Restriction map for
pFN21AB6957
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstF5I BstXI BstYI
BstZ17I CelII ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KasI Ksp632I KspI MaeI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 457 GGTACC G^GTACC
AcyI 543 GACGCC G[GA]^CG[CT]C
AlwNI 240 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 65 AGCGCT AGC^GCT
Asp718I 457 GGTACC G^GTACC
BanI 457 GGTACC G^G[CT][GA]CC
BbsI 612 GAAGAC GAAGAC(2/6)
BpuAI 612 GAAGAC GAAGAC(2/6)
BsaHI 543 GACGCC G[GA]^CG[CT]C
BsmAI 404 GTCTC GTCTC(1/5)
BspHI 407 TCATGA T^CATGA
BspMI 203 GCAGGT ACCTGC(4/8)
Eco47III 65 AGCGCT AGC^GCT
HaeII 65 AGCGCT [GA]GCGC^[CT]
Hin1I 543 GACGCC G[GA]^CG[CT]C
HinfI 428 GAATC G^A[ACGT]TC
KpnI 457 GGTACC GGTAC^C
MboII 612 GAAGA GAAGA(8/7)
MseI 319 TTAA T^TAA
MslI 395 CACTGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 105 CCATGG C^CATGG
NspI 285 GCATGT [GA]CATG^[CT]
PstI 626 CTGCAG CTGCA^G
RcaI 407 TCATGA T^CATGA
SacI 373 GAGCTC GAGCT^C
SfaNI 130 GCATC GCATC(5/9)
SfiI 16 GGCCAGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 251 CCCGGG CCC^GGG
SmlI 28 CTTGAG C^T[CT][GA]AG
TfiI 428 GAATC G^A[AT]TC
Tru9I 319 TTAA T^TAA
XmaCI 251 CCCGGG C^CCGGG
XmaI 251 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 220 CGCG CG^CG
519 CGCG
AfaI 458 GTAC GT^AC
516 GTAC
AocI 57 CCTCAGG CC^T[ACGT]AGG
186 CCTGAGG
ApaI 143 GGGCCC GGGCC^C
150 GGGCCC
AspHI 373 GAGCTC G[AT]GC[AT]^C
600 GAGCAC
AvaI 251 CCCGGG C^[CT]CG[GA]G
547 CCCGAG
BseRI 189 GAGGAG GAGGAG(10/8)
370 GAGGAG
BsiHKAI 373 GAGCTC G[AT]GC[AT]^C
600 GAGCAC
BsoBI 251 CCCGGG C^[CT]CG[GA]G
547 CCCGAG
BsrFI 176 GCCGGT [GA]^CCGG[CT]
194 GCCGGT
BsrI 232 ACTGG ACTGG(1/-1)
396 ACTGG
BstEII 99 GGTAACC G^GT[ACGT]ACC
438 GGTGACC
BstPI 99 GGTAACC G^GT[ACGT]ACC
438 GGTGACC
BstUI 220 CGCG CG^CG
519 CGCG
Bsu36I 57 CCTCAGG CC^T[ACGT]AGG
186 CCTGAGG
Cfr10I 176 GCCGGT [GA]^CCGG[CT]
194 GCCGGT
EaeI 92 TGGCCG [CT]^GGCC[GA]
521 CGGCCA
Eco81I 57 CCTCAGG CC^T[ACGT]AGG
186 CCTGAGG
EcoO65I 99 GGTAACC G^GT[ACGT]ACC
438 GGTGACC
EcoT14I 105 CCATGG C^C[AT][AT]GG
329 CCAAGG
HgaI 282 GACGC GACGC(5/10)
543 GACGC
MvnI 220 CGCG CG^CG
519 CGCG
PflMI 239 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
471 CCAGGGCGTGG
PpuMI 301 GGGACCC [GA]G^G[AT]CC[CT]
559 AGGTCCC
PvuII 125 CAGCTG CAG^CTG
576 CAGCTG
RsaI 458 GTAC GT^AC
516 GTAC
SfcI 573 CTACAG C^T[GA][CT]AG
626 CTGCAG
StyI 105 CCATGG C^C[AT][AT]GG
329 CCAAGG
Van91I 239 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
471 CCAGGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MnlI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV Sau96I ScrFI TseI
Tsp45I TspRI