(Linear) (Six-base) MAPSORT of: KIEE5506.seq Check: 1715 from: 1 to: 1251 KIEE5506.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1251 Size: 490 761 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 61 93 709 741 1251 Size: 61 32 616 32 510 Fragments arranged by size: 616 510 61 32 32 ApoI r'AATT_y Cuts at: 0 630 756 1150 1216 1251 Size: 630 126 394 66 35 Fragments arranged by size: 630 394 126 66 35 BanI G'GyrC_C Cuts at: 0 163 1251 Size: 163 1088 BanII G_rGCy'C Cuts at: 0 823 1136 1251 Size: 823 313 115 Fragments arranged by size: 823 313 115 BciVI GTATCCnnnnn_n' Cuts at: 0 167 416 1251 Size: 167 249 835 Fragments arranged by size: 835 249 167 BfrBI ATG'CAT Cuts at: 0 734 1251 Size: 734 517 BmrI ACTGGGnnnn_n' Cuts at: 0 1101 1251 Size: 1101 150 BmtI G_CTAG'C Cuts at: 0 1210 1251 Size: 1210 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1222 1251 Size: 1222 29 BsaI GGTCTCn'nnnn_ Cuts at: 0 423 546 1192 1251 Size: 423 123 646 59 Fragments arranged by size: 646 423 123 59 BsaAI yAC'GTr Cuts at: 0 177 1251 Size: 177 1074 BsaBI GATnn'nnATC Cuts at: 0 404 812 948 1251 Size: 404 408 136 303 Fragments arranged by size: 408 404 303 136 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 61 91 709 739 1251 Size: 61 30 618 30 512 Fragments arranged by size: 618 512 61 30 30 BseYI C'CCAG_C Cuts at: 0 546 1251 Size: 546 705 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 809 824 1212 1251 Size: 809 15 388 39 Fragments arranged by size: 809 388 39 15 BsiHKAI G_wGCw'C Cuts at: 0 1136 1251 Size: 1136 115 BsmI GAATG_Cn' Cuts at: 0 521 550 1251 Size: 521 29 701 Fragments arranged by size: 701 521 29 BsmBI CGTCTCn'nnnn_ Cuts at: 0 838 1251 Size: 838 413 Bsp1286I G_dGCh'C Cuts at: 0 823 1136 1251 Size: 823 313 115 Fragments arranged by size: 823 313 115 BspHI T'CATG_A Cuts at: 0 279 1251 Size: 279 972 BssSI C'ACGA_G Cuts at: 0 530 1251 Size: 530 721 BstXI CCAn_nnnn'nTGG Cuts at: 0 618 1251 Size: 618 633 BstYI r'GATC_y Cuts at: 0 436 1251 Size: 436 815 EaeI y'GGCC_r Cuts at: 0 128 980 1251 Size: 128 852 271 Fragments arranged by size: 852 271 128 EarI CTCTTCn'nnn_ Cuts at: 0 287 1251 Size: 287 964 EcoICRI GAG'CTC Cuts at: 0 1134 1251 Size: 1134 117 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1251 Size: 490 761 EcoRV GAT'ATC Cuts at: 0 327 1073 1251 Size: 327 746 178 Fragments arranged by size: 746 327 178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 205 237 267 299 524 556 690 722 Size: 205 32 30 32 225 32 134 32 Cuts at: 722 997 1029 1251 Size: 275 32 222 Fragments arranged by size: 275 225 222 205 134 32 32 32 32 32 30 MfeI C'AATT_G Cuts at: 0 44 1251 Size: 44 1207 MscI TGG'CCA Cuts at: 0 130 982 1251 Size: 130 852 269 Fragments arranged by size: 852 269 130 MslI CAynn'nnrTG Cuts at: 0 534 881 1251 Size: 534 347 370 Fragments arranged by size: 534 370 347 NheI G'CTAG_C Cuts at: 0 1206 1251 Size: 1206 45 NsiI A_TGCA'T Cuts at: 0 736 1251 Size: 736 515 NspI r_CATG'y Cuts at: 0 696 1251 Size: 696 555 PfoI T'CCnGG_A Cuts at: 0 1174 1251 Size: 1174 77 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 61 93 709 741 1251 Size: 61 32 616 32 510 Fragments arranged by size: 616 510 61 32 32 SacI G_AGCT'C Cuts at: 0 1136 1251 Size: 1136 115 SmlI C'TyrA_G Cuts at: 0 1237 1251 Size: 1237 14 StyI C'CwwG_G Cuts at: 0 242 1251 Size: 242 1009 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 184 638 1251 Size: 184 454 613 Fragments arranged by size: 613 454 184 XmnI GAAnn'nnTTC Cuts at: 0 76 1251 Size: 76 1175 Enzymes that do cut: AcuI AloI ApoI BanI BanII BciVI BfrBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI BstXI BstYI EaeI EarI EcoICRI Eco57MI EcoRV Hin4I MfeI MscI MslI NheI NsiI NspI PfoI PpiI SacI SmlI StyI TaqII XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaHI BsaWI BseRI BsiEI BsiWI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI PacI PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI