Restriction map for
pFN21AE5506
(Circular) (Six-base) MAPSORT of: pFN21AE5506.seq Check: 8045 from: 1 to: 5956
pFN21AE5506 5956 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 4019 5403 5671 1632
Size: 863 1524 1384 268 1917
Fragments arranged by size:
1917 1524 1384 863 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5956
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2066 2098 2714 2746 2066
Size: 32 616 32 5276
Fragments arranged by size:
5276 616 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3699 5271 3699
Size: 1572 4384
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 2635 2761 3155 3221 3265 3491 3643 5752
Size: 1142 126 394 66 44 226 152 2109
Cuts at: 5752 1493
Size: 1697
Fragments arranged by size:
2109 1697 1142 394 226 152 126 66
44
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 3281 1870
Size: 73 1338 4545
Fragments arranged by size:
4545 1338 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2168 3277 4732 618
Size: 325 217 627 381 1109 1455 1842
Fragments arranged by size:
1842 1455 1109 627 381 325 217
BanII G_rGCy'C
Cuts at: 1391 2828 3141 3275 1391
Size: 1437 313 134 4072
Fragments arranged by size:
4072 1437 313 134
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4985
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2172 2421 3848 5058 2172
Size: 249 1427 1210 3070
Fragments arranged by size:
3070 1427 1210 249
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BfrBI ATG'CAT
Cuts at: 2739 2739
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4567 136
Size: 107 122 71 631 605 2895 1525
Fragments arranged by size:
2895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5951 5951
Size: 5956
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 4017 5173 1790
Size: 2227 1156 2573
Fragments arranged by size:
2573 2227 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5956
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3106 3687 4645
Size: 416 53 83 399 24 1689 581 958
Cuts at: 4645 442
Size: 1753
Fragments arranged by size:
1753 1689 958 581 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3215 1056
Size: 2159 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4616 1239
Size: 3377 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3227 4081 4946 5244 3227
Size: 854 865 298 3939
Fragments arranged by size:
3939 865 854 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2428 2551 3197 4619 882
Size: 617 929 123 646 1422 2219
Fragments arranged by size:
2219 1422 929 646 617 123
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3747 493
Size: 711 978 1565 2702
Fragments arranged by size:
2702 1565 978 711
BsaBI GATnn'nnATC
Cuts at: 2409 2817 2953 5950 2409
Size: 408 136 2997 2415
Fragments arranged by size:
2997 2415 408 136
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3763 4145 4780 275
Size: 53 83 186 936 2230 382 635 1451
Fragments arranged by size:
2230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 2714 2744 1178
Size: 30 165 30 663 30 618 30 4390
Fragments arranged by size:
4390 663 618 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5956
BseYI C'CCAG_C
Cuts at: 2551 5159 5655 2551
Size: 2608 496 2852
Fragments arranged by size:
2852 2608 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2814 2829 3217 1163
Size: 573 1078 15 388 3902
Fragments arranged by size:
3902 1078 573 388 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3406 4167 5195 1540
Size: 275 186 1405 761 1028 2301
Fragments arranged by size:
2301 1405 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3141 4017 4102 5173 3141
Size: 876 85 1071 3924
Fragments arranged by size:
3924 1071 876 85
BsmI GAATG_Cn'
Cuts at: 2526 2555 3467 3560 2526
Size: 29 912 93 4922
Fragments arranged by size:
4922 912 93 29
BsmBI CGTCTCn'nnnn_
Cuts at: 2843 2843
Size: 5956
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2828 3141 3275 4017 4102 5173 1391
Size: 399 1038 313 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 1038 742 399 313 134 85
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 2284 3845 5575 2284
Size: 1561 1730 2665
Fragments arranged by size:
2665 1730 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3311 844
Size: 2467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4600 4787 1700
Size: 110 2790 187 2869
Fragments arranged by size:
2869 2790 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2535 4016 5028 2535
Size: 1481 1012 3463
Fragments arranged by size:
3463 1481 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5956
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstXI CCAn_nnnn'nTGG
Cuts at: 2623 2623
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2441 3286 3653 4038 4055 5507 5593 5605
Size: 469 845 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 845 469 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5217
Fragments arranged by size:
5217 683 56
BtsI GCAGTG_nn'
Cuts at: 3472 4266 4286 3472
Size: 794 20 5142
Fragments arranged by size:
5142 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5921
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2985 3403
Size: 54 1425 50 275 18 303 852 418
Cuts at: 3403 4292 5921 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 852 418 303 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3403 1537
Size: 275 1591 4090
Fragments arranged by size:
4090 1591 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2292 3886 1353
Size: 939 1594 3423
Fragments arranged by size:
3423 1594 939
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3139 3139
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2495 4019 4616 5403 5671 1239
Size: 393 863 1524 597 787 268 1524
Fragments arranged by size:
1524 1524 863 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
EcoRV GAT'ATC
Cuts at: 2332 3078 2332
Size: 746 5210
FspI TGC'GCA
Cuts at: 4462 4462
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 5103 1678
Size: 3425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2210 2242 2272 2304 2529
Size: 32 135 32 638 32 30 32 225
Cuts at: 2529 2561 2695 2727 3002 3034 4597 4629 4671
Size: 32 134 32 275 32 1563 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1563 638 275 225 135 134 42
32 32 32 32 32 32 32 32
32 30
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 2049 3555 2049
Size: 1506 4450
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2987 10
Size: 54 1425 343 303 852 2979
Fragments arranged by size:
2979 1425 852 343 303 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2539 2886 3914 4273 4432 518
Size: 648 1373 347 1028 359 159 2042
Fragments arranged by size:
2042 1373 1028 648 359 347 159
MspA1I CmG'CkG
Cuts at: 1606 3372 4049 5197 5442 1606
Size: 1766 677 1148 245 2120
Fragments arranged by size:
2120 1766 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5404
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 3211 1052
Size: 2159 3797
NotI GC'GGCC_GC
Cuts at: 3403 3403
Size: 5956
NsiI A_TGCA'T
Cuts at: 2741 2741
Size: 5956
NspI r_CATG'y
Cuts at: 1132 1229 2701 3314 4859 1132
Size: 97 1472 613 1545 2229
Fragments arranged by size:
2229 1545 1472 613 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5956
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5956
PfoI T'CCnGG_A
Cuts at: 3179 3179
Size: 5956
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2066 2098 2714 2746 4025 4057 5538 5570 2066
Size: 32 616 32 1279 32 1481 32 2452
Fragments arranged by size:
2452 1481 1279 616 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PstI C_TGCA'G
Cuts at: 830 3308 830
Size: 2478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
SacI G_AGCT'C
Cuts at: 3141 3141
Size: 5956
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 3304 4439 5120 5311 826
Size: 219 2259 1135 681 191 1471
Fragments arranged by size:
2259 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 3283 1872
Size: 1411 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3242 4060 4961 5223 820
Size: 197 926 1299 818 901 262 1553
Fragments arranged by size:
1553 1299 926 901 818 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5956
StyI C'CwwG_G
Cuts at: 513 1065 2247 3395 513
Size: 552 1182 1148 3074
Fragments arranged by size:
3074 1182 1148 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2189 2643 3983 4000 4153 4338 4783
Size: 1255 609 454 1340 17 153 185 445
Cuts at: 4783 325
Size: 1498
Fragments arranged by size:
1498 1340 1255 609 454 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5956
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 3281 1870
Size: 1411 4545
XmnI GAAnn'nnTTC
Cuts at: 2081 4085 2081
Size: 2004 3952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI