Size: 908 315 227 1900 2579 Fragments arranged by size: 2579 1900 908 315 227 Bpu10I CC'TnA_GC Cuts at: 2201 2641 2201 Size: 440 5489 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4054 4919 5217 4054 Size: 865 298 4766 Fragments arranged by size: 4766 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3206 4592 882 Size: 617 1707 1386 2219 Fragments arranged by size: 2219 1707 1386 617 BsaAI yAC'GTr Cuts at: 493 1204 3720 493 Size: 711 2516 2702 Fragments arranged by size: 2702 2516 711 BsaBI GATnn'nnATC Cuts at: 2456 5923 2456 Size: 3467 2462 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 2301 3022 3736 Size: 53 83 186 936 573 195 721 714 Cuts at: 3736 4118 4753 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 721 714 635 573 382 195 186 83 53 BsaWI w'CCGG_w Cuts at: 2499 2961 4362 4760 5034 5181 5592 2499 Size: 462 1401 398 274 147 411 2836 Fragments arranged by size: 2836 1401 462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5704 Fragments arranged by size: 5704 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2255 1186 Size: 994 75 4860 Fragments arranged by size: 4860 994 75 BseYI C'CCAG_C Cuts at: 2025 2098 2160 2286 5132 5628 2025 Size: 73 62 126 2846 496 2326 Fragments arranged by size: 2846 2326 496 126 73 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5356 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3379 4140 5168 1540 Size: 275 186 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 3990 4075 5146 2091 Size: 1899 85 1071 2874 Fragments arranged by size: 2874 1899 1071 85 BsmI GAATG_Cn' Cuts at: 3440 3533 3440 Size: 93 5836 BsmBI CGTCTCn'nnnn_ Cuts at: 2497 2497 Size: 5929 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2091 2321 2515 3248 3990 4075 Size: 399 243 58 230 194 733 742 85 Cuts at: 4075 5146 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 733 399 243 230 194 85 58 BspEI T'CCGG_A Cuts at: 2499 2961 5592 2499 Size: 462 2631 2836 Fragments arranged by size: 2836 2631 462 BspHI T'CATG_A Cuts at: 2472 3192 3818 5548 2472 Size: 720 626 1730 2853 Fragments arranged by size: 2853 1730 720 626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2103 3284 844 Size: 1259 1181 3489 Fragments arranged by size: 3489 1259 1181 BsrBI CCG'CTC Cuts at: 1394 1823 3816 1394 Size: 429 1993 3507 Fragments arranged by size: 3507 1993 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2912 4424 4598 65 Size: 1183 1664 1512 174 1396 Fragments arranged by size: 1664 1512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2255 2297 2567 3024 4573 4760 1700 Size: 110 445 42 270 457 1549 187 2869 Fragments arranged by size: 2869 1549 457 445 270 187 110 42 BsrGI T'GTAC_A Cuts at: 96 2649 96 Size: 2553 3376 BssHII G'CGCG_C Cuts at: 1925 2057 1925 Size: 132 5797 BssSI C'ACGA_G Cuts at: 2138 3989 5001 2138 Size: 1851 1012 3066 Fragments arranged by size: 3066 1851 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5929 BstBI TT'CG_AA Cuts at: 4733 4733 Size: 5929 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5929 BstXI CCAn_nnnn'nTGG Cuts at: 3145 3145 Size: 5929 BstYI r'GATC_y Cuts at: 1972 2871 3259 3626 4011 4028 5480 5566 5578 Size: 899 388 367 385 17 1452 86 12 Cuts at: 5578 5924 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 899 388 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2876 2876 Size: 5929 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 513 Size: 552 939 184 4254 Fragments arranged by size: 4254 939 552 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2446 487 Size: 683 56 1220 3970 Fragments arranged by size: 3970 1220 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3445 4239 4259 2552 Size: 893 794 20 4222 Fragments arranged by size: 4222 893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5929 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5894 DraI TTT'AAA Cuts at: 3233 3233 Size: 5929 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4936 809 Size: 507 3620 1802 Fragments arranged by size: 3620 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2073 2175 Size: 54 1425 50 275 18 181 62 102 Cuts at: 2175 2191 2565 3084 3114 3376 4265 5894 8 Size: 16 374 519 30 262 889 1629 43 Fragments arranged by size: 1629 1425 889 519 374 275 262 181 102 62 54 50 43 30 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3376 1537 Size: 275 1564 4090 Fragments arranged by size: 4090 1564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2441 3859 1353 Size: 1088 1418 3423 Fragments arranged by size: 3423 1418 1088 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2219 2267 4516 4890 5036 387 Size: 1064 138 630 48 2249 374 146 1280 Fragments arranged by size: 2249 1280 1064 630 374 146 138 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2167 2462 2552 2689 3992 4589 Size: 393 515 20 295 90 137 1303 597 Cuts at: 4589 5376 5644 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1303 787 597 515 393 295 268 137 90 20 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2519 2045 Size: 474 5455 EcoO109I rG'GnC_Cy Cuts at: 2029 2511 2512 2728 2029 Size: 482 1 216 5230 Fragments arranged by size: 5230 482 216 1 EcoRI G'AATT_C Cuts at: 3161 3238 3616 3161 Size: 77 378 5474 Fragments arranged by size: 5474 378 77 FspI TGC'GCA Cuts at: 4435 4435 Size: 5929 HaeII r_GCGC'y Cuts at: 1678 2109 2146 2161 2173 2304 2449 3025 5076 Size: 431 37 15 12 131 145 576 2051 Cuts at: 5076 1678 Size: 2531 Fragments arranged by size: 2531 2051 576 431 145 131 37 15 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2361 2370 2393 4570 Size: 32 135 32 766 23 9 23 2177 Cuts at: 4570 4602 4644 4676 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2177 766 135 42 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3273 3519 669 Size: 988 281 1335 246 3079 Fragments arranged by size: 3079 1335 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5929 HpaI GTT'AAC Cuts at: 3519 3519 Size: 5929 KasI G'GCGC_C Cuts at: 2105 2300 3021 2105 Size: 195 721 5013 Fragments arranged by size: 5013 721 195 KpnI G_GTAC'C Cuts at: 3254 3254 Size: 5929 MfeI C'AATT_G Cuts at: 3528 3528 Size: 5929 MluI A'CGCG_T Cuts at: 5805 5805 Size: 5929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5043 5227 1305 Size: 3738 184 2007 Fragments arranged by size: 3738 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2177 3086 3116 10 Size: 54 1425 343 345 909 30 2823 Fragments arranged by size: 2823 1425 909 345 343 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2801 3887 4246 4405 518 Size: 648 1098 537 1086 359 159 2042 Fragments arranged by size: 2042 1098 1086 648 537 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2190 2491 2892 2970 3345 4022 5170 Size: 419 165 301 401 78 375 677 1148 Cuts at: 5170 5415 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 419 401 375 301 245 165 78 NaeI GCC'GGC Cuts at: 2257 2569 3026 2257 Size: 312 457 5160 Fragments arranged by size: 5160 457 312 NarI GG'CG_CC Cuts at: 2106 2301 3022 2106 Size: 195 721 5013 Fragments arranged by size: 5013 721 195 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4438 Fragments arranged by size: 4438 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5929 NgoMIV G'CCGG_C Cuts at: 2255 2567 3024 2255 Size: 312 457 5160 Fragments arranged by size: 5160 457 312 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5929 NotI GC'GGCC_GC Cuts at: 3376 3376 Size: 5929 NspI r_CATG'y Cuts at: 1132 1229 3287 4832 1132 Size: 97 2058 1545 2229 Fragments arranged by size: 2229 2058 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5929 PciI A'CATG_T Cuts at: 4828 4828 Size: 5929 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5929 PfoI T'CCnGG_A Cuts at: 2439 2439 Size: 5929 PmeI GTTT'AAAC Cuts at: 3233 3233 Size: 5929 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3998 4030 5511 5543 3998 Size: 32 1481 32 4384 Fragments arranged by size: 4384 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2728 2728 Size: 5929 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5929 PsiI TTA'TAA Cuts at: 3499 3499 Size: 5929 PspOMI G'GGCC_C Cuts at: 2029 2511 2029 Size: 482 5447 PstI C_TGCA'G Cuts at: 830 3096 3281 830 Size: 2266 185 3478 Fragments arranged by size: 3478 2266 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5929 PvuII CAG'CTG Cuts at: 2025 2025 Size: 5929 SacII CC_GC'GG Cuts at: 2191 2191 Size: 5929 SalI G'TCGA_C Cuts at: 1936 3271 1936 Size: 1335 4594 SbfI CC_TGCA'GG Cuts at: 3281 3281 Size: 5929 ScaI AGT'ACT Cuts at: 4177 5889 4177 Size: 1712 4217 SexAI A'CCwGG_T Cuts at: 2730 2730 Size: 5929 SfcI C'TryA_G Cuts at: 826 1045 3092 3277 4412 5093 5284 826 Size: 219 2047 185 1135 681 191 1471 Fragments arranged by size: 2047 1471 1135 681 219 191 185 SfiI GGCCn_nnn'nGGCC Cuts at: 2252 3083 2252 Size: 831 5098 SfoI GGC'GCC Cuts at: 2107 2302 3023 2107 Size: 195 721 5013 Fragments arranged by size: 5013 721 195 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5929 SgrAI Cr'CCGG_yG Cuts at: 1700 2297 1700 Size: 597 5332 SmaI CCC'GGG Cuts at: 1872 2034 3152 3256 1872 Size: 162 1118 104 4545 Fragments arranged by size: 4545 1118 162 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 4033 4934 5196 820 Size: 197 926 2090 901 262 1553 Fragments arranged by size: 2090 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3720 493 Size: 3227 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5929 SphI G_CATG'C Cuts at: 1132 3287 1132 Size: 2155 3774 SrfI GCCC'GGGC Cuts at: 2034 2034 Size: 5929 SspI AAT'ATT Cuts at: 5 52 3853 5 Size: 47 3801 2081 Fragments arranged by size: 3801 2081 47 StuI AGG'CCT Cuts at: 1517 2769 3077 1517 Size: 1252 308 4369 Fragments arranged by size: 4369 1252 308 StyI C'CwwG_G Cuts at: 513 1065 2004 2764 3368 513 Size: 552 939 760 604 3074 Fragments arranged by size: 3074 939 760 604 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2394 2795 3956 3973 4126 4311 4756 Size: 1255 814 401 1161 17 153 185 445 Cuts at: 4756 325 Size: 1498 Fragments arranged by size: 1498 1255 1161 814 445 401 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2586 2649 4175 Size: 274 80 33 51 1443 609 63 1526 Cuts at: 4175 5887 96 Size: 1712 138 Fragments arranged by size: 1712 1526 1443 609 274 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2879 2911 5889 5921 2879 Size: 32 2978 32 2887 Fragments arranged by size: 2978 2887 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2273 2732 1316 Size: 957 459 4513 Fragments arranged by size: 4513 957 459 XbaI T'CTAG_A Cuts at: 3265 3265 Size: 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5929 XmaI C'CCGG_G Cuts at: 1870 2032 3150 3254 1870 Size: 162 1118 104 4545 Fragments arranged by size: 4545 1118 162 104 XmnI GAAnn'nnTTC Cuts at: 4058 4058 Size: 5929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3737 276 Size: 53 83 186 936 2203 2468 Fragments arranged by size: 2468 2203 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SwaI XcmI