(Linear) (Six-base) MAPSORT of: KIEE5511.seq Check: 5817 from: 1 to: 1224 KIEE5511.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 162 547 1224 Size: 162 385 677 Fragments arranged by size: 677 385 162 AfeI AGC'GCT Cuts at: 0 154 1224 Size: 154 1070 ApaI G_GGCC'C Cuts at: 0 28 510 1224 Size: 28 482 714 Fragments arranged by size: 714 482 28 ApoI r'AATT_y Cuts at: 0 1156 1224 Size: 1156 68 AvaI C'yCGr_G Cuts at: 0 27 245 1145 1224 Size: 27 218 900 79 Fragments arranged by size: 900 218 79 27 BamHI G'GATC_C Cuts at: 0 866 1224 Size: 866 358 BanI G'GyrC_C Cuts at: 0 100 295 313 1016 1224 Size: 100 195 18 703 208 Fragments arranged by size: 703 208 195 100 18 BanII G_rGCy'C Cuts at: 0 28 510 1224 Size: 28 482 714 Fragments arranged by size: 714 482 28 BbeI G_GCGC'C Cuts at: 0 104 299 1020 1224 Size: 104 195 721 204 Fragments arranged by size: 721 204 195 104 BbsI GAAGACnn'nnnn_ Cuts at: 0 102 780 1224 Size: 102 678 444 Fragments arranged by size: 678 444 102 BbvCI CC'TCA_GC Cuts at: 0 636 1224 Size: 636 588 BclI T'GATC_A Cuts at: 0 446 1224 Size: 446 778 BglI GCCn_nnn'nGGC Cuts at: 0 247 563 1069 1078 1224 Size: 247 316 506 9 146 Fragments arranged by size: 506 316 247 146 9 Bme1580I G_kGCm'C Cuts at: 0 28 316 510 1224 Size: 28 288 194 714 Fragments arranged by size: 714 288 194 28 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1056 1224 Size: 1056 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 457 684 1224 Size: 142 315 227 540 Fragments arranged by size: 540 315 227 142 Bpu10I CC'TnA_GC Cuts at: 0 196 636 1224 Size: 196 440 588 Fragments arranged by size: 588 440 196 BsaI GGTCTCn'nnnn_ Cuts at: 0 1201 1224 Size: 1201 23 BsaBI GATnn'nnATC Cuts at: 0 451 1224 Size: 451 773 BsaHI Gr'CG_yC Cuts at: 0 101 296 1017 1224 Size: 101 195 721 207 Fragments arranged by size: 721 207 195 101 BsaWI w'CCGG_w Cuts at: 0 494 956 1224 Size: 494 462 268 Fragments arranged by size: 494 462 268 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 175 250 1224 Size: 175 75 974 Fragments arranged by size: 974 175 75 BseYI C'CCAG_C Cuts at: 0 20 93 155 281 1224 Size: 20 73 62 126 943 Fragments arranged by size: 943 126 73 62 20 BsiHKAI G_wGCw'C Cuts at: 0 86 1224 Size: 86 1138 BsmBI CGTCTCn'nnnn_ Cuts at: 0 492 1224 Size: 492 732 Bsp1286I G_dGCh'C Cuts at: 0 28 86 316 510 1224 Size: 28 58 230 194 714 Fragments arranged by size: 714 230 194 58 28 BspEI T'CCGG_A Cuts at: 0 494 956 1224 Size: 494 462 268 Fragments arranged by size: 494 462 268 BspHI T'CATG_A Cuts at: 0 467 1187 1224 Size: 467 720 37 Fragments arranged by size: 720 467 37 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 98 1224 Size: 98 1126 BsrDI GCAATG_nn' Cuts at: 0 907 1224 Size: 907 317 BsrFI r'CCGG_y Cuts at: 0 250 292 562 1019 1224 Size: 250 42 270 457 205 Fragments arranged by size: 457 270 250 205 42 BsrGI T'GTAC_A Cuts at: 0 644 1224 Size: 644 580 BssHII G'CGCG_C Cuts at: 0 52 1224 Size: 52 1172 BssSI C'ACGA_G Cuts at: 0 133 1224 Size: 133 1091 BstXI CCAn_nnnn'nTGG Cuts at: 0 1140 1224 Size: 1140 84 BstYI r'GATC_y Cuts at: 0 866 1224 Size: 866 358 Bsu36I CC'TnA_GG Cuts at: 0 871 1224 Size: 871 353 BtgI C'CryG_G Cuts at: 0 183 1224 Size: 183 1041 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 441 1224 Size: 441 783 BtsI GCAGTG_nn' Cuts at: 0 547 1224 Size: 547 677 EaeI y'GGCC_r Cuts at: 0 6 68 170 186 560 1079 1109 1224 Size: 6 62 102 16 374 519 30 115 Fragments arranged by size: 519 374 115 102 62 30 16 6 EarI CTCTTCn'nnn_ Cuts at: 0 436 1224 Size: 436 788 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 214 262 1224 Size: 214 48 962 Fragments arranged by size: 962 214 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 162 457 547 684 1224 Size: 142 20 295 90 137 540 Fragments arranged by size: 540 295 142 137 90 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 40 514 1224 Size: 40 474 710 Fragments arranged by size: 710 474 40 EcoO109I rG'GnC_Cy Cuts at: 0 24 506 507 723 1224 Size: 24 482 1 216 501 Fragments arranged by size: 501 482 216 24 1 EcoRI G'AATT_C Cuts at: 0 1156 1224 Size: 1156 68 HaeII r_GCGC'y Cuts at: 0 104 141 156 168 299 444 1020 1224 Size: 104 37 15 12 131 145 576 204 Fragments arranged by size: 576 204 145 131 104 37 15 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 333 356 365 388 1224 Size: 333 23 9 23 836 Fragments arranged by size: 836 333 23 23 9 KasI G'GCGC_C Cuts at: 0 100 295 1016 1224 Size: 100 195 721 208 Fragments arranged by size: 721 208 195 100 MscI TGG'CCA Cuts at: 0 172 1081 1111 1224 Size: 172 909 30 113 Fragments arranged by size: 909 172 113 30 MslI CAynn'nnrTG Cuts at: 0 259 796 1224 Size: 259 537 428 Fragments arranged by size: 537 428 259 MspA1I CmG'CkG Cuts at: 0 20 185 486 887 965 1224 Size: 20 165 301 401 78 259 Fragments arranged by size: 401 301 259 165 78 20 NaeI GCC'GGC Cuts at: 0 252 564 1021 1224 Size: 252 312 457 203 Fragments arranged by size: 457 312 252 203 NarI GG'CG_CC Cuts at: 0 101 296 1017 1224 Size: 101 195 721 207 Fragments arranged by size: 721 207 195 101 NgoMIV G'CCGG_C Cuts at: 0 250 562 1019 1224 Size: 250 312 457 205 Fragments arranged by size: 457 312 250 205 PfoI T'CCnGG_A Cuts at: 0 434 1224 Size: 434 790 PpuMI rG'GwC_Cy Cuts at: 0 723 1224 Size: 723 501 PspOMI G'GGCC_C Cuts at: 0 24 506 1224 Size: 24 482 718 Fragments arranged by size: 718 482 24 PstI C_TGCA'G Cuts at: 0 1091 1224 Size: 1091 133 PvuII CAG'CTG Cuts at: 0 20 1224 Size: 20 1204 SacII CC_GC'GG Cuts at: 0 186 1224 Size: 186 1038 SexAI A'CCwGG_T Cuts at: 0 725 1224 Size: 725 499 SfcI C'TryA_G Cuts at: 0 1087 1224 Size: 1087 137 SfiI GGCCn_nnn'nGGCC Cuts at: 0 247 1078 1224 Size: 247 831 146 Fragments arranged by size: 831 247 146 SfoI GGC'GCC Cuts at: 0 102 297 1018 1224 Size: 102 195 721 206 Fragments arranged by size: 721 206 195 102 SgrAI Cr'CCGG_yG Cuts at: 0 292 1224 Size: 292 932 SmaI CCC'GGG Cuts at: 0 29 1147 1224 Size: 29 1118 77 Fragments arranged by size: 1118 77 29 SrfI GCCC'GGGC Cuts at: 0 29 1224 Size: 29 1195 StuI AGG'CCT Cuts at: 0 764 1072 1224 Size: 764 308 152 Fragments arranged by size: 764 308 152 StyI C'CwwG_G Cuts at: 0 759 1224 Size: 759 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 389 790 1224 Size: 389 401 434 Fragments arranged by size: 434 401 389 TatI w'GTAC_w Cuts at: 0 581 644 1224 Size: 581 63 580 Fragments arranged by size: 581 580 63 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 874 906 1224 Size: 874 32 318 Fragments arranged by size: 874 318 32 Tth111I GACn'n_nGTC Cuts at: 0 268 727 1224 Size: 268 459 497 Fragments arranged by size: 497 459 268 XmaI C'CCGG_G Cuts at: 0 27 1145 1224 Size: 27 1118 79 Fragments arranged by size: 1118 79 27 Enzymes that do cut: AcuI AfeI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV PfoI PpuMI PspOMI PstI PvuII SacII SexAI SfcI SfiI SfoI SgrAI SmaI SrfI StuI StyI TaqII TatI TstI Tth111I XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaXI BsgI BsiEI BsiWI BsmI BsrBI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SgfI SmlI SnaBI SpeI SphI SspI SwaI XbaI XcmI XhoI XmnI ZraI