(Linear) (Six-base) MAPSORT of: KIEE3667.seq  Check: 2564  from: 1  to: 495



KIEE3667.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:38  ..

AflIII A'CryG_T

Cuts at:      0     105     495 
   Size:        105     390
 
BanI G'GyrC_C

Cuts at:      0     302     495 
   Size:        302     193
 
BanII G_rGCy'C

Cuts at:      0     160     495 
   Size:        160     335
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     220     495 
   Size:        220     275
 
BglII A'GATC_T

Cuts at:      0     164     495 
   Size:        164     331
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     242     495 
   Size:        242     253
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     495 
   Size:        295     200
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     376     495 
   Size:        376     119
 
BsaWI w'CCGG_w

Cuts at:      0     110     495 
   Size:        110     385
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     298     495 
   Size:        271      27     197

  Fragments arranged by size: 

                 271     197      27
 
BseYI C'CCAG_C

Cuts at:      0     153     495 
   Size:        153     342
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154     495 
   Size:        154     341
 
Bsp1286I G_dGCh'C

Cuts at:      0     160     495 
   Size:        160     335
 
BsrDI GCAATG_nn'

Cuts at:      0     356     408     495 
   Size:        356      52      87

  Fragments arranged by size: 

                 356      87      52
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     333     495 
   Size:        333     162
 
BstYI r'GATC_y

Cuts at:      0     164     495 
   Size:        164     331
 
BtgI C'CryG_G

Cuts at:      0     175     495 
   Size:        175     320
 
EarI CTCTTCn'nnn_

Cuts at:      0     472     495 
   Size:        472      23
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     495 
   Size:        295     200
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     310     495 
   Size:        310     185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     477     495 
   Size:        477      18
 
NdeI CA'TA_TG

Cuts at:      0      78     495 
   Size:         78     417
 
NspI r_CATG'y

Cuts at:      0      34     109     479     495 
   Size:         34      75     370      16

  Fragments arranged by size: 

                 370      75      34      16
 
PciI A'CATG_T

Cuts at:      0     105     495 
   Size:        105     390
 
SexAI A'CCwGG_T

Cuts at:      0     140     495 
   Size:        140     355
 
StyI C'CwwG_G

Cuts at:      0     122     495 
   Size:        122     373
 

 Enzymes that do cut:

   AflIII     BanI    BanII     BbsI    BglII     BmrI     BpmI     BsaI
    BsaWI    BseRI    BseYI     BsgI Bsp1286I    BsrDI    BstXI    BstYI
     BtgI     EarI  Eco57MI    EcoNI     FalI     NdeI     NspI     PciI
    SexAI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI