(Linear) (Six-base) MAPSORT of: KIEE6114.seq Check: 6257 from: 1 to: 501 KIEE6114.orf With 182 enzymes: SgfI * December 5, 2014 13:24 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 501 Size: 322 179 AfeI AGC'GCT Cuts at: 0 187 501 Size: 187 314 AlwNI CAG_nnn'CTG Cuts at: 0 177 345 501 Size: 177 168 156 Fragments arranged by size: 177 168 156 AvaI C'yCGr_G Cuts at: 0 100 501 Size: 100 401 BanI G'GyrC_C Cuts at: 0 298 429 448 501 Size: 298 131 19 53 Fragments arranged by size: 298 131 53 19 BbeI G_GCGC'C Cuts at: 0 302 501 Size: 302 199 BglI GCCn_nnn'nGGC Cuts at: 0 64 501 Size: 64 437 BlpI GC'TnA_GC Cuts at: 0 16 501 Size: 16 485 Bme1580I G_kGCm'C Cuts at: 0 453 501 Size: 453 48 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 129 501 Size: 129 372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 376 501 Size: 376 125 BsaI GGTCTCn'nnnn_ Cuts at: 0 139 501 Size: 139 362 BsaBI GATnn'nnATC Cuts at: 0 214 501 Size: 214 287 BsaHI Gr'CG_yC Cuts at: 0 299 501 Size: 299 202 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 118 139 325 501 Size: 49 69 21 186 176 Fragments arranged by size: 186 176 69 49 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 501 Size: 202 299 BsiEI CG_ry'CG Cuts at: 0 225 501 Size: 225 276 Bsp1286I G_dGCh'C Cuts at: 0 453 501 Size: 453 48 BsrBI CCG'CTC Cuts at: 0 473 501 Size: 473 28 BstYI r'GATC_y Cuts at: 0 78 501 Size: 78 423 BtgI C'CryG_G Cuts at: 0 225 501 Size: 225 276 BtsI GCAGTG_nn' Cuts at: 0 172 501 Size: 172 329 EaeI y'GGCC_r Cuts at: 0 222 372 501 Size: 222 150 129 Fragments arranged by size: 222 150 129 EagI C'GGCC_G Cuts at: 0 222 501 Size: 222 279 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 322 376 501 Size: 322 54 125 Fragments arranged by size: 322 125 54 EcoNI CCTnn'n_nnAGG Cuts at: 0 121 501 Size: 121 380 HaeII r_GCGC'y Cuts at: 0 51 189 302 355 501 Size: 51 138 113 53 146 Fragments arranged by size: 146 138 113 53 51 KasI G'GCGC_C Cuts at: 0 298 501 Size: 298 203 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 11 501 Size: 11 490 MscI TGG'CCA Cuts at: 0 374 501 Size: 374 127 MspA1I CmG'CkG Cuts at: 0 246 258 270 417 501 Size: 246 12 12 147 84 Fragments arranged by size: 246 147 84 12 12 NarI GG'CG_CC Cuts at: 0 299 501 Size: 299 202 PasI CC'CwG_GG Cuts at: 0 366 501 Size: 366 135 PflMI CCAn_nnn'nTGG Cuts at: 0 177 381 396 501 Size: 177 204 15 105 Fragments arranged by size: 204 177 105 15 PstI C_TGCA'G Cuts at: 0 236 501 Size: 236 265 PvuII CAG'CTG Cuts at: 0 246 417 501 Size: 246 171 84 Fragments arranged by size: 246 171 84 SfcI C'TryA_G Cuts at: 0 232 501 Size: 232 269 SfoI GGC'GCC Cuts at: 0 300 501 Size: 300 201 StuI AGG'CCT Cuts at: 0 58 501 Size: 58 443 StyI C'CwwG_G Cuts at: 0 305 501 Size: 305 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 148 358 501 Size: 148 210 143 Fragments arranged by size: 210 148 143 Tth111I GACn'n_nGTC Cuts at: 0 87 501 Size: 87 414 Enzymes that do cut: AcuI AfeI AlwNI AvaI BanI BbeI BglI BlpI Bme1580I BplI BpmI BsaI BsaBI BsaHI BseRI BsgI BsiEI Bsp1286I BsrBI BstYI BtgI BtsI EaeI EagI Eco57MI EcoNI HaeII KasI MmeI MscI MspA1I NarI PasI PflMI PstI PvuII SfcI SfoI StuI StyI TaqII Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaWI BsaXI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI