(Circular) (Six-base) MAPSORT of: pFN21AE4521.seq Check: 9115 from: 1 to: 6376 pFN21AE4521 6376 bp With 182 enzymes: SgfI * March 28, 2012 14:08 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2373 4186 278 Size: 53 83 186 936 837 1813 2468 Fragments arranged by size: 2468 1813 936 837 186 83 53 AccI GT'mk_AC Cuts at: 1937 2373 3342 3719 1937 Size: 436 969 377 4594 Fragments arranged by size: 4594 969 436 377 Acc65I G'GTAC_C Cuts at: 3262 3545 3697 3262 Size: 283 152 5941 Fragments arranged by size: 5941 283 152 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2536 4439 5823 6091 1632 Size: 904 1903 1384 268 1917 Fragments arranged by size: 1917 1903 1384 904 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 3345 5275 6252 1203 Size: 2142 1930 977 1327 Fragments arranged by size: 2142 1930 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 2387 2824 3374 1166 Size: 1221 437 550 4168 Fragments arranged by size: 4168 1221 550 437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 2187 4119 5691 2187 Size: 1932 1572 2872 Fragments arranged by size: 2872 1932 1572 ApaI G_GGCC'C Cuts at: 2052 2052 Size: 6376 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 2578 2664 3685 3911 4063 6172 1493 Size: 1085 86 1021 226 152 2109 1697 Fragments arranged by size: 2109 1697 1085 1021 226 152 86 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 2947 3701 1870 Size: 73 1004 754 4545 Fragments arranged by size: 4545 1004 754 73 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2158 2233 3262 3455 3545 Size: 325 217 627 371 75 1029 193 90 Cuts at: 3545 3697 5152 618 Size: 152 1455 1842 Fragments arranged by size: 1842 1455 1029 627 371 325 217 193 152 90 75 BanII G_rGCy'C Cuts at: 1391 2052 2474 3695 1391 Size: 661 422 1221 4072 Fragments arranged by size: 4072 1221 661 422 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3655 3689 4567 4601 1903 Size: 34 50 34 1634 34 878 34 3678 Fragments arranged by size: 3678 1634 878 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3375 4268 5478 3375 Size: 893 1210 4273 Fragments arranged by size: 4273 1210 893 BclI T'GATC_A Cuts at: 1548 2889 1548 Size: 1341 5035 BfrBI ATG'CAT Cuts at: 2723 2723 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2565 2625 6371 2565 Size: 60 3746 2570 Fragments arranged by size: 3746 2570 60 BlpI GC'TnA_GC Cuts at: 2078 3793 2078 Size: 1715 4661 Bme1580I G_kGCm'C Cuts at: 1790 2052 2238 4437 5593 1790 Size: 262 186 2199 1156 2573 Fragments arranged by size: 2573 2199 1156 262 186 BmgBI CAC'GTC Cuts at: 1318 2311 1318 Size: 993 5383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4107 5065 442 Size: 416 53 83 399 24 2690 958 1753 Fragments arranged by size: 2690 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2348 5036 1239 Size: 1076 33 2688 2579 Fragments arranged by size: 2688 2579 1076 33 Bpu10I CC'TnA_GC Cuts at: 2177 2177 Size: 6376 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4501 5366 5664 4501 Size: 865 298 5213 Fragments arranged by size: 5213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3346 4167 493 Size: 711 2142 821 2702 Fragments arranged by size: 2702 2142 821 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 2370 4183 4565 Size: 53 83 186 936 633 204 1813 382 Cuts at: 4565 5200 275 Size: 635 1451 Fragments arranged by size: 1813 1451 936 635 633 382 204 186 83 53 BsaWI w'CCGG_w Cuts at: 2344 3024 4809 5207 5481 5628 6039 2344 Size: 680 1785 398 274 147 411 2681 Fragments arranged by size: 2681 1785 680 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6151 Fragments arranged by size: 6151 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6376 BseYI C'CCAG_C Cuts at: 2352 3157 5579 6075 2352 Size: 805 2422 496 2653 Fragments arranged by size: 2653 2422 805 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5803 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2227 3466 3826 4587 5615 1540 Size: 275 186 226 1239 360 761 1028 2301 Fragments arranged by size: 2301 1239 1028 761 360 275 226 186 BsiHKAI G_wGCw'C Cuts at: 2148 4437 4522 5593 2148 Size: 2289 85 1071 2931 Fragments arranged by size: 2931 2289 1071 85 BsmI GAATG_Cn' Cuts at: 3887 3980 3887 Size: 93 6283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2148 2238 2474 3695 4437 4522 Size: 399 262 96 90 236 1221 742 85 Cuts at: 4522 5593 1391 Size: 1071 2174 Fragments arranged by size: 2174 1221 1071 742 399 262 236 96 90 85 BspEI T'CCGG_A Cuts at: 2344 3024 6039 2344 Size: 680 3015 2681 Fragments arranged by size: 3015 2681 680 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3731 844 Size: 2887 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4871 5045 65 Size: 1183 3623 174 1396 Fragments arranged by size: 3623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2108 2256 5020 5207 1700 Size: 110 298 148 2764 187 2869 Fragments arranged by size: 2869 2764 298 187 148 110 BsrGI T'GTAC_A Cuts at: 96 3348 96 Size: 3252 3124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 3193 794 Size: 2399 3977 BstBI TT'CG_AA Cuts at: 3010 5180 3010 Size: 2170 4206 BstEII G'GTnAC_C Cuts at: 1184 2023 3031 1184 Size: 839 1008 4529 Fragments arranged by size: 4529 1008 839 BstXI CCAn_nnnn'nTGG Cuts at: 2638 3070 2638 Size: 432 5944 BstYI r'GATC_y Cuts at: 1972 2565 2625 3001 3706 4073 4458 4475 5927 Size: 593 60 376 705 367 385 17 1452 Cuts at: 5927 6013 6025 6371 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 705 593 385 376 367 346 86 60 17 12 BtgI C'CryG_G Cuts at: 513 1065 2532 3210 3336 513 Size: 552 1467 678 126 3553 Fragments arranged by size: 3553 1467 678 552 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2254 3158 487 Size: 683 56 1028 904 3705 Fragments arranged by size: 3705 1028 904 683 56 BtsI GCAGTG_nn' Cuts at: 2207 3216 3244 3892 4686 4706 2207 Size: 1009 28 648 794 20 3877 Fragments arranged by size: 3877 1009 794 648 28 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2923 2958 529 Size: 35 2359 35 3947 Fragments arranged by size: 3947 2359 35 35 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2224 2506 3213 Size: 54 1425 50 275 18 394 282 707 Cuts at: 3213 3823 4712 6341 8 Size: 610 889 1629 43 Fragments arranged by size: 1629 1425 889 707 610 394 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2224 3823 1537 Size: 275 412 1599 4090 Fragments arranged by size: 4090 1599 412 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 3230 3254 4306 1353 Size: 1518 359 24 1052 3423 Fragments arranged by size: 3423 1518 1052 359 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2788 4963 5337 5483 387 Size: 1064 138 1199 2175 374 146 1280 Fragments arranged by size: 2175 1280 1199 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2348 2536 4439 5036 5823 6091 Size: 393 683 33 188 1903 597 787 268 Cuts at: 6091 1239 Size: 1524 Fragments arranged by size: 1903 1524 787 683 597 393 268 188 33 EcoO109I rG'GnC_Cy Cuts at: 2048 2049 2428 2048 Size: 1 379 5996 Fragments arranged by size: 5996 379 1 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2062 2085 2187 2288 2792 3205 5523 1678 Size: 384 23 102 101 504 413 2318 2531 Fragments arranged by size: 2531 2318 504 413 384 102 101 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2841 2873 5017 5049 5091 Size: 32 135 32 1269 32 2144 32 42 Cuts at: 5091 5123 1373 Size: 32 2626 Fragments arranged by size: 2626 2144 1269 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3720 3966 669 Size: 988 281 1782 246 3079 Fragments arranged by size: 3079 1782 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3266 3549 3701 3266 Size: 283 152 5941 Fragments arranged by size: 5941 283 152 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 5490 5674 1305 Size: 1350 2835 184 2007 Fragments arranged by size: 2835 2007 1350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3215 10 Size: 54 1425 343 1383 3171 Fragments arranged by size: 3171 1425 1383 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 2537 2824 3038 3068 3122 3374 Size: 648 1221 150 287 214 30 54 252 Cuts at: 3374 3389 3485 3623 4334 4693 4852 518 Size: 15 96 138 711 359 159 2042 Fragments arranged by size: 2042 1221 711 648 359 287 252 214 159 150 138 96 54 30 15 MspA1I CmG'CkG Cuts at: 1606 2151 2244 2326 2491 3259 3792 4469 5617 Size: 545 93 82 165 768 533 677 1148 Cuts at: 5617 5862 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 768 677 545 533 245 165 93 82 NcoI C'CATG_G Cuts at: 513 1065 2532 3210 3336 513 Size: 552 1467 678 126 3553 Fragments arranged by size: 3553 1467 678 552 126 NdeI CA'TA_TG Cuts at: 387 3163 387 Size: 2776 3600 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NsiI A_TGCA'T Cuts at: 2725 2725 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 2542 3734 5279 1132 Size: 97 1313 1192 1545 2229 Fragments arranged by size: 2229 1545 1313 1192 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6376 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspOMI G'GGCC_C Cuts at: 2048 2048 Size: 6376 PstI C_TGCA'G Cuts at: 830 2565 3728 830 Size: 1735 1163 3478 Fragments arranged by size: 3478 1735 1163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2244 2326 2491 3259 2244 Size: 82 165 768 5361 Fragments arranged by size: 5361 768 165 82 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SexAI A'CCwGG_T Cuts at: 2403 2403 Size: 6376 SfcI C'TryA_G Cuts at: 826 1045 2561 3288 3724 4859 5540 5731 826 Size: 219 1516 727 436 1135 681 191 1471 Fragments arranged by size: 1516 1471 1135 727 681 436 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4480 5381 5643 820 Size: 197 926 2537 901 262 1553 Fragments arranged by size: 2537 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 2542 3734 1132 Size: 1410 1192 3774 Fragments arranged by size: 3774 1410 1192 SspI AAT'ATT Cuts at: 5 52 2691 3191 4300 5 Size: 47 2639 500 1109 2081 Fragments arranged by size: 2639 2081 1109 500 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2532 3210 3336 3451 3815 513 Size: 552 1467 678 126 115 364 3074 Fragments arranged by size: 3074 1467 678 552 364 126 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2408 2447 3221 3377 4403 4420 4573 Size: 1255 828 39 774 156 1026 17 153 Cuts at: 4573 4758 5203 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1026 828 774 445 185 156 153 39 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3348 4622 6334 Size: 274 80 33 51 1443 1371 1274 1712 Cuts at: 6334 96 Size: 138 Fragments arranged by size: 1712 1443 1371 1274 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6336 6368 6336 Size: 32 6344 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 3131 4505 3131 Size: 1374 5002 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2371 4184 276 Size: 53 83 186 936 837 1813 2468 Fragments arranged by size: 2468 1813 936 837 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI