(Linear) (Six-base) MAPSORT of: KIEE4545.seq Check: 6453 from: 1 to: 1905 KIEE4545.orf With 182 enzymes: SgfI * March 22, 2012 18:19 .. AccI GT'mk_AC Cuts at: 0 737 1637 1905 Size: 737 900 268 Fragments arranged by size: 900 737 268 Acc65I G'GTAC_C Cuts at: 0 1535 1905 Size: 1535 370 AclI AA'CG_TT Cuts at: 0 1613 1905 Size: 1613 292 AfeI AGC'GCT Cuts at: 0 1768 1905 Size: 1768 137 AflIII A'CryG_T Cuts at: 0 221 511 1652 1905 Size: 221 290 1141 253 Fragments arranged by size: 1141 290 253 221 AhdI GACnn_n'nnGTC Cuts at: 0 618 710 1905 Size: 618 92 1195 Fragments arranged by size: 1195 618 92 AleI CACnn'nnGTG Cuts at: 0 1651 1905 Size: 1651 254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1462 1905 Size: 1462 443 AlwNI CAG_nnn'CTG Cuts at: 0 1779 1905 Size: 1779 126 ApaI G_GGCC'C Cuts at: 0 61 80 90 690 969 1905 Size: 61 19 10 600 279 936 Fragments arranged by size: 936 600 279 61 19 10 ApaLI G'TGCA_C Cuts at: 0 1114 1905 Size: 1114 791 AvaI C'yCGr_G Cuts at: 0 73 1905 Size: 73 1832 BanI G'GyrC_C Cuts at: 0 678 850 1438 1535 1753 1905 Size: 678 172 588 97 218 152 Fragments arranged by size: 678 588 218 172 152 97 BanII G_rGCy'C Cuts at: 0 61 80 90 690 969 1824 1905 Size: 61 19 10 600 279 855 81 Fragments arranged by size: 855 600 279 81 61 19 10 BbeI G_GCGC'C Cuts at: 0 854 1905 Size: 854 1051 BbsI GAAGACnn'nnnn_ Cuts at: 0 414 569 1068 1398 1905 Size: 414 155 499 330 507 Fragments arranged by size: 507 499 414 330 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1863 1866 1897 1900 1905 Size: 1863 3 31 3 5 Fragments arranged by size: 1863 31 5 3 3 BglI GCCn_nnn'nGGC Cuts at: 0 113 583 1905 Size: 113 470 1322 Fragments arranged by size: 1322 470 113 BglII A'GATC_T Cuts at: 0 326 548 1331 1905 Size: 326 222 783 574 Fragments arranged by size: 783 574 326 222 BlpI GC'TnA_GC Cuts at: 0 1763 1905 Size: 1763 142 Bme1580I G_kGCm'C Cuts at: 0 61 80 90 690 969 1118 1356 1756 Size: 61 19 10 600 279 149 238 400 Cuts at: 1756 1905 Size: 149 Fragments arranged by size: 600 400 279 238 149 149 61 19 10 BmrI ACTGGGnnnn_n' Cuts at: 0 705 1855 1905 Size: 705 1150 50 Fragments arranged by size: 1150 705 50 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 1905 Size: 694 1211 Bpu10I CC'TnA_GC Cuts at: 0 1823 1905 Size: 1823 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 675 862 1905 Size: 675 187 1043 Fragments arranged by size: 1043 675 187 BsaI GGTCTCn'nnnn_ Cuts at: 0 151 1246 1905 Size: 151 1095 659 Fragments arranged by size: 1095 659 151 BsaAI yAC'GTr Cuts at: 0 807 1653 1905 Size: 807 846 252 Fragments arranged by size: 846 807 252 BsaHI Gr'CG_yC Cuts at: 0 851 1019 1905 Size: 851 168 886 Fragments arranged by size: 886 851 168 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1276 1285 1729 1905 Size: 1276 9 444 176 Fragments arranged by size: 1276 444 176 9 BseYI C'CCAG_C Cuts at: 0 333 725 897 1682 1784 1905 Size: 333 392 172 785 102 121 Fragments arranged by size: 785 392 333 172 121 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1705 1905 Size: 1705 200 BsiEI CG_ry'CG Cuts at: 0 135 1905 Size: 135 1770 BsiHKAI G_wGCw'C Cuts at: 0 1118 1824 1905 Size: 1118 706 81 Fragments arranged by size: 1118 706 81 Bsp1286I G_dGCh'C Cuts at: 0 61 80 90 690 969 1118 1356 1756 Size: 61 19 10 600 279 149 238 400 Cuts at: 1756 1824 1905 Size: 68 81 Fragments arranged by size: 600 400 279 238 149 81 68 61 19 10 BspHI T'CATG_A Cuts at: 0 344 1905 Size: 344 1561 BsrDI GCAATG_nn' Cuts at: 0 1517 1905 Size: 1517 388 BsrFI r'CCGG_y Cuts at: 0 352 617 1279 1905 Size: 352 265 662 626 Fragments arranged by size: 662 626 352 265 BstAPI GCAn_nnn'nTGC Cuts at: 0 1113 1905 Size: 1113 792 BstEII G'GTnAC_C Cuts at: 0 702 1905 Size: 702 1203 BstXI CCAn_nnnn'nTGG Cuts at: 0 960 1029 1905 Size: 960 69 876 Fragments arranged by size: 960 876 69 BstYI r'GATC_y Cuts at: 0 326 548 1331 1905 Size: 326 222 783 574 Fragments arranged by size: 783 574 326 222 Bsu36I CC'TnA_GG Cuts at: 0 908 1741 1905 Size: 908 833 164 Fragments arranged by size: 908 833 164 BtgI C'CryG_G Cuts at: 0 110 206 410 767 1168 1379 1660 1676 Size: 110 96 204 357 401 211 281 16 Cuts at: 1676 1757 1905 Size: 81 148 Fragments arranged by size: 401 357 281 211 204 148 110 96 81 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 51 1143 1905 Size: 51 1092 762 Fragments arranged by size: 1092 762 51 BtsI GCAGTG_nn' Cuts at: 0 788 1905 Size: 788 1117 ClaI AT'CG_AT Cuts at: 0 1370 1905 Size: 1370 535 DrdI GACnn_nn'nnGTC Cuts at: 0 1834 1863 1905 Size: 1834 29 42 Fragments arranged by size: 1834 42 29 EaeI y'GGCC_r Cuts at: 0 92 132 140 516 584 1430 1905 Size: 92 40 8 376 68 846 475 Fragments arranged by size: 846 475 376 92 68 40 8 EagI C'GGCC_G Cuts at: 0 132 1905 Size: 132 1773 EarI CTCTTCn'nnn_ Cuts at: 0 4 1905 Size: 4 1901 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 272 544 1905 Size: 272 272 1361 Fragments arranged by size: 1361 272 272 EcoICRI GAG'CTC Cuts at: 0 1822 1905 Size: 1822 83 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 1905 Size: 694 1211 EcoO109I rG'GnC_Cy Cuts at: 0 57 58 86 87 686 687 902 929 Size: 57 1 28 1 599 1 215 27 Cuts at: 929 965 966 1797 1842 1905 Size: 36 1 831 45 63 Fragments arranged by size: 831 599 215 63 57 45 36 28 27 1 1 1 1 HaeII r_GCGC'y Cuts at: 0 17 231 854 1770 1905 Size: 17 214 623 916 135 Fragments arranged by size: 916 623 214 135 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 604 636 696 728 1411 1443 1905 Size: 604 32 60 32 683 32 462 Fragments arranged by size: 683 604 462 60 32 32 32 KasI G'GCGC_C Cuts at: 0 850 1905 Size: 850 1055 KpnI G_GTAC'C Cuts at: 0 1539 1905 Size: 1539 366 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 279 1905 Size: 279 1626 MscI TGG'CCA Cuts at: 0 518 586 1905 Size: 518 68 1319 Fragments arranged by size: 1319 518 68 MslI CAynn'nnrTG Cuts at: 0 448 1183 1651 1905 Size: 448 735 468 254 Fragments arranged by size: 735 468 448 254 MspA1I CmG'CkG Cuts at: 0 1170 1413 1436 1584 1632 1719 1905 Size: 1170 243 23 148 48 87 186 Fragments arranged by size: 1170 243 186 148 87 48 23 NaeI GCC'GGC Cuts at: 0 354 1905 Size: 354 1551 NarI GG'CG_CC Cuts at: 0 851 1905 Size: 851 1054 NcoI C'CATG_G Cuts at: 0 410 1379 1676 1757 1905 Size: 410 969 297 81 148 Fragments arranged by size: 969 410 297 148 81 NgoMIV G'CCGG_C Cuts at: 0 352 1905 Size: 352 1553 NotI GC'GGCC_GC Cuts at: 0 132 1905 Size: 132 1773 NspI r_CATG'y Cuts at: 0 515 1905 Size: 515 1390 PasI CC'CwG_GG Cuts at: 0 503 978 1905 Size: 503 475 927 Fragments arranged by size: 927 503 475 PciI A'CATG_T Cuts at: 0 511 1905 Size: 511 1394 PflMI CCAn_nnn'nTGG Cuts at: 0 1682 1809 1905 Size: 1682 127 96 Fragments arranged by size: 1682 127 96 PpuMI rG'GwC_Cy Cuts at: 0 902 929 1905 Size: 902 27 976 Fragments arranged by size: 976 902 27 PspOMI G'GGCC_C Cuts at: 0 57 76 86 686 965 1905 Size: 57 19 10 600 279 940 Fragments arranged by size: 940 600 279 57 19 10 PstI C_TGCA'G Cuts at: 0 1104 1905 Size: 1104 801 PvuII CAG'CTG Cuts at: 0 1413 1905 Size: 1413 492 SacI G_AGCT'C Cuts at: 0 1824 1905 Size: 1824 81 SacII CC_GC'GG Cuts at: 0 1171 1905 Size: 1171 734 SanDI GG'GwC_CC Cuts at: 0 902 929 1905 Size: 902 27 976 Fragments arranged by size: 976 902 27 SapI GCTCTTCn'nnn_ Cuts at: 0 4 1905 Size: 4 1901 SexAI A'CCwGG_T Cuts at: 0 473 1905 Size: 473 1432 SfcI C'TryA_G Cuts at: 0 30 1100 1905 Size: 30 1070 805 Fragments arranged by size: 1070 805 30 SfiI GGCCn_nnn'nGGCC Cuts at: 0 113 1905 Size: 113 1792 SfoI GGC'GCC Cuts at: 0 852 1905 Size: 852 1053 SmaI CCC'GGG Cuts at: 0 75 1905 Size: 75 1830 SmlI C'TyrA_G Cuts at: 0 654 877 1905 Size: 654 223 1028 Fragments arranged by size: 1028 654 223 SrfI GCCC'GGGC Cuts at: 0 75 1905 Size: 75 1830 StuI AGG'CCT Cuts at: 0 395 1459 1905 Size: 395 1064 446 Fragments arranged by size: 1064 446 395 StyI C'CwwG_G Cuts at: 0 95 410 1124 1379 1676 1748 1757 1905 Size: 95 315 714 255 297 72 9 148 Fragments arranged by size: 714 315 297 255 148 95 72 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 717 1476 1905 Size: 717 759 429 Fragments arranged by size: 759 717 429 TatI w'GTAC_w Cuts at: 0 782 1619 1905 Size: 782 837 286 Fragments arranged by size: 837 782 286 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 662 694 764 796 1905 Size: 662 32 70 32 1109 Fragments arranged by size: 1109 662 70 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 579 1755 1905 Size: 579 1176 150 Fragments arranged by size: 1176 579 150 XmaI C'CCGG_G Cuts at: 0 73 1905 Size: 73 1832 Enzymes that do cut: AccI Acc65I AclI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PflMI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SfiI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AcuI AflII AgeI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmgBI BmtI BplI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrGI BssHII BssSI BstBI BstZ17I CspCI DraI DraIII EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NheI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI XmnI ZraI