################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BfaI BfrI BglI BlnI BpuAI BsaBI BsaI BseRI BsgI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoRI EcoRV FseI FspI HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluI MunI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 364 GACGTC GACGT^C AflIII 1340 ACGTGT A^C[GA][CT]GT AlwI 996 GGATC GGATC(4/5) AlwNI 177 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 43 GGGCCC GGGCC^C Asp700I 1122 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 139 GAGCAC G[AT]GC[AT]^C AvaI 942 CCCGAG C^[CT]CG[GA]G AvaII 309 GGACC G^G[AT]CC BalI 1208 TGGCCA TGG^CCA BciVI 1359 GTATCC GTATCC(6/5) BclI 884 TGATCA T^GATCA BlpI 72 GCTCAGC GC^T[ACGT]AGC Bpu1102I 72 GCTCAGC GC^T[ACGT]AGC BsaAI 1339 TACGTG [CT]AC^GT[GA] BsiHKAI 139 GAGCAC G[AT]GC[AT]^C BsoBI 942 CCCGAG C^[CT]CG[GA]G Bsp1407I 1343 TGTACA T^GTACA BsrGI 1343 TGTACA T^GTACA BstAPI 1182 GCAATATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1004 TTCGAA TT^CGAA CelII 72 GCTCAGC GC^T[ACGT]AGC EagI 219 CGGCCG C^GGCCG EclXI 219 CGGCCG C^GGCCG Eco52I 219 CGGCCG C^GGCCG EcoT22I 716 ATGCAT ATGCA^T FbaI 884 TGATCA T^GATCA HgaI 160 GACGC GACGC(5/10) MluNI 1208 TGGCCA TGG^CCA MscI 1208 TGGCCA TGG^CCA NdeI 1157 CATATG CA^TATG NsiI 716 ATGCAT ATGCA^T NspI 533 GCATGC [GA]CATG^[CT] NspV 1004 TTCGAA TT^CGAA PstI 556 CTGCAG CTGCA^G SexAI 398 ACCTGGT A^CC[AT]GGT SfuI 1004 TTCGAA TT^CGAA SphI 533 GCATGC GCATG^C SspBI 1343 TGTACA T^GTACA XmnI 1122 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 1257 GGTACC G^GTACC 1540 GGTACC AccI 367 GTCTAC GT^[AC][GT]AC 1336 GTCTAC AccIII 339 TCCGGA T^CCGGA 1019 TCCGGA AcsI 573 GAATTT [GA]^AATT[CT] 659 AAATTC AcyI 160 GACGCC G[GA]^CG[CT]C 364 GACGTC ApoI 573 GAATTT [GA]^AATT[CT] 659 AAATTC Asp718I 1257 GGTACC G^GTACC 1540 GGTACC BanII 43 GGGCCC G[GA]GC[CT]^C 465 GGGCTC BglII 560 AGATCT A^GATCT 620 AGATCT BpmI 289 CTGGAG CTGGAG(16/14) 322 CTGGAG BsaHI 160 GACGCC G[GA]^CG[CT]C 364 GACGTC BsaWI 339 TCCGGA [AT]^CCGG[AT] 1019 TCCGGA BseAI 339 TCCGGA T^CCGGA 1019 TCCGGA BsiEI 219 CGGCCG CG[GA][CT]^CG 1458 CGACCG BsmAI 761 GAGAC GTCTC(1/5) 1428 GTCTC BspEI 339 TCCGGA T^CCGGA 1019 TCCGGA BsrFI 103 ACCGGC [GA]^CCGG[CT] 251 ACCGGC BsrI 416 ACTGG ACTGG(1/-1) 764 ACTGG BstEII 18 GGTGACC G^GT[ACGT]ACC 1026 GGTCACC BstPI 18 GGTGACC G^GT[ACGT]ACC 1026 GGTCACC BstXI 626 CCAACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1058 CCATATCAGTGG Cfr10I 103 ACCGGC [GA]^CCGG[CT] 251 ACCGGC EcoO65I 18 GGTGACC G^GT[ACGT]ACC 1026 GGTCACC Hin1I 160 GACGCC G[GA]^CG[CT]C 364 GACGTC KpnI 1257 GGTACC GGTAC^C 1540 GGTACC MroI 339 TCCGGA T^CCGGA 1019 TCCGGA MseI 1624 TTAA T^TAA 1655 TTAA SfcI 556 CTGCAG C^T[GA][CT]AG 1283 CTACAG SspI 684 AATATT AAT^ATT 1184 AATATT TaqI 568 TCGA T^CGA 1005 TCGA Tru9I 1624 TTAA T^TAA 1655 TTAA Tsp509I 574 AATT ^AATT 660 AATT TthHB8I 568 TCGA T^CGA 1005 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AfaI AluI BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI XhoII