(Circular) (Six-base) MAPSORT of: pFN21AE4545.seq Check: 8266 from: 1 to: 6610 pFN21AE4545 6610 bp With 182 enzymes: SgfI * March 28, 2012 14:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4420 278 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2742 3642 3953 1937 Size: 805 900 311 4594 Fragments arranged by size: 4594 900 805 311 Acc65I G'GTAC_C Cuts at: 3540 3931 3540 Size: 391 6219 AclI AA'CG_TT Cuts at: 1563 3618 4737 5110 1563 Size: 2055 1119 373 3063 Fragments arranged by size: 3063 2055 1119 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4673 6057 6325 1632 Size: 3041 1384 268 1917 Fragments arranged by size: 3041 1917 1384 268 AfeI AGC'GCT Cuts at: 3773 3773 Size: 6610 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6413 AflIII A'CryG_T Cuts at: 1203 2226 2516 3657 5509 6486 1203 Size: 1023 290 1141 1852 977 1327 Fragments arranged by size: 1852 1327 1141 1023 977 290 AgeI A'CCGG_T Cuts at: 5441 5441 Size: 6610 AhdI GACnn_n'nnGTC Cuts at: 2623 2715 5339 2623 Size: 92 2624 3894 Fragments arranged by size: 3894 2624 92 AleI CACnn'nnGTG Cuts at: 1166 3656 1166 Size: 2490 4120 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3467 1184 Size: 2283 4327 AlwNI CAG_nnn'CTG Cuts at: 3784 4353 5925 3784 Size: 569 1572 4469 Fragments arranged by size: 4469 1572 569 ApaI G_GGCC'C Cuts at: 2066 2085 2095 2695 2974 2066 Size: 19 10 600 279 5702 Fragments arranged by size: 5702 600 279 19 10 ApaLI G'TGCA_C Cuts at: 3119 4667 5823 3119 Size: 1548 1156 3906 Fragments arranged by size: 3906 1548 1156 ApoI r'AATT_y Cuts at: 1493 3919 4145 4297 6406 1493 Size: 2426 226 152 2109 1697 Fragments arranged by size: 2426 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5164 160 Size: 872 4132 1606 Fragments arranged by size: 4132 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 AvaI C'yCGr_G Cuts at: 1870 1943 2078 3935 1870 Size: 73 135 1857 4545 Fragments arranged by size: 4545 1857 135 73 BamHI G'GATC_C Cuts at: 3940 4307 3940 Size: 367 6243 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 2855 3443 3540 3758 Size: 325 217 627 896 172 588 97 218 Cuts at: 3758 3931 5386 618 Size: 173 1455 1842 Fragments arranged by size: 1842 1455 896 627 588 325 218 217 173 172 97 BanII G_rGCy'C Cuts at: 1391 2066 2085 2095 2695 2974 3829 3929 1391 Size: 675 19 10 600 279 855 100 4072 Fragments arranged by size: 4072 855 675 600 279 100 19 10 BbeI G_GCGC'C Cuts at: 2859 2859 Size: 6610 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2419 2574 3073 3403 928 Size: 971 520 155 499 330 4135 Fragments arranged by size: 4135 971 520 499 330 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3871 3902 3905 4801 4835 1903 Size: 34 1931 3 31 3 896 34 3678 Fragments arranged by size: 3678 1931 896 34 34 31 3 3 BciVI GTATCCnnnnn_n' Cuts at: 4502 5712 4502 Size: 1210 5400 BclI T'GATC_A Cuts at: 1548 1548 Size: 6610 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2118 2588 5221 Size: 107 122 71 631 605 446 470 2633 Cuts at: 5221 136 Size: 1525 Fragments arranged by size: 2633 1525 631 605 470 446 122 107 71 BglII A'GATC_T Cuts at: 2331 2553 3336 6605 2331 Size: 222 783 3269 2336 Fragments arranged by size: 3269 2336 783 222 BlpI GC'TnA_GC Cuts at: 3768 4027 3768 Size: 259 6351 Bme1580I G_kGCm'C Cuts at: 1790 2066 2085 2095 2695 2974 3123 3361 3761 Size: 276 19 10 600 279 149 238 400 Cuts at: 3761 4671 5827 1790 Size: 910 1156 2573 Fragments arranged by size: 2573 1156 910 600 400 279 276 238 149 19 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6610 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3860 4341 Size: 416 53 83 399 24 1293 1150 481 Cuts at: 4341 5299 442 Size: 958 1753 Fragments arranged by size: 1753 1293 1150 958 481 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6610 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2699 5270 1239 Size: 1460 2571 2579 Fragments arranged by size: 2579 2571 1460 Bpu10I CC'TnA_GC Cuts at: 3828 3828 Size: 6610 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2680 2867 4735 5600 5898 2680 Size: 187 1868 865 298 3392 Fragments arranged by size: 3392 1868 865 298 187 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 3251 5273 882 Size: 617 657 1095 2022 2219 Fragments arranged by size: 2219 2022 1095 657 617 BsaAI yAC'GTr Cuts at: 493 1204 2812 3658 4401 493 Size: 711 1608 846 743 2702 Fragments arranged by size: 2702 1608 846 743 711 BsaBI GATnn'nnATC Cuts at: 6604 6604 Size: 6610 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2856 3024 4417 4799 Size: 53 83 186 936 1323 168 1393 382 Cuts at: 4799 5434 275 Size: 635 1451 Fragments arranged by size: 1451 1393 1323 936 635 382 186 168 83 53 BsaWI w'CCGG_w Cuts at: 5043 5441 5715 5862 6273 5043 Size: 398 274 147 411 5380 Fragments arranged by size: 5380 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6385 Fragments arranged by size: 6385 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3281 3290 3734 1186 Size: 2095 9 444 4062 Fragments arranged by size: 4062 2095 444 9 BseYI C'CCAG_C Cuts at: 2338 2730 2902 3687 3789 5813 6309 2338 Size: 392 172 785 102 2024 496 2639 Fragments arranged by size: 2639 2024 785 496 392 172 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3710 1163 Size: 573 1974 4063 Fragments arranged by size: 4063 1974 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2140 4060 4821 5849 1540 Size: 275 186 139 1920 761 1028 2301 Fragments arranged by size: 2301 1920 1028 761 275 186 139 BsiHKAI G_wGCw'C Cuts at: 3123 3829 4671 4756 5827 3123 Size: 706 842 85 1071 3906 Fragments arranged by size: 3906 1071 842 706 85 BsmI GAATG_Cn' Cuts at: 4121 4214 4121 Size: 93 6517 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2085 2095 2695 2974 3123 3361 Size: 399 276 19 10 600 279 149 238 Cuts at: 3361 3761 3829 3929 4671 4756 5827 1391 Size: 400 68 100 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 400 399 279 276 238 149 100 85 68 19 10 BspEI T'CCGG_A Cuts at: 6273 6273 Size: 6610 BspHI T'CATG_A Cuts at: 2349 4499 6229 2349 Size: 2150 1730 2730 Fragments arranged by size: 2730 2150 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3965 844 Size: 3121 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4497 1394 Size: 429 2674 3507 Fragments arranged by size: 3507 2674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3522 5105 5279 65 Size: 1183 2274 1583 174 1396 Fragments arranged by size: 2274 1583 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2357 2622 3284 5254 5441 1700 Size: 110 547 265 662 1970 187 2869 Fragments arranged by size: 2869 1970 662 547 265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6610 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6610 BssSI C'ACGA_G Cuts at: 4670 5682 4670 Size: 1012 5598 BstAPI GCAn_nnn'nTGC Cuts at: 794 3118 794 Size: 2324 4286 BstBI TT'CG_AA Cuts at: 5414 5414 Size: 6610 BstEII G'GTnAC_C Cuts at: 1184 2707 1184 Size: 1523 5087 BstXI CCAn_nnnn'nTGG Cuts at: 2965 3034 2965 Size: 69 6541 BstYI r'GATC_y Cuts at: 1972 2331 2553 3336 3940 4307 4692 4709 6161 Size: 359 222 783 604 367 385 17 1452 Cuts at: 6161 6247 6259 6605 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 783 604 385 367 359 346 222 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2913 3746 2913 Size: 833 5777 BtgI C'CryG_G Cuts at: 513 1065 2004 2115 2211 2415 2772 3173 3384 Size: 552 939 111 96 204 357 401 211 Cuts at: 3384 3665 3681 3762 513 Size: 281 16 81 3361 Fragments arranged by size: 3361 939 552 401 357 281 211 204 111 96 81 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2056 3148 487 Size: 683 56 830 1092 3949 Fragments arranged by size: 3949 1092 830 683 56 BtsI GCAGTG_nn' Cuts at: 2793 4126 4920 4940 2793 Size: 1333 794 20 4463 Fragments arranged by size: 4463 1333 794 20 ClaI AT'CG_AT Cuts at: 1554 3375 1554 Size: 1821 4789 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6575 DraI TTT'AAA Cuts at: 3914 3914 Size: 6610 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3839 3868 5617 809 Size: 507 2523 29 1749 1802 Fragments arranged by size: 2523 1802 1749 507 29 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2097 2137 2145 Size: 54 1425 50 275 18 267 40 8 Cuts at: 2145 2521 2589 3435 4057 4946 6575 8 Size: 376 68 846 622 889 1629 43 Fragments arranged by size: 1629 1425 889 846 622 376 275 267 68 54 50 43 40 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2137 4057 1537 Size: 275 325 1920 4090 Fragments arranged by size: 4090 1920 325 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2009 4540 1353 Size: 656 2531 3423 Fragments arranged by size: 3423 2531 656 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2277 2549 5197 5571 5717 387 Size: 1064 138 688 272 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 688 374 272 146 138 EcoICRI GAG'CTC Cuts at: 3827 3827 Size: 6610 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2699 4673 5270 6057 6325 1239 Size: 393 1067 1974 597 787 268 1524 Fragments arranged by size: 1974 1524 1067 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2062 2063 2091 2092 2691 2692 2907 2934 2970 Size: 1 28 1 599 1 215 27 36 Cuts at: 2970 2971 3802 3847 2062 Size: 1 831 45 4825 Fragments arranged by size: 4825 831 599 215 45 36 28 27 1 1 1 1 EcoRI G'AATT_C Cuts at: 3919 4297 3919 Size: 378 6232 FspI TGC'GCA Cuts at: 5116 5116 Size: 6610 HaeII r_GCGC'y Cuts at: 1678 2022 2236 2859 3775 5757 1678 Size: 344 214 623 916 1982 2531 Fragments arranged by size: 2531 1982 916 623 344 214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 2701 2733 3416 Size: 32 135 32 1037 32 60 32 683 Cuts at: 3416 3448 5251 5283 5325 5357 1373 Size: 32 1803 32 42 32 2626 Fragments arranged by size: 2626 1803 1037 683 135 60 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3954 4200 669 Size: 988 281 2016 246 3079 Fragments arranged by size: 3079 2016 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6610 HpaI GTT'AAC Cuts at: 4200 4200 Size: 6610 KasI G'GCGC_C Cuts at: 2855 2855 Size: 6610 KpnI G_GTAC'C Cuts at: 3544 3935 3544 Size: 391 6219 MfeI C'AATT_G Cuts at: 4209 4209 Size: 6610 MluI A'CGCG_T Cuts at: 6486 6486 Size: 6610 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2284 5724 5908 1305 Size: 979 3440 184 2007 Fragments arranged by size: 3440 2007 979 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2523 2591 10 Size: 54 1425 343 691 68 4029 Fragments arranged by size: 4029 1425 691 343 68 54 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3188 3656 4568 4927 5086 518 Size: 648 1287 735 468 912 359 159 2042 Fragments arranged by size: 2042 1287 912 735 648 468 359 159 MspA1I CmG'CkG Cuts at: 1606 3175 3418 3441 3589 3637 3724 4026 4703 Size: 1569 243 23 148 48 87 302 677 Cuts at: 4703 5851 6096 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1569 1148 677 302 245 243 148 87 48 23 NaeI GCC'GGC Cuts at: 2359 2359 Size: 6610 NarI GG'CG_CC Cuts at: 2856 2856 Size: 6610 NcoI C'CATG_G Cuts at: 513 1065 2004 2415 3384 3681 3762 513 Size: 552 939 411 969 297 81 3361 Fragments arranged by size: 3361 969 939 552 411 297 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 6610 NgoMIV G'CCGG_C Cuts at: 2357 2357 Size: 6610 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6610 NotI GC'GGCC_GC Cuts at: 2137 4057 2137 Size: 1920 4690 NspI r_CATG'y Cuts at: 1132 1229 2520 3968 5513 1132 Size: 97 1291 1448 1545 2229 Fragments arranged by size: 2229 1545 1448 1291 97 PasI CC'CwG_GG Cuts at: 1348 2508 2983 1348 Size: 1160 475 4975 Fragments arranged by size: 4975 1160 475 PciI A'CATG_T Cuts at: 2516 5509 2516 Size: 2993 3617 PflMI CCAn_nnn'nTGG Cuts at: 1108 3687 3814 1108 Size: 2579 127 3904 Fragments arranged by size: 3904 2579 127 PmeI GTTT'AAAC Cuts at: 3914 3914 Size: 6610 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4679 4711 6192 6224 4679 Size: 32 1481 32 5065 Fragments arranged by size: 5065 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2907 2934 2907 Size: 27 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6610 PsiI TTA'TAA Cuts at: 4180 4180 Size: 6610 PspOMI G'GGCC_C Cuts at: 2062 2081 2091 2691 2970 2062 Size: 19 10 600 279 5702 Fragments arranged by size: 5702 600 279 19 10 PstI C_TGCA'G Cuts at: 830 3109 3962 830 Size: 2279 853 3478 Fragments arranged by size: 3478 2279 853 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6610 PvuII CAG'CTG Cuts at: 3418 3418 Size: 6610 SacI G_AGCT'C Cuts at: 3829 3829 Size: 6610 SacII CC_GC'GG Cuts at: 3176 3176 Size: 6610 SalI G'TCGA_C Cuts at: 1936 3952 1936 Size: 2016 4594 SanDI GG'GwC_CC Cuts at: 2907 2934 2907 Size: 27 6583 SapI GCTCTTCn'nnn_ Cuts at: 2009 2009 Size: 6610 SbfI CC_TGCA'GG Cuts at: 3962 3962 Size: 6610 ScaI AGT'ACT Cuts at: 4858 6570 4858 Size: 1712 4898 SexAI A'CCwGG_T Cuts at: 2478 2478 Size: 6610 SfcI C'TryA_G Cuts at: 826 1045 2035 3105 3958 5093 5774 5965 826 Size: 219 990 1070 853 1135 681 191 1471 Fragments arranged by size: 1471 1135 1070 990 853 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2118 2118 Size: 6610 SfoI GGC'GCC Cuts at: 2857 2857 Size: 6610 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6610 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6610 SmaI CCC'GGG Cuts at: 1872 2080 3937 1872 Size: 208 1857 4545 Fragments arranged by size: 4545 1857 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 2659 2882 4714 5615 5877 820 Size: 197 926 716 223 1832 901 262 1553 Fragments arranged by size: 1832 1553 926 901 716 262 223 197 SnaBI TAC'GTA Cuts at: 493 4401 493 Size: 3908 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6610 SphI G_CATG'C Cuts at: 1132 3968 1132 Size: 2836 3774 SrfI GCCC'GGGC Cuts at: 2080 2080 Size: 6610 SspI AAT'ATT Cuts at: 5 52 4534 5 Size: 47 4482 2081 Fragments arranged by size: 4482 2081 47 StuI AGG'CCT Cuts at: 1517 2400 3464 1517 Size: 883 1064 4663 Fragments arranged by size: 4663 1064 883 StyI C'CwwG_G Cuts at: 513 1065 2004 2100 2415 3129 3384 3681 3753 Size: 552 939 96 315 714 255 297 72 Cuts at: 3753 3762 4049 513 Size: 9 287 3074 Fragments arranged by size: 3074 939 714 552 315 297 287 255 96 72 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2722 3481 4637 4654 4807 4992 5437 Size: 1255 1142 759 1156 17 153 185 445 Cuts at: 5437 325 Size: 1498 Fragments arranged by size: 1498 1255 1156 1142 759 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2787 3624 4856 Size: 274 80 33 51 1443 810 837 1232 Cuts at: 4856 6568 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1232 837 810 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2667 2699 2769 2801 6570 6602 2667 Size: 32 70 32 3769 32 2675 Fragments arranged by size: 3769 2675 70 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6610 XbaI T'CTAG_A Cuts at: 3946 3946 Size: 6610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2584 3760 2584 Size: 1176 5434 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6610 XmaI C'CCGG_G Cuts at: 1870 2078 3935 1870 Size: 208 1857 4545 Fragments arranged by size: 4545 1857 208 XmnI GAAnn'nnTTC Cuts at: 4739 4739 Size: 6610 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4418 276 Size: 53 83 186 936 2884 2468 Fragments arranged by size: 2884 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SwaI