################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccIII AcsI AflII AgeI AlwI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspEI BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI DraIII EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SalI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1535 GGTACC G^GTACC AclI 1612 AACGTT AA^CGTT Alw44I 1114 GTGCAC G^TGCAC AlwNI 1774 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1766 AGCGCT AGC^GCT ApaLI 1114 GTGCAC G^TGCAC Asp718I 1535 GGTACC G^GTACC AvaI 73 CCCGGG C^[CT]CG[GA]G BbeI 850 GGCGCC GGCGC^C BlpI 1762 GCTGAGC GC^T[ACGT]AGC BpmI 673 CTGGAG CTGGAG(16/14) Bpu1102I 1762 GCTGAGC GC^T[ACGT]AGC BsgI 1720 CTGCAC GTGCAG(16/14) BsiEI 132 CGGCCG CG[GA][CT]^CG BsoBI 73 CCCGGG C^[CT]CG[GA]G BspDI 1369 ATCGAT AT^CGAT BspHI 344 TCATGA T^CATGA BspLU11I 511 ACATGT A^CATGT BsrDI 1518 CATTGC GCAATG(2/0) BstAPI 1107 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 702 GGTGACC G^GT[ACGT]ACC BstPI 702 GGTGACC G^GT[ACGT]ACC CelII 1762 GCTGAGC GC^T[ACGT]AGC ClaI 1369 ATCGAT AT^CGAT EagI 132 CGGCCG C^GGCCG EarI 9 GAAGAG CTCTTC(1/4) EclXI 132 CGGCCG C^GGCCG Eco47III 1766 AGCGCT AGC^GCT Eco52I 132 CGGCCG C^GGCCG EcoO65I 702 GGTGACC G^GT[ACGT]ACC KasI 850 GGCGCC G^GCGCC KpnI 1535 GGTACC GGTAC^C Ksp632I 9 GAAGAG CTCTTC(1/4) KspI 1168 CCGCGG CCGC^GG NaeI 352 GCCGGC GCC^GGC NarI 850 GGCGCC GG^CGCC NgoMIV 352 GCCGGC G^CCGGC NotI 131 GCGGCCGC GC^GGCCGC NspI 511 ACATGT [GA]CATG^[CT] PleI 1135 GAGTC GAGTC(4/5) Psp1406I 1612 AACGTT AA^CGTT PstI 1100 CTGCAG CTGCA^G PvuII 1411 CAGCTG CAG^CTG RcaI 344 TCATGA T^CATGA SacI 1820 GAGCTC GAGCT^C SacII 1168 CCGCGG CCGC^GG SapI 9 GAAGAGC GCTCTTC(1/4) SexAI 473 ACCTGGT A^CC[AT]GGT SfiI 106 GGCCCCGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 850 GGCGCC GGC^GCC SmaI 73 CCCGGG CCC^GGG TfiI 305 GAATC G^A[AT]TC XmaCI 73 CCCGGG C^CCGGG XmaI 73 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 736 GTCTAC GT^[AC][GT]AC 1636 GTCTAC AcyI 850 GGCGCC G[GA]^CG[CT]C 1018 GACGCC AhdI 613 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 705 GACCCTTTGTC AocI 907 CCTCAGG CC^T[ACGT]AGG 1740 CCTCAGG AspEI 613 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 705 GACCCTTTGTC AspHI 1114 GTGCAC G[AT]GC[AT]^C 1820 GAGCTC BalI 516 TGGCCA TGG^CCA 584 TGGCCA BcgI 1874 GCAGCAAGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1877 GCAAGGTCGTCG BglI 107 GCCCCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 577 GCCAGCCTGGC BsaAI 805 TACGTG [CT]AC^GT[GA] 1651 TACGTG BsaHI 850 GGCGCC G[GA]^CG[CT]C 1018 GACGCC BsaI 157 GAGACC GGTCTC(1/5) 1240 GGTCTC BsiHKAI 1114 GTGCAC G[AT]GC[AT]^C 1820 GAGCTC BsmAI 157 GAGAC GTCTC(1/5) 1241 GTCTC BstXI 953 CCATTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1022 CCATCATCCTGG Bsu36I 907 CCTCAGG CC^T[ACGT]AGG 1740 CCTCAGG DrdI 1828 GACGCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1857 GACCCCAGTGTC Eam1105I 613 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 705 GACCCTTTGTC Eco81I 907 CCTCAGG CC^T[ACGT]AGG 1740 CCTCAGG Hin1I 850 GGCGCC G[GA]^CG[CT]C 1018 GACGCC HinfI 305 GAATC G^A[ACGT]TC 1135 GAGTC MluNI 516 TGGCCA TGG^CCA 584 TGGCCA MscI 516 TGGCCA TGG^CCA 584 TGGCCA PflMI 1676 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1803 CCACCACTTGG PpuMI 901 GGGTCCC [GA]G^G[AT]CC[CT] 928 GGGACCC SfcI 30 CTATAG C^T[GA][CT]AG 1100 CTGCAG SmlI 654 CTTGAG C^T[CT][GA]AG 877 CTCAAG StuI 393 AGGCCT AGG^CCT 1457 AGGCCT Van91I 1676 CCATGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1803 CCACCACTTGG XcmI 572 CCAATGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1748 CCAAGGGCACCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AfaI AflIII AluI ApaI AvaII BanI BanII BbsI BbvI BcnI BglII BmyI BpuAI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII