Restriction map for
pFN21AE3917
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BbvCI BciVI BclI
BfaI BfrI BglI BlnI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DrdI DsaI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I KspI MaeI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI PacI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SmaI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 586 GTCTAC GT^[AC][GT]AC
AfaI 750 GTAC GT^AC
AgeI 414 ACCGGT A^CCGGT
ApaI 163 GGGCCC GGGCC^C
AspHI 465 GTGCTC G[AT]GC[AT]^C
AspI 655 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 566 CTCGAG C^[CT]CG[GA]G
BamHI 821 GGATCC G^GATCC
BbrPI 94 CACGTG CAC^GTG
BcgI 568 CGAGAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 539 AGATCT A^GATCT
BlpI 87 GCTGAGC GC^T[ACGT]AGC
BpmI 804 CTCCAG CTGGAG(16/14)
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
BsaBI 534 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 693 GGTCTC GGTCTC(1/5)
BsaWI 414 ACCGGT [AT]^CCGG[AT]
BseRI 265 GAGGAG GAGGAG(10/8)
BsgI 79 GTGCAG GTGCAG(16/14)
BsiHKAI 465 GTGCTC G[AT]GC[AT]^C
BsmBI 758 GAGACG CGTCTC(1/5)
BsmFI 42 GTCCC GGGAC(10/14)
BsoBI 566 CTCGAG C^[CT]CG[GA]G
BstAPI 510 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 457 TTCGAA TT^CGAA
CelII 87 GCTGAGC GC^T[ACGT]AGC
DraIII 168 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 315 CGGCCG [CT]^GGCC[GA]
EagI 315 CGGCCG C^GGCCG
EclXI 315 CGGCCG C^GGCCG
Eco52I 315 CGGCCG C^GGCCG
EcoT14I 110 CCTTGG C^C[AT][AT]GG
MamI 534 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 802 CACTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 30 GCCGGC GCC^GGC
NgoMIV 30 GCCGGC G^CCGGC
NlaIII 376 CATG CATG^
NspV 457 TTCGAA TT^CGAA
PaeR7I 566 CTCGAG C^TCGAG
PflFI 655 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 167 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 414 ACCGGT A^CCGGT
PmaCI 94 CACGTG CAC^GTG
PmlI 94 CACGTG CAC^GTG
PstI 181 CTGCAG CTGCA^G
PvuII 184 CAGCTG CAG^CTG
RsaI 750 GTAC GT^AC
SexAI 488 ACCTGGT A^CC[AT]GGT
SfcI 181 CTGCAG C^T[GA][CT]AG
SfuI 457 TTCGAA TT^CGAA
SgrAI 413 CACCGGTG C[GA]^CCGG[CT]G
SmlI 566 CTCGAG C^T[CT][GA]AG
SnaBI 733 TACGTA TAC^GTA
StyI 110 CCTTGG C^C[AT][AT]GG
Tth111I 655 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 167 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 566 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 4 GGCGCC G[GA]^CG[CT]C
787 GGCGCC
AlwI 822 GATCC GGATC(4/5)
821 GGATC
BanII 163 GGGCCC G[GA]GC[CT]^C
425 GGGCTC
BbeI 4 GGCGCC GGCGC^C
787 GGCGCC
BbsI 256 GAAGAC GAAGAC(2/6)
703 GTCTTC
BpuAI 256 GAAGAC GAAGAC(2/6)
703 GTCTTC
BsaAI 94 CACGTG [CT]AC^GT[GA]
733 TACGTA
BsaHI 4 GGCGCC G[GA]^CG[CT]C
787 GGCGCC
BsiEI 195 CGACCG CG[GA][CT]^CG
315 CGGCCG
BsmAI 758 GAGAC GTCTC(1/5)
694 GTCTC
BspMI 439 ACCTGC ACCTGC(4/8)
81 GCAGGT
BsrFI 30 GCCGGC [GA]^CCGG[CT]
414 ACCGGT
BstF5I 726 CATCC GGATG(2/0)
612 GGATG
BstYI 539 AGATCT [GA]^GATC[CT]
821 GGATCC
Cfr10I 30 GCCGGC [GA]^CCGG[CT]
414 ACCGGT
DpnII 540 GATC ^GATC
822 GATC
FokI 726 CATCC GGATG(9/13)
612 GGATG
HgaI 55 GACGC GACGC(5/10)
584 GCGTC
Hin1I 4 GGCGCC G[GA]^CG[CT]C
787 GGCGCC
KasI 4 GGCGCC G^GCGCC
787 GGCGCC
MboI 540 GATC ^GATC
822 GATC
MflI 539 AGATCT [GA]^GATC[CT]
821 GGATCC
MspA1I 184 CAGCTG C[AC]G^C[GT]G
274 CCGCTG
NarI 4 GGCGCC GG^CGCC
787 GGCGCC
NdeII 540 GATC ^GATC
822 GATC
Sau3AI 540 GATC ^GATC
822 GATC
SfaNI 122 GATGC GCATC(5/9)
725 GCATC
SfoI 4 GGCGCC GGC^GCC
787 GGCGCC
TaqI 458 TCGA T^CGA
567 TCGA
TthHB8I 458 TCGA T^CGA
567 TCGA
XhoII 539 AGATCT [GA]^GATC[CT]
821 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BanI BbvI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI Tsp45I TspRI