(Circular) (Six-base) MAPSORT of: pFN21AE3917.seq Check: 610 from: 1 to: 5539 pFN21AE3917 5539 bp With 182 enzymes: SgfI * March 28, 2012 11:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2077 2077 Size: 5539 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3349 278 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2592 2882 1937 Size: 655 290 4594 Fragments arranged by size: 4594 655 290 Acc65I G'GTAC_C Cuts at: 2860 2860 Size: 5539 AclI AA'CG_TT Cuts at: 1563 3666 4039 1563 Size: 2103 373 3063 Fragments arranged by size: 3063 2103 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2344 2654 3602 4986 5254 1632 Size: 712 310 948 1384 268 1917 Fragments arranged by size: 1917 1384 948 712 310 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5342 AflIII A'CryG_T Cuts at: 1203 4438 5415 1203 Size: 3235 977 1327 Fragments arranged by size: 3235 1327 977 AgeI A'CCGG_T Cuts at: 2419 4370 2419 Size: 1951 3588 AhdI GACnn_n'nnGTC Cuts at: 4268 4268 Size: 5539 AleI CACnn'nnGTG Cuts at: 1166 2811 1166 Size: 1645 3894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5539 AlwNI CAG_nnn'CTG Cuts at: 2092 2367 2521 3282 4854 2092 Size: 275 154 761 1572 2777 Fragments arranged by size: 2777 1572 761 275 154 ApaI G_GGCC'C Cuts at: 2172 2172 Size: 5539 ApaLI G'TGCA_C Cuts at: 3596 4752 3596 Size: 1156 4383 ApoI r'AATT_y Cuts at: 1493 2848 3074 3226 5335 1493 Size: 1355 226 152 2109 1697 Fragments arranged by size: 2109 1697 1355 226 152 AseI AT'TA_AT Cuts at: 160 1032 4093 160 Size: 872 3061 1606 Fragments arranged by size: 3061 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 AvaI C'yCGr_G Cuts at: 1870 1943 2571 2864 1870 Size: 73 628 293 4545 Fragments arranged by size: 4545 628 293 73 BamHI G'GATC_C Cuts at: 2826 2869 3236 2826 Size: 43 367 5129 Fragments arranged by size: 5129 367 43 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2422 2792 2860 4315 Size: 325 217 627 222 413 370 68 1455 Cuts at: 4315 618 Size: 1842 Fragments arranged by size: 1842 1455 627 413 370 325 222 217 68 BanII G_rGCy'C Cuts at: 1391 2172 2434 2858 1391 Size: 781 262 424 4072 Fragments arranged by size: 4072 781 424 262 BbeI G_GCGC'C Cuts at: 2013 2796 2013 Size: 783 4756 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2268 2701 928 Size: 971 369 433 3766 Fragments arranged by size: 3766 971 433 369 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2562 2596 3730 3764 1903 Size: 34 625 34 1134 34 3678 Fragments arranged by size: 3678 1134 625 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3431 4641 3431 Size: 1210 4329 BclI T'GATC_A Cuts at: 1548 1548 Size: 5539 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4150 136 Size: 107 122 71 631 605 2478 1525 Fragments arranged by size: 2478 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2544 5534 2544 Size: 2990 2549 BlpI GC'TnA_GC Cuts at: 2093 2956 2093 Size: 863 4676 Bme1580I G_kGCm'C Cuts at: 1790 2172 2787 3600 4756 1790 Size: 382 615 813 1156 2573 Fragments arranged by size: 2573 1156 813 615 382 BmgBI CAC'GTC Cuts at: 1318 2707 2767 1318 Size: 1389 60 4090 Fragments arranged by size: 4090 1389 60 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 3270 4228 Size: 416 53 83 399 24 712 1141 958 Cuts at: 4228 442 Size: 1753 Fragments arranged by size: 1753 1141 958 712 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2794 4199 1239 Size: 1555 1405 2579 Fragments arranged by size: 2579 1555 1405 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3664 4529 4827 3664 Size: 865 298 4376 Fragments arranged by size: 4376 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2704 4202 882 Size: 617 1205 1498 2219 Fragments arranged by size: 2219 1498 1205 617 BsaAI yAC'GTr Cuts at: 493 1204 2101 2740 3330 493 Size: 711 897 639 590 2702 Fragments arranged by size: 2702 897 711 639 590 BsaBI GATnn'nnATC Cuts at: 2543 5533 2543 Size: 2990 2549 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2793 3346 3728 Size: 53 83 186 936 477 783 553 382 Cuts at: 3728 4363 275 Size: 635 1451 Fragments arranged by size: 1451 936 783 635 553 477 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2419 3972 4370 4644 4791 5202 2419 Size: 1553 398 274 147 411 2756 Fragments arranged by size: 2756 1553 