################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI KpnI Ksp632I KspI MaeI MaeII MaeIII MfeI MluI MroI MseI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 298 GGCGCC G[GA]^CG[CT]C AlwI 78 GGATC GGATC(4/5) Aor51HI 185 AGCGCT AGC^GCT AspI 84 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 100 CCCGAG C^[CT]CG[GA]G AvaII 384 GGACC G^G[AT]CC BalI 372 TGGCCA TGG^CCA BbeI 298 GGCGCC GGCGC^C BglI 58 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 15 GCTGAGC GC^T[ACGT]AGC BmyI 449 GTGCCC G[AGT]GC[ACT]^C BpmI 355 CTGGAG CTGGAG(16/14) Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC BsaBI 210 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 298 GGCGCC G[GA]^CG[CT]C BsaI 145 GAGACC GGTCTC(1/5) BsgI 181 GTGCAG GTGCAG(16/14) BsiEI 222 CGGCCG CG[GA][CT]^CG BsoBI 100 CCCGAG C^[CT]CG[GA]G Bsp1286I 449 GTGCCC G[AGT]GC[ACT]^C BsrBI 471 CCGCTC CCGCTC(-3/-3) BsrI 375 CCAGT ACTGG(1/-1) BstYI 78 GGATCT [GA]^GATC[CT] CelII 15 GCTGAGC GC^T[ACGT]AGC DsaI 225 CCGTGG C^C[GA][CT]GG EagI 222 CGGCCG C^GGCCG EclXI 222 CGGCCG C^GGCCG Eco47III 185 AGCGCT AGC^GCT Eco52I 222 CGGCCG C^GGCCG EcoNI 117 CCTGCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 305 CCAAGG C^C[AT][AT]GG Hin1I 298 GGCGCC G[GA]^CG[CT]C HphI 281 TCACC GGTGA(8/7) KasI 298 GGCGCC G^GCGCC MamI 210 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 278 TCTTC GAAGA(8/7) MflI 78 GGATCT [GA]^GATC[CT] MluNI 372 TGGCCA TGG^CCA MscI 372 TGGCCA TGG^CCA NarI 298 GGCGCC GG^CGCC PflFI 84 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 232 CTGCAG CTGCA^G SfcI 232 CTGCAG C^T[GA][CT]AG SfoI 298 GGCGCC GGC^GCC StuI 56 AGGCCT AGG^CCT StyI 305 CCAAGG C^C[AT][AT]GG Tth111I 84 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 78 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 240 GTAC GT^AC 262 GTAC AlwNI 172 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG 340 CAGGAGCTG BcnI 51 CCGGG CC^[GC]GG 445 CCCGG BsmFI 468 GTCCC GGGAC(10/14) 480 GTCCC Cfr13I 98 GGCCC G^G[ACGT]CC 384 GGACC DpnII 79 GATC ^GATC 405 GATC EaeI 222 CGGCCG [CT]^GGCC[GA] 372 TGGCCA FauI 470 CCCGC CCCGC(4/6) 258 GCGGG HapII 51 CCGG C^CGG 446 CCGG HpaII 51 CCGG C^CGG 446 CCGG MboI 79 GATC ^GATC 405 GATC MspI 51 CCGG C^CGG 446 CCGG NciI 51 CCGGG CC^[GC]GG 445 CCCGG NdeII 79 GATC ^GATC 405 GATC PvuII 244 CAGCTG CAG^CTG 415 CAGCTG RsaI 240 GTAC GT^AC 262 GTAC Sau3AI 79 GATC ^GATC 405 GATC Sau96I 98 GGCCC G^G[ACGT]CC 384 GGACC SfaNI 204 GATGC GCATC(5/9) 215 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BanI BbvI BsaJI BseRI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I CfoI DdeI EcoRII Fnu4HI FokI HaeII HaeIII HhaI HinP1I ItaI MnlI MspA1I MvaI MwoI NlaIII NlaIV PflMI ScrFI TseI TspRI Van91I