################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BalI BamHI BbeI BbrPI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MseI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AlwNI 677 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 746 GAGCTC G[AT]GC[AT]^C AviII 407 TGCGCA TGC^GCA BanI 271 GGCACC G^G[CT][GA]CC BbsI 280 GAAGAC GAAGAC(2/6) BbvCI 251 CCTCAGC CCTCAGC(-5/-2) BciVI 223 GTATCC GTATCC(6/5) BclI 800 TGATCA T^GATCA BfaI 337 CTAG C^TAG BglI 667 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 280 GAAGAC GAAGAC(2/6) BsaAI 439 TACGTG [CT]AC^GT[GA] BsiHKAI 746 GAGCTC G[AT]GC[AT]^C BsmAI 502 GTCTC GTCTC(1/5) BsmBI 501 CGTCTC CGTCTC(1/5) BspHI 387 TCATGA T^CATGA BstF5I 570 GGATG GGATG(2/0) BstYI 821 AGATCC [GA]^GATC[CT] DraIII 400 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 189 GACAGCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 275 CCATGG C^C[GA][CT]GG EcoRI 76 GAATTC G^AATTC FbaI 800 TGATCA T^GATCA FokI 570 GGATG GGATG(9/13) FspI 407 TGCGCA TGC^GCA HincII 424 GTCAAC GT[CT]^[GA]AC HindII 424 GTCAAC GT[CT]^[GA]AC MaeI 337 CTAG C^TAG MaeII 440 ACGT A^CGT MflI 821 AGATCC [GA]^GATC[CT] NcoI 275 CCATGG C^CATGG NdeI 694 CATATG CA^TATG NspI 341 ACATGC [GA]CATG^[CT] RcaI 387 TCATGA T^CATGA SacI 746 GAGCTC GAGCT^C SapI 39 GCTCTTC GCTCTTC(1/4) SfaNI 90 GATGC GCATC(5/9) SspI 599 AATATT AAT^ATT XhoII 821 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 76 GAATTC [GA]^AATT[CT] 829 GAATTT AlwI 822 GATCC GGATC(4/5) 134 GGATC ApoI 76 GAATTC [GA]^AATT[CT] 829 GAATTT AvaII 444 GGTCC G^G[AT]CC 788 GGACC BcgI 128 GCAAAGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 491 GCATTGTTGTCG BpmI 235 CTGGAG CTGGAG(16/14) 727 CTGGAG BsrDI 264 CATTGC GCAATG(2/0) 482 GCAATG CfoI 408 GCGC GCG^C 753 GCGC DdeI 252 CTCAG C^T[ACGT]AG 433 CTCAG EcoT14I 96 CCAAGG C^C[AT][AT]GG 275 CCATGG HhaI 408 GCGC GCG^C 753 GCGC HinP1I 408 GCGC G^CGC 753 GCGC HinfI 378 GAATC G^A[ACGT]TC 863 GATTC PflMI 177 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 228 CCACTGCCTGG SfcI 211 CTACAG C^T[GA][CT]AG 452 CTGTAG StyI 96 CCAAGG C^C[AT][AT]GG 275 CCATGG TfiI 378 GAATC G^A[AT]TC 863 GATTC Tsp45I 115 GTCAC ^GT[GC]AC 187 GTGAC TspRI 229 CACTG CA[GC]TG[ACGT][ACGT]^ 605 CAGTG Van91I 177 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 228 CCACTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AluI BanII BbvI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I DpnII EarI EcoRII Fnu4HI HaeIII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp509I