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5314 Fragments arranged by size: 5314 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2285 1186 Size: 1099 4440 BseYI C'CCAG_C Cuts at: 2252 2367 2394 4742 5238 2252 Size: 115 27 2348 496 2553 Fragments arranged by size: 2553 2348 496 115 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2105 1163 Size: 573 369 4597 Fragments arranged by size: 4597 573 369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2203 2323 2989 3750 4778 1540 Size: 275 186 202 120 666 761 1028 2301 Fragments arranged by size: 2301 1028 761 666 275 202 186 120 BsiHKAI G_wGCw'C Cuts at: 2474 3600 3685 4756 2474 Size: 1126 85 1071 3257 Fragments arranged by size: 3257 1126 1071 85 BsmI GAATG_Cn' Cuts at: 3050 3143 3050 Size: 93 5446 BsmBI CGTCTCn'nnnn_ Cuts at: 2757 2757 Size: 5539 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2434 2474 2787 2858 3600 3685 Size: 399 382 262 40 313 71 742 85 Cuts at: 3685 4756 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 313 262 85 71 40 BspEI T'CCGG_A Cuts at: 5202 5202 Size: 5539 BspHI T'CATG_A Cuts at: 3428 5158 3428 Size: 1730 3809 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2077 2453 2894 844 Size: 1233 376 441 3489 Fragments arranged by size: 3489 1233 441 376 BsrBI CCG'CTC Cuts at: 1394 1823 3426 1394 Size: 429 1603 3507 Fragments arranged by size: 3507 1603 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4034 4208 65 Size: 1183 2786 174 1396 Fragments arranged by size: 2786 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 2419 4183 4370 1700 Size: 110 225 384 1764 187 2869 Fragments arranged by size: 2869 1764 384 225 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5539 BssHII G'CGCG_C Cuts at: 1925 2294 2308 2310 1925 Size: 369 14 2 5154 Fragments arranged by size: 5154 369 14 2 BssSI C'ACGA_G Cuts at: 3599 4611 3599 Size: 1012 4527 BstAPI GCAn_nnn'nTGC Cuts at: 794 2521 794 Size: 1727 3812 BstBI TT'CG_AA Cuts at: 2463 4343 2463 Size: 1880 3659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5539 BstYI r'GATC_y Cuts at: 1972 2544 2826 2869 3236 3621 3638 5090 5176 Size: 572 282 43 367 385 17 1452 86 Cuts at: 5176 5188 5534 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 572 385 367 346 282 86 43 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4048 Fragments arranged by size: 4048 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2140 487 Size: 683 56 914 3886 Fragments arranged by size: 3886 914 683 56 BtsI GCAGTG_nn' Cuts at: 2238 3055 3849 3869 2238 Size: 817 794 20 3908 Fragments arranged by size: 3908 817 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5539 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2097 2132 529 Size: 35 1533 35 3936 Fragments arranged by size: 3936 1533 35 35 DraI TTT'AAA Cuts at: 2843 2843 Size: 5539 DraIII CAC_nnn'GTG Cuts at: 2178 2178 Size: 5539 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4546 809 Size: 507 3230 1802 Fragments arranged by size: 3230 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2320 2986 3875 Size: 54 1425 50 275 18 490 666 889 Cuts at: 3875 5504 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 666 490 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2320 2986 1537 Size: 275 508 666 4090 Fragments arranged by size: 4090 666 508 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3469 1353 Size: 2116 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2054 2597 4126 4500 4646 387 Size: 1064 138 465 543 1529 374 146 1280 Fragments arranged by size: 1529 1280 1064 543 465 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2344 2654 2794 3602 4199 4986 5254 Size: 393 712 310 140 808 597 787 268 Cuts at: 5254 1239 Size: 1524 Fragments arranged by size: 1524 808 787 712 597 393 310 268 140 EcoRI G'AATT_C Cuts at: 2848 3226 2848 Size: 378 5161 FspI TGC'GCA Cuts at: 4045 4045 Size: 5539 HaeII r_GCGC'y Cuts at: 1678 2013 2211 2535 2571 2796 4686 1678 Size: 335 198 324 36 225 1890 2531 Fragments arranged by size: 2531 1890 335 324 225 198 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4180 4212 4254 4286 1373 Size: 32 135 32 2608 32 42 32 2626 Fragments arranged by size: 2626 2608 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2883 3129 669 