################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MluI MroI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 82 CGCG CG^CG Asp700I 72 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1132 GAGCTC G[AT]GC[AT]^C AvaII 621 GGACC G^G[AT]CC BanI 163 GGTGCC G^G[CT][GA]CC BbvI 181 GCTGC GCAGC(8/12) BcnI 1175 CCCGG CC^[GC]GG BfaI 1207 CTAG C^TAG BsaAI 175 CACGTA [CT]AC^GT[GA] BsiHKAI 1132 GAGCTC G[AT]GC[AT]^C BsmBI 844 GAGACG CGTCTC(1/5) BspHI 279 TCATGA T^CATGA BssSI 530 CACGAG CACGAG(-5/-1) BstUI 82 CGCG CG^CG BstXI 611 CCAAACTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 436 GGATCT [GA]^GATC[CT] EarI 292 GAAGAG CTCTTC(1/4) EcoT14I 242 CCTTGG C^C[AT][AT]GG EcoT22I 732 ATGCAT ATGCA^T FauI 1031 CCCGC CCCGC(4/6) Fnu4HI 181 GCTGC GC^[ACGT]GC HapII 1176 CCGG C^CGG HpaII 1176 CCGG C^CGG HphI 902 TCACC GGTGA(8/7) ItaI 181 GCTGC GC^[ACGT]GC Ksp632I 292 GAAGAG CTCTTC(1/4) MaeI 1207 CTAG C^TAG MaeIII 856 GTCAC ^GT[ACGT]AC MfeI 44 CAATTG C^AATTG MflI 436 GGATCT [GA]^GATC[CT] MspI 1176 CCGG C^CGG MunI 44 CAATTG C^AATTG MvnI 82 CGCG CG^CG NciI 1175 CCCGG CC^[GC]GG NheI 1206 GCTAGC G^CTAGC NlaIV 163 GGTGCC GG[ACGT]^[ACGT]CC NsiI 732 ATGCAT ATGCA^T NspI 692 GCATGT [GA]CATG^[CT] PleI 832 GAGTC GAGTC(4/5) RcaI 279 TCATGA T^CATGA SacI 1132 GAGCTC GAGCT^C SmlI 1237 CTCAAG C^T[CT][GA]AG StyI 242 CCTTGG C^C[AT][AT]GG TaqI 1020 TCGA T^CGA TseI 181 GCTGC G^C[AT]GC Tsp45I 856 GTCAC ^GT[GC]AC TthHB8I 1020 TCGA T^CGA XhoII 436 GGATCT [GA]^GATC[CT] XmnI 72 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 246 GGATC GGATC(4/5) 436 GGATC BalI 128 TGGCCA TGG^CCA 980 TGGCCA BanII 819 GGGCTC G[GA]GC[CT]^C 1132 GAGCTC BciVI 156 GTATCC GTATCC(6/5) 405 GTATCC BmyI 819 GGGCTC G[AGT]GC[ACT]^C 1132 GAGCTC BsiYI 12 CCTGCCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1069 CCGATATCAGG BslI 12 CCTGCCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1069 CCGATATCAGG BsmI 521 GCATTC GAATGC(1/-1) 550 GCATTC Bsp1286I 819 GGGCTC G[AGT]GC[ACT]^C 1132 GAGCTC Cac8I 335 GCAGGC GC[ACGT]^[ACGT]GC 1206 GCTAGC Cfr13I 621 GGACC G^G[ACGT]CC 1045 GGCCC EaeI 128 TGGCCA [CT]^GGCC[GA] 980 TGGCCA EcoRV 325 GATATC GAT^ATC 1071 GATATC MaeII 176 ACGT A^CGT 1189 ACGT MluNI 128 TGGCCA TGG^CCA 980 TGGCCA MscI 128 TGGCCA TGG^CCA 980 TGGCCA MslI 530 CACGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 877 CACCTGAATG Sau96I 621 GGACC G^G[ACGT]CC 1045 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AcsI AluI ApoI BsaBI BsaI BsaJI BsgI BsmAI BsrI BssKI BstF5I BstNI DpnII EcoRII FokI HaeIII HinfI MamI MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI TfiI Tru9I Tsp509I TspRI