Size: 988 281 945 246 3079 Fragments arranged by size: 3079 988 945 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5539 HpaI GTT'AAC Cuts at: 3129 3129 Size: 5539 KasI G'GCGC_C Cuts at: 2009 2792 2009 Size: 783 4756 KpnI G_GTAC'C Cuts at: 2864 2864 Size: 5539 MfeI C'AATT_G Cuts at: 3138 3138 Size: 5539 MluI A'CGCG_T Cuts at: 5415 5415 Size: 5539 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4653 4837 1305 Size: 3348 184 2007 Fragments arranged by size: 3348 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3717 Fragments arranged by size: 3717 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2811 3497 3856 4015 518 Size: 648 1645 686 359 159 2042 Fragments arranged by size: 2042 1645 686 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2191 2281 2955 3632 4780 5025 1606 Size: 585 90 674 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 674 585 245 90 NaeI GCC'GGC Cuts at: 2037 2037 Size: 5539 NarI GG'CG_CC Cuts at: 2010 2793 2010 Size: 783 4756 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4048 Fragments arranged by size: 4048 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5539 NgoMIV G'CCGG_C Cuts at: 2035 2035 Size: 5539 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5539 NotI GC'GGCC_GC Cuts at: 2986 2986 Size: 5539 NspI r_CATG'y Cuts at: 1132 1229 2897 4442 1132 Size: 97 1668 1545 2229 Fragments arranged by size: 2229 1668 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5539 PciI A'CATG_T Cuts at: 4438 4438 Size: 5539 PflMI CCAn_nnn'nTGG Cuts at: 1108 2178 1108 Size: 1070 4469 PmeI GTTT'AAAC Cuts at: 2843 2843 Size: 5539 PmlI CAC'GTG Cuts at: 2101 2101 Size: 5539 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3608 3640 5121 5153 3608 Size: 32 1481 32 3994 Fragments arranged by size: 3994 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5539 PsiI TTA'TAA Cuts at: 3109 3109 Size: 5539 PspOMI G'GGCC_C Cuts at: 2168 2168 Size: 5539 PstI C_TGCA'G Cuts at: 830 2190 2891 830 Size: 1360 701 3478 Fragments arranged by size: 3478 1360 701 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5539 PvuII CAG'CTG Cuts at: 2191 2191 Size: 5539 SalI G'TCGA_C Cuts at: 1936 2881 1936 Size: 945 4594 SbfI CC_TGCA'GG Cuts at: 2891 2891 Size: 5539 ScaI AGT'ACT Cuts at: 3787 5499 3787 Size: 1712 3827 SexAI A'CCwGG_T Cuts at: 2493 2493 Size: 5539 SfcI C'TryA_G Cuts at: 826 1045 2186 2887 4022 4703 4894 826 Size: 219 1141 701 1135 681 191 1471 Fragments arranged by size: 1471 1141 1135 701 681 219 191 SfoI GGC'GCC Cuts at: 2011 2794 2011 Size: 783 4756 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5539 SgrAI Cr'CCGG_yG Cuts at: 1700 2419 1700 Size: 719 4820 SmaI CCC'GGG Cuts at: 1872 2866 1872 Size: 994 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2571 3643 4544 4806 820 Size: 197 926 628 1072 901 262 1553 Fragments arranged by size: 1553 1072 926 901 628 262 197 SnaBI TAC'GTA Cuts at: 493 2740 3330 493 Size: 2247 590 2702 Fragments arranged by size: 2702 2247 590 SpeI A'CTAG_T Cuts at: 152 152 Size: 5539 SphI G_CATG'C Cuts at: 1132 2897 1132 Size: 1765 3774 SspI AAT'ATT Cuts at: 5 52 3463 5 Size: 47 3411 2081 Fragments arranged by size: 3411 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5539 StyI C'CwwG_G Cuts at: 513 1065 2004 2115 2978 513 Size: 552 939 111 863 3074 Fragments arranged by size: 3074 939 863 552 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2767 3566 3583 3736 3921 4366 325 Size: 1255 1187 799 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1187 799 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3785 5497 96 Size: 274 80 33 51 1443 1808 1712 138 Fragments arranged by size: 1808 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2495 2527 5499 5531 2495 Size: 32 2972 32 2503 Fragments arranged by size: 2972 2503 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2663 1316 Size: 1347 4192 XbaI T'CTAG_A Cuts at: 2875 2875 Size: 5539 XhoI C'TCGA_G Cuts at: 1943 2571 1943 Size: 628 4911 XmaI C'CCGG_G Cuts at: 1870 2864 1870 Size: 994 4545 XmnI GAAnn'nnTTC Cuts at: 3668 3668 Size: 5539 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3347 276 Size: 53 83 186 936 1813 2468 Fragments arranged by size: 2468 1813 